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2 Introduction
Introduction
MultiMACS M96 Separator
The MultiMACS M96 Separator is a magnetic separator specifically
developed for molecular biology applications, like nucleic acid
purification or protein isolation, in microtiter plate format. Thus,
Miltenyi Biotec’s magnetic bead technology is easily applicable for
automated high-throughput procedures.
The MultiMACS M96 Separator can be employed as a semi-automated
bench-top instrument or integrated into fully automated
liquid-handling platforms. A strong 96-well magnet is used to bind
and purify magnetically labeled biomolecules with Multi-96 Columns
or Multi-8 Columns. Then, target molecules are eluted.
The key components of the MultiMACS M96 Separator are shown in
figure 1.
The MultiMACS 96 Magnet generates a high-gradient magnetic field
to bind magnetically labeled molecules in the columns. The
MultiMACS Column Holder does hold a MultiColumn frame with
up to twelve Multi-8 Columns (up to 96 columns).
The MultiMACS 96 Magnet can be moved vertically (up and down)
together with or independent from the MultiMACS Column Holder.
Thereby, either lowering of the columns into the wells of the magnet
or the lifting of the columns out of the magnet is performed. The
vertical movements of the Magnet and the Column Holder are driven
by a motor, which is placed inside of the MultiMACS M96 Separator.
The motor is controlled either manually by utilizing the touchscreen
or fully automated using the RS232-Interface. Note that the
orientation of the MultiMACS 96 Magnet and the MultiMACS Column
Holder can be changed from landscape to portrait and vice versa.
During a separation or purification process with the MultiMACS M96
Separator, liquids such as samples, wash and elution buffers are
dispensed onto the columns either manually, e.g., by an 8-channel
pipette or automated by the liquid-dispensing device of a robot.
For manual processes, the plates are placed onto the Tip-Touch Plate,
which can be moved horizontally back and forth to effectively
deposit the last drop into the wells (tip-touch). In automated
processes, the plates are moved by a gripper to accomplish this.
The Protective Screen is used to prevent injuries to fingers that can
Summary of Contents for MultiMACS M96
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