37091/04 – ch20 (Issued: 2020-06)
STEP
1: CliniMACS® CD19 Reagent – 3
STEP
1: CliniMACS® CD19 Reagent
III. Preparative steps
Preparation of the CliniMACS® PBS/EDTA Buffer
Supplement each required liter of CliniMACS® PBS/EDTA Bu
ff
er
with HSA to a final concentration of 0.5 % (w/v), i.e., add 5 g HSA
per liter bu
ff
er.
Labeling and preparation of bags
- Label one 600 mL transfer bag as:
Cell Collection
. (This should
include patient identification, date and time of run, and
operator identification.) Insert a Luer/Spike Interconnector
into the outside port of the Cell Collection Bag. Place a
locking forceps on the tubing close to the bag and close the
locking forceps. Weigh the empty Cell Collection Bag with
locking forceps positioned close to the bag and the tubing
lying on the table next to the balance. Record the weight.
- Label one 600 mL transfer bag as:
Cell Preparation
. (This
should include patient identification, date and time of run,
and operator identification.) Insert a sampling site coupler
into the outside port of the Cell Preparation Bag. Place a
locking forceps on the tubing close to the bag and close the
locking forceps. Weigh the empty Cell Preparation Bag with
locking forceps positioned close to the bag and the tubing
lying on the table next to the balance. Record the weight.
- Label one 600 mL transfer bag as:
Plasma Waste
. (This should
include patient identification, date, time of run, and operator
identification.)
- Label one 600 mL transfer bag as:
Wash Waste
. (This should
include patient identification, date and time of run, and
operator identification.)
Important
■
Note that
HSA is not a component
of the CliniMACS System
. Use only
pharmaceutical grade HSA approved
in your country. Carefully read the
package insert of the HSA used;
in particular the section regarding
hypersensitivity reactions and the
risk of infection that HSA as a blood-
derived product brings to all patients.
All risks arising from these materials
must be evaluated by the user.
■
Store the buffer for cell preparation
at +19 °C to +25 °C (+66 °F to +77 °F).
Lower or higher ambient temperature
will result in less purity and yield of
the target cells.
■
Since the length of the tubing can vary
during the preparation procedure, be
careful when determining the weight
of the Cell Preparation Bag. To acquire
an accurate reading confirm the
locking forceps are always positioned
close to the bag and are lying on the
balance and the rest of the tubing is
lying on the table next to the balance.
