Dilution
The leukapheresis product must be diluted with CliniMACS
PBS/EDTA Bu
ff
er (supplemented with HSA to a final concentration
of 0.5% (w/v)) before magnetic labeling. Calculate the weight of
bu
ff
er to be added using the following equation and record it.
Weight of buffer
=
Weight of leukapheresis
×
2
to be added (g)
product (g)
1. Take a plasma transfer set and confirm the clamp is in the
closed position. Insert the spike of the plasma transfer set
into a port of the bu
ff
er bag.
2. Using the TSCD, connect the bu
ff
er bag to the Cell
Preparation Bag. Hang the bu
ff
er bag on a bag hanger.
3. Place the Cell Preparation Bag on the balance and tare the
balance.
4. Open the locking forceps next to the Cell Preparation
Bag. Slide the clamp on the plasma transfer set to the
open position. By visually monitoring the scale on the
balance, transfer the calculated weight of bu
ff
er to the Cell
Preparation Bag.
5. When the appropriate weight of bu
ff
er has been
transferred, slide the clamp on the plasma transfer set to
the closed position to stop the liquid flow. Close the locking
forceps next to the Cell Preparation Bag. Record the weight
of bu
ff
er added.
6. Using the heat sealer, seal o
ff
the tubing between
both clamps leaving at least 15 cm of tubing on the Cell
Preparation Bag for further connections. Disconnect the
bu
ff
er bag.
7.
Holding the Cell Preparation Bag with both hands, mix the
contents thoroughly by using a gentle rotating motion.
Avoid intensive mixing of the cells.
8. Tare the balance and weigh the filled Cell Preparation Bag.
Record the weight.
9. Determine the weight of the diluted leukapheresis product
by subtracting the weight of the empty Cell Preparation
Bag from the weight of the filled Cell Preparation Bag.
Record the calculated weight.
Weight of diluted
Weight of filled
Weight of empty
leukapheresis
=
Cell Preparation
–
Cell Preparation
product (g)
Bag (g)
Bag (g)
37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
6 – STEP
1: CliniMACS® CD34 Reagent
