Miltenyi Biotec MultiMACS Cell24 Separator Plus, User Manual

Page 1: ...MultiMACS Cell24 Separator Plus User manual...

Page 2: ...ion of this user manual However Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions contained herein or for incidental or consequential damages in connection with th...

Page 3: ...Separator Plus User manual Original instructions Miltenyi Biotec B V Co KG Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 0 Fax 49 2204 85197 macsde miltenyi com www mil...

Page 4: ...could lead to improper or incorrect usage and result in damage to the instrument Improper usage could also cause severe personal injury death unpredictable results instrument malfunction and premature...

Page 5: ...ly for benchtop use 44 3 Operating the instrument 47 3 1 Switching on and off 47 3 2 Stopping a moving magnet 47 3 3 Menu 48 3 3 1 Start menu 48 3 3 2 Process menu 49 3 3 3 Set up 49 3 3 4 New process...

Page 6: ...ocesses 65 6 1 Creating a process 65 6 2 Replacing a process 67 6 3 Editing a process 67 6 3 1 Saving as new process 67 6 3 2 Changing parameters 68 6 4 Deleting a process 68 6 5 Testing plate height...

Page 7: ...led cells 78 8 5 Poor elution recovery 78 8 6 Error codes 78 9 Technical data and specifications 79 9 1 Technical data 80 9 2 EC EU Declaration of Conformity 82 9 2 1 MultiMACS Cell24 Separator 82 9 2...

Page 8: ...6...

Page 9: ...ct the local authorities governing electrical power supply building constructions maintenance or safety for more information about the safe installation and operation of the instrument Hazard levels S...

Page 10: ...EQUIPMENT READ THE USER MANUAL BEFORE USING THE INSTRUMENT MANUFACTURER ORDER NUMBER TYPE NUMBER SERIAL NUMBER Safety labels Notice the hazard points and safety symbols of the instrument Keep safety...

Page 11: ...sons carrying pacemakers or electronic medical implants stay clear Keep all magnetically sensitive objects away from device Safety label 2 and 4 CAUTION Tray in motion during operation Hazard of crush...

Page 12: ...wer cable l an object has been entered through the ventilation slots l an object has been dropped into the instrument If flames or smoke appear immediately switch off the power supply unplug the instr...

Page 13: ...iMACS Cell24 Magnet Moreover the MultiMACS Display also contains powerful magnets There is a risk of severe personal injury for persons carrying pacemakers brain shunts or electronic medical implants...

Page 14: ...otherwise specifically noted in this user manual or other Miltenyi Biotec documentation do not service the instrument yourself Servicing and repair must be performed by qualified service personnel Im...

Page 15: ...WEEE and hazardous waste disposal legislation you are helping to preserve our natural resources and protect human health Miltenyi Biotec is committed to protecting the environment Miltenyi Biotec offe...

Page 16: ...IMPORTANT SAFETY INFORMATION EN DE ES FR 14...

Page 17: ...n werden Wenden Sie sich f r weitere Informationen zur Stromversorgung Geb udeinstallationen Wartung und Sicherheit f r die Installation dieses Ger tes an die rtlichen Beh rden und Ihren Stromversorge...

Page 18: ...LESEN SIE DAS BENUTZERHANDBUCH VOR VERWENDUNG DES GER TES HERSTELLER BESTELLNUMMER MODELLNUMMER SERIENNUMMER Sicherheitskennzeichnungen Beachten Sie die Gefahrenhinweise und Sicherheitssymbole des Ge...

Page 19: ...der elektronischen medizinischen Implantaten Abstand halten Alle magnetisch empfindlichen Gegenst nde vom Ger t fernhalten Sicherheitsaufkleber 2 und 4 Vorsicht Halter bewegt sich w hrend des Betriebs...

Page 20: ...em Personal entfernt werden F hren Sie nie einen Fremdk rper durch eine ffnung in das Ger t ein Benutzen Sie das Ger t nicht wenn l es offen oder zerlegt ist l es fallengelassen wurde oder besch digt...

Page 21: ...auf dem Typschild entspricht Sollten Sie Fragen zur Art der Stromversorgung haben wenden Sie sich an einen autorisierten Miltenyi Biotec Servicedienstleister oder Ihren lokalen Stromversorger Benutzen...

Page 22: ...ichende Schutzausr stung ist gef hrlich Werden Gefahrstoffe oder unbekannte Substanzen eingesetzt muss das Ger t unter einem Abzug oder einer Sicherheitswerkbank benutzt werden Falls Gefahrstoffe verw...

