37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
10 – STEP
1: CliniMACS® CD19 Reagent
Removal of excess reagent
1. Insert the spike of a plasma transfer set to a port of a bu
ff
er
bag containing at least one liter of bu
ff
er. Confirm the
clamp on the plasma transfer set is in the closed position.
2. Using the TSCD, connect the bu
ff
er bag to the Cell
Preparation Bag. Hang the bu
ff
er bag on a bag hanger.
3. Tare the balance. Place the Cell Preparation Bag on the
balance.
4. Open the locking forceps next to the Cell Preparation Bag.
Slide the clamp on the plasma transfer set to the open
position. Completely fill the Cell Preparation Bag with
bu
ff
er. Slide the clamp on the plasma transfer set to the
closed position to stop the liquid flow. Close the locking
forceps next to the Cell Preparation Bag. Record the weight
of the filled Cell Preparation Bag.
5. Using the heal sealer, seal o
ff
the tubing between both
clamps leaving at least 15 cm of tubing on the Cell
Preparation Bag for further connections. Disconnect the
bu
ff
er bag.
6. Holding the Cell Preparation Bag with both hands, mix the
contents (leukapheresis product and bu
ff
er) thoroughly by
using a gentle rotating motion.
7. Using the TSCD, connect the empty Wash Waste Bag to the
Cell Preparation Bag.
8. Fold any loose parts of the bags or tubing downwards.
Transfer the Cell Preparation Bag and Wash Waste Bag
securely to the centrifuge bucket.
9. Balance the loaded bucket with a suitable weighted bucket.
It is essential that the centrifuge is balanced accurately.
10. Centrifuge at 300
×
g (without brake) for 15 minutes at room
temperature (+19 °C to +25 °C [+66 °F to +77 °F]).
11. Taking care not to disturb the cell pellet, remove the bags
from the centrifuge.
12. Carefully hang the Cell Preparation Bag on the plasma
extractor.
