37091/04 – ch20 (Issued: 2020-06)
CliniMACS® Plus System User Manual (Canadian edition)
14 – STEP 1: CliniMACS® TCR
α
/
β
-Biotin
14. Open the locking forceps next to the Cell Preparation Bag.
Using the plasma extractor, remove as much supernatant
as possible from the Cell Preparation Bag. Close the locking
forceps next to the Cell Preparation Bag to stop the liquid
flow.
15. Using the heat sealer, seal o
ff
the tubing leaving at least
15 cm of tubing on the Cell Preparation Bag for further
connections. Dis con nect the Wash Waste Bag No. 3.
16. Keep the Wash Waste Bag No. 3 until the separation and
final analysis of all cells has been completed.
17. Resuspend the cell pellet in the Cell Preparation Bag.
Avoid intensive mixing of the cells. Ensure that all cells are
resuspended.
18. Tare the balance and weigh the filled Cell Preparation Bag.
Record the weight.
19. Determine the weight of the leukapheresis product
after the wash by subtracting the weight of the empty
Cell Preparation Bag from the weight of the filled Cell
Preparation Bag. Record the calculated weight.
Weight of leuka-
Weight of filled
Weight of empty
pheresis product
=
Cell Preparation
–
Cell Preparation
after the wash (g)
Bag (g)
Bag (g)
20. Adjust sample loading volume: Calculate the weight of
bu
ff
er necessary to adjust the weight of the cell suspension
to approximately 150 g. For loading the labeled and washed
cells on the tubing set, a maximum cell concentration of
0.4
×
10
9
cells per mL is recommended.
Weight of
Weight of leuka-
buffer to be
=
150 g
–
pheresis product
to be added (g)
after the wash (g)
21. Using the TSCD, connect the bu
ff
er bag to the Cell Pre-
paration Bag. Hang the bu
ff
er bag on a bag hanger.
22. Place the Cell Preparation Bag on the balance and tare the
balance.
23. Open the locking forceps next to the Cell Preparation
Bag. Slide the clamp on the plasma transfer set to the
open position. By visually monitoring the scale on the
balance, transfer the calculated weight of bu
ff
er to the Cell
Preparation Bag.