Page 23: ...ectrical and Electronic Equipment WEEE Bitte entsorgen Sie Ihre Altger te von Miltenyi Biotec unter Einhaltung der jeweils geltenden Vorschriften f r die Erfassung und Behandlung von Elektro und Elekt...

Page 24: ...WICHTIGE SICHERHEITSINFORMATIONEN EN DE ES FR 22...

Page 25: ...s P ngase en contacto con la autoridad local a cargo de la fuente de energ a el ctrica construcciones mantenimiento o seguridad para m s informaci n sobre la instalaci n segura y manejo del instrument...

Page 26: ...CTRICOS Y ELECTR NICOS LEA EL MANUAL DE USUARIO ANTES DE USAR EL INSTRUMENTO FABRICANTE N MERO DE PEDIDO N MERO DEL MODELO N MERO DE SERIE Etiqueta de seguridad Observe los puntos de peligro y los s...

Page 27: ...s o implantes m dicos electr nicos mant ngase alejado Mantenga lejos del dispositivo todos los objetos magn ticamente sensibles Etiqueta de seguridad 2 y 4 ATENCI N Soporte en movimiento durante el fu...

Page 28: ...quite o penetre ninguna tapa o cubierta Solo el personal autorizado debe quitar las otras tapas del instrumento Nunca introduzca un objeto extra o a trav s de una apertura en el instrumento No use el...

Page 29: ...que cumple las especificaciones mencionadas en la etiqueta de potencia el ctrica del producto Si tiene preguntas sobre el tipo de fuente de energ a que usar p ngase en contacto con su proveedor de se...

Page 30: ...iz pues los aerosoles podr an salir del sistema Un equipamiento de seguridad defectuoso o inadecuado es peligroso El instrumento debe ser utilizado con una cubierta de seguridad si est n siendo proces...

Page 31: ...Los equipos el ctricos y electr nicos pueden contener sustancias peligrosas que pueden tener graves efectos perjudiciales sobre el medio ambiente y o la salud humana Por este motivo todos los equipos...

Page 32: ...INFORMACI N IMPORTANTE DE SEGURIDAD EN DE ES FR 30...

Page 33: ...ion d accidents Contactez les autorit s locales g rant l alimentation en courant lectrique la construction de b timents la maintenance ou la s curit pour plus d informations sur l installation et l ut...

Page 34: ...D QUIPEMENTS LECTRIQUES ET LECTRONIQUES LISEZ LE GUIDE DE L UTILISATEUR AVANT D UTILISER L INSTRUMENT FABRICANT NUM RO DE COMMANDE NUM RO DE MOD LE NUM RO DE S RIE tiquette de s curit Rep rez les poin...

Page 35: ...sque de blessure grave Elles doivent rester distance Maintenir tous les objets sensibles aux champs magn tiques distance du dispositif tiquette de s curit 2 et 4 ATTENTION Plateau en mouvement pendant...

Page 36: ...oit d enlever les autres couvercles N introduisez jamais un corps trangers travers d une fente l int rieure de l appareil N utilisez jamais l appareil lorsqu l il est ouvert ou d mont l il est tomb ou...

Page 37: ...questions sur le type de source de courant utiliser contactez votre prestataire de services Miltenyi Biotec agr ou votre entreprise locale de production et de distribution d nergie N utilisez aucune...

Page 38: ...it tre utilis dans une hotte de s curit si des mati res dangereuses ou inconnues sont trait es Si des mati res dangereuses ont t utilis es ou renvers es l appareil doit soigneusement et compl tement t...

Page 39: ...iotec en fin de vie en conformit avec la directive DEEE en vigueur dans votre pays Les quipements lectriques et lectroniques peuvent contenir des substances dangereuses qui peuvent avoir un effet n fa...

Page 40: ...INFORMATIONS DE S CURIT IMPORTANTES EN DE ES FR 38...

Page 41: ...giving reliable and standardized results It is compatible with many downstream applications such as molecular and functional assays chimerism analysis and flow cytometry Positive or untouched isolati...

Page 42: ...of the MultiMACS Cell24SeparatorPlus Figure 1 3 Connectors on the rearside of the MultiMACS Cell24Separator Figure 1 4 Rearside of the MACS Elution Station with connectors left and vacuum chamber rig...

Page 43: ...1 INTRODUCTION 41 Figure 1 5 MACS Elution Chamber including a 24 well Deep Well Plate and lid I Figure 1 6 MACS Elution Chamberwith lid II...

Page 44: ...1 INTRODUCTION 42...

Page 45: ...parator Plus 130 098 637 Component MultiMACS Cell24 Separator MACS Elution Station MACS Elution Chamber including lid I for elution into standard deep well plates and lid II for elution into 5 mL tube...

