37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
10 – STEP 1: CliniMACS® TCR
α
/
β
-Biotin
4. Open the locking forceps next to the Cell Preparation Bag.
Slide the clamp on the plasma transfer set to the open
position. Completely fill the Cell Preparation Bag with
bu
ff
er. Slide the clamp on the plasma transfer set to the
closed position to stop the liquid flow. Close the locking
forceps next to the Cell Preparation Bag. Record the weight
of the filled Cell Preparation Bag.
5. Using the heat sealer, seal o
ff
the tubing between
both clamps leaving at least 15 cm of tubing on the Cell
Preparation Bag for further connections. Disconnect the
bu
ff
er bag.
6. Holding the Cell Preparation Bag with both hands, mix the
contents (leukapheresis product and bu
ff
er) thoroughly by
using a gentle rotating motion.
7.
Using the TSCD, connect the empty Wash Waste Bag No. 1
to the Cell Preparation Bag.
8. Fold any loose parts of the bags or tubing downwards.
Transfer the Cell Preparation Bag and Wash Waste Bag No. 1
securely to the centrifuge bucket.
9. Balance the loaded bucket with a suitable weighted bucket.
It is essential that the centrifuge is balanced accurately.
10. Centrifuge at 300
×
g (without brake) for 15 minutes at room
temperature (+19 °C to +25 °C [+66 °F to +77 °F]).
11. Taking care not to disturb the cell pellet, remove the bags
from the centrifuge.
12. Carefully hang the Cell Preparation Bag on the plasma
extractor.
13. Place the Wash Waste Bag No. 1 on the balance and tare the
balance.
14. Open the locking forceps next to the Cell Preparation
Bag. Using the plasma extractor, remove as much excess
supernatant as possible from the Cell Preparation Bag.
Close the locking forceps next to the Cell Preparation Bag
to stop the liquid flow.
15. Using the heat sealer, seal o
ff
the tubing leaving at least
15 cm of tubing on the Cell Preparation Bag for further
connections. Disconnect the Wash Waste Bag No. 1.
16. Keep the Wash Waste Bag No. 1 until the separation and
final analysis of cells have been accomplished.
