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5
Pre-defined processes
Do not place columns into the MultiMACS Cell24 Separator Plus until the prompt screen
INSERT COLUMNS
appears to ensure that there are no moving parts when placing the columns. This will prevent injuries to
fingers that can otherwise become trapped between the MultiMACS Cell24 Magnet and the MultiMACS
Column Holder.
The following five processes are pre-defined on the MultiMACS Cell24 Separator Plus. The programs are suitable
for collection of the unlabeled fraction in 24-well deep well plates. For the collection in 5 mL tubes create a
customized process (refer to
).
For equilibration, wash, and elution use the buffer volumes as indicated in
5.1
DEPLETE
1
Select
DEPLETE
in the
Start
menu. The
Process
menu opens. Tap the
Run
button.
2 INSERT COLUMNS
: Insert the Multi-24 Column Block into the MultiMACS Column Holder. If you use single
columns, place the MultiMACS Single-Column Adapter onto the MultiMACS Column Holder and insert the
columns. Tap
OK
.
Ensure that all columns are well fitted into the MultiMACS Single-Column Adapter after movement of the
MultiMACS Cell24 Magnet.
3 INSERT WASTE PLATE
: Insert a deep well plate onto the Tip-Touch Plate. Tap
OK
.
4 APPLY EQUILIBRATION BUFFER
: Apply degassed equilibration buffer onto each column and let the
buffer run through. Tap
OK
.
5 TIP-TOUCH COLUMNS IN PLATE
: Move the Tip-Touch Plate firmly back and forth once. Tap
OK
.
6 INSERT PLATE TO COLLECT UNLABELED FRACTION
: Remove the waste plate and insert a new deep
well plate onto the Tip-Touch Plate. Tap
OK
.
7 APPLY SAMPLE AND WASH
: Apply the suspension of magnetically labeled cells onto each column and
let it run through.
8
Apply degassed wash buffer onto each column and let it run through. Ensure that the column reservoir is
empty before applying new wash buffer. Tap
OK
.
9 TIP-TOUCH COLUMNS IN PLATE
: Move the Tip-Touch Plate firmly back and forth once. Tap
OK
. The
combined flow-through from the cell suspension and the washes represent the unlabeled target cell
fraction.
10 REMOVE PLATE WITH UNLABELED FRACTION
: Remove the deep well plate and tap
OK
.
Summary of Contents for MultiMACS Cell24 Separator Plus
Page 1: ...MultiMACS Cell24 Separator Plus User manual...
Page 8: ...6...
Page 16: ...IMPORTANT SAFETY INFORMATION EN DE ES FR 14...
Page 24: ...WICHTIGE SICHERHEITSINFORMATIONEN EN DE ES FR 22...
Page 32: ...INFORMACI N IMPORTANTE DE SEGURIDAD EN DE ES FR 30...
Page 40: ...INFORMATIONS DE S CURIT IMPORTANTES EN DE ES FR 38...
Page 44: ...1 INTRODUCTION 42...
Page 66: ...5 PRE DEFINED PROCESSES 64...
Page 72: ...6 CUSTOM PROCESSES 70...
Page 78: ...7 MAINTENANCE 76...
Page 86: ...9 TECHNICAL DATA AND SPECIFICATIONS 84...
Page 88: ...10 TECHNICAL SUPPORT 86...
Page 91: ......