Page 46: ...ultiMACS Cell24SeparatorPlus consumables Use deep well plates that comply to ANSI SBS standards length 127 76 mm 0 5 mm width 85 48 mm 0 5 mm As height of the deep well plates a value of 44 0 mm is se...

Page 47: ...the MultiMACS Cell24 Separator Fasten the plugs with the screws 7 Connect the MACS Elution Chamber and the MACS Elution Station with the delivered tubing The socket on the rear panel of the MACS Elut...

Page 48: ...rator Plus can be integrated into liquid handling systems for automated use For further information refer to the Miltenyi Biotec Technical Support or visit www miltenyibiotec com for our instrument po...

Page 49: ...ctric sensor detects the tilted position and the movement is stopped An error message will appear on the screen Before starting automated processes with the MultiMACS Cell24 Separator Plus which has b...

Page 50: ...cess 3 3 Menu The following chart shows the software menu structure of the MultiMACS Cell24 Separator Plus Figure 3 1 The software menu structure 3 3 1 Start menu In the Start menu the following three...

Page 51: ...e following set up options are available l Exchange the MultiMACS Cell24 Magnet l Lock the MultiMACS Cell24 Magnet for transport l Show the firmware version l Turn on or off the sound Figure 3 4 Set u...

Page 52: ...ment of the magnet has been stopped due to a blockage Tap this button after the blocking object has been removed TEST Test the plate height SKIP Skip the vacuum elution step 3 5 Instructions for use T...

Page 53: ...ACS Single Column Adpater can be only inserted in one direction 3 5 2 Placing plates 1 The MultiMACS Cell24 Magnet and the MultiMACS Column Holder move up 2 The instrument prompts you to insert change...

Page 54: ...from the column tips The plate can be moved back and forth to deposit the last drop into the wells This ensures reproducible recoveries and avoids cross contamination The column tips must protrude 1...

Page 55: ...Place the lid on top of the MACS Elution Chamber Use lid I for elution into 24 well deep well plates and lid II for the elution into 5 mL tubes The lid of the MACS Elution Chamber can be only inserted...

Page 56: ...to the Process menu of the process you want to run 2 Tap the Run button to start the process The MultiMACS Cell24 Magnet will move to the start position 3 Follow the instructions on the screen 3 7 Vie...

Page 57: ...tive fraction l POSSEL2 and POSSEL2_SCA for the collection of labeled and unlabeled cells positive and negative fraction Use POSSEL and POSSEL_2 for separations with the Multi 24 Column Block and POSS...

Page 58: ...or more than 12 samples use the Multi 24 Column Block refer to Figure 4 1 The capacity of MACS Columns including LD LS and Whole Blood Columns differs from the capacity of manual use refer to Table 4...

Page 59: ...n POSSEL2 POSSEL2_SCA 3 Equilibration buffer Unlabeled fraction Labeled fraction Table 4 2 Numberof deep well plates required forpre defined processes For waste collection use deep well plates The def...

Page 60: ...in two steps onto the column Two 44 0 mm deep well plates are required for the collection of equilibration buffer flow through and wash Alternatively use a 88 0 mm deep well plate 1 Apply the first 5...

Page 61: ...Adapter onto the MultiMACS Column Holder and insert the columns Tap OK Ensure that all columns are well fitted into the MultiMACS Single Column Adapter after movement of the MultiMACS Cell24 Magnet 3...

Page 62: ...columns The deep well plate can be inserted in two directions To ensure the correct assignment of the samples note the orientation of the deep well plate to the columns 9 Place lid I on the base of t...

Page 63: ...on lid I 13 Tap OK to apply the elution impulse 14 APPLY ELUTION BUFFER Apply elution buffer onto each column to elute the labeled cells 15 Let the elution buffer run through completely Tap OK 16 Tap...

Page 64: ...2 Tap OK to apply the elution impulse 13 Tap the MultiMACS Single Column Adapter with the MACS Columns to the MACS Elution Chamber to collect drops from the column tips and ensure complete recovery of...

Page 65: ...well plate into the base of the MACS Elution Chamber to collect the positive fraction from the columns The deep well plate can be inserted in two directions To ensure the correct assignment of the sa...

Page 66: ...5 PRE DEFINED PROCESSES 64...

Page 67: ...K 3 Enter a name for the new process Up to 13 characters can be used The letters are arranged in triplets Tap one two or three times to select a letter Tap for further letters symbols and numerals Tap...

Page 68: ...s refer to chapter Testing plate height and distance for plate removal on page 68 11 Select a container for the unlabeled fraction This parameter is only available when the target fraction is the nega...

