37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
8 – STEP
1: CliniMACS® CD19 Reagent
5. Open the locking forceps next to the Cell Preparation Bag.
Slide the clamp on the plasma transfer set to the open
position. Fill the Cell Preparation Bag with bu
ff
er until the
calculated “Target weight of Cell Preparation Bag filled with
diluted leukapheresis product” is reached. Slide the clamp
on the plasma transfer set to the closed position to stop
the liquid flow. Close the locking forceps next to the Cell
Preparation Bag.
6. Using the heat sealer, seal o
ff
the tubing between both
clamps leaving at least 15 cm of tubing on the Cell
Preparation Bag for further connections. Disconnect the
bu
ff
er bag.
7. Resuspend the cells in the Cell Preparation Bag. Avoid
intensive mixing of the cells. Ensure that all cells are
resuspended.
8. Tare the balance and weigh the filled Cell Preparation Bag.
Record the weight.
9. Determine the weight of the leukapheresis product after
volume adjustment by subtracting the weight of the empty
Cell Preparation Bag from the weight of the filled Cell
Preparation Bag. Record the calculated weight.
Weight of leukaphe-
Weight of
Weight of product
resis after volume
=
filled Cell Pre-
–
empty Cell Pre-
adjustment (g)
paration Bag (g)
paration Bag (g)
10. Keep the Plasma Waste Bag until the final separation and
analysis of all cells have been accomplished.