Page 69: ...turn to the Start menu 6 3 Editing a process The pre defined processes DEPLETE POSSEL POSSEL2 POSSEL_SCA and POSSEL2_SCA must be saved as a new process after changing the process parameters Edited par...

Page 70: ...p SAVE PROCESS TO LIST or MORE CHANGES to return to the edit function 4 After the process has been saved tap YES to start the process or tap NO or ESC to return to the Start menu 6 4 Deleting a proces...

Page 71: ...net will move down to the next position 4 Check whether the height is acceptable by moving the Tip Touch Plate back and forth The column tips must be inserted slightly in the well 1 mm Tap OK and the...

Page 72: ...6 CUSTOM PROCESSES 70...

Page 73: ...each for plates columns or any item between the Tip Touch Plate and the magnet Do not place any bodily parts between the magnet and the MultiMACS Column Holder while the magnet is moving 7 1 Cleaning...

Page 74: ...ther MultiMACS Cell24 Magnet and Display at a safe distance Keep away from all moving parts The MultiMACS Cell24 Magnet and MultiMACS Column Holder are mounted in landscape orientation by default If n...

Page 75: ...are always visible 9 Rotate the MultiMACS Column Holder the same way as the MultiMACS Cell24 Magnet Note the orientation of the holding areas for the Multi 24 Column Block or MultiMACS Single Column A...

Page 76: ...g 5 20 mm T3 15AH250V 4 Reinsert the fuse holder 7 4 Firmware version Before contacting Miltenyi Biotec Technical Support determine the version of the firmware on the MultiMACS Cell24 Separator Plus 1...

Page 77: ...the lock position 5 When MULTIMACS SEP IS READY FOR TRANSPORT SWITCH DEVICE OFF is shown switch off the instrument 6 Detach the Tip Touch Plate 7 Disconnect the MACS Elution Chamber Push the tube con...

Page 78: ...7 MAINTENANCE 76...

Page 79: ...ll24 Separator Plus is registered while the MultiMACS Cell24 Magnet is moving the movement stops and the following screen is shown 1 If no blocking objects are visible tap RETRY 2 If a blocking object...

Page 80: ...ONG MAG POS ERROR 102 INITIALIZING SYSTEM Incorrect positioning of the MultiMACS Cell24 Magnet Tap OK for intermediate reinitialization of the system WRONG COL POS ERROR 106 INITIALIZING SYSTEM The Mu...

Page 81: ...diate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference wi...

Page 82: ...in 8 RTS Pin 9 5 V CAN with high power contacts labeled module 1 Pin 3 4 5 NC Pin A1 24 V Pin A2 GND Pin 1 CAN_L Pin 2 CAN_H Standard CAN labeled module 2 Pin 1 4 5 8 NC Pin 2 CAN_L Pin 3 6 GND Pin 7...

Page 83: ...A1 24 V Pin A2 GND Pin 1 CAN_L Pin 2 CAN_H Pin 3 GND Display Interface labeled RS232 Display Pin 1 4 6 7 8 NC Pin 2 RXD Pin 3 TXD Pin 5 GND Pin 9 5 V USB Interface labeled USB Pin 1 5 V Pin 2 D Pin 3...

Page 84: ...formity refers to the product identified as follows Description Laboratory equipment Model MultiMACS Cell24 Separator The machinery complies with all essential requirements of the following directives...

Page 85: ...he declaration of conformity refers to the product identified as follows Description Laboratory equipment Model MACS Elution Station The machinery complies with all essential requirements of the follo...

Page 86: ...9 TECHNICAL DATA AND SPECIFICATIONS 84...

Page 87: ...sentative or Miltenyi Biotec Technical Support at Miltenyi Biotec headquarters Miltenyi Biotec B V Co KG Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 830 Fax 49 2204 83...

Page 88: ...10 TECHNICAL SUPPORT 86...

Page 89: ...eturn the nonconforming or defective product to Miltenyi Biotec freight prepaid only after receipt of a Return Material Authorization RMA from Miltenyi Biotec which may include biohazard decontaminati...

Page 90: ...VIDED IN THIS WARRANTY STATEMENT MILTENYI BIOTEC HEREBY DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESS OR IMPLIED ORAL OR WRITTEN WITH RESPECT TO THE PRODUCT INCLUDING WITHOUT LIMITATION ALL IMPLIED W...

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Page 92: ...i Co Ltd Room 401 No 1077 Zhangheng Road Pudong New Area 201203 Shanghai P R China Phone 86 21 62351005 Fax 86 21 62350953 macscn miltenyi com France Miltenyi Biotec SAS 10 rue Mercoeur 75011 Paris Fr...