6
LA
SE
R
A
LI
G
N
M
EN
T
31
4
Check by eye that the alignment tool is in a centered position, and move it into the light sheets by using
the jog wheel. The light sheets from the right-hand side should pass the pinhole and form thin lines on the
other side. The sharper the lines, the better is the z-position.
5
Decrease the sheet width to the standard, refer to
Setting the parameters for the alignment on
.
6
Lower the objective lens, and focus on the light sheets.
7
Click the
Show crosshair
button on the right side of the live window to activate the crosshair.
8
Place the Rayleigh length in the center of the field of view by clicking the
Rayleigh ROI
button under the
Measurement
tab in the
Settings 1
window.
Summary of Contents for UltraMicroscope II
Page 1: ...UltraMicroscope II Imaging System User manual...
Page 14: ...1 IMPORTANT SAFETY INFORMATION 12...
Page 20: ...2 INTRODUCTION 18...
Page 30: ...5 PREPARING THE INSTRUMENT 28...
Page 38: ...6 LASER ALIGNMENT 36...
Page 46: ...7 SETTING UP AN EXPERIMENT 44...
Page 48: ...8 MAINTENANCE 46...
Page 54: ...10 TECHNICAL SUPPORT 52...
Page 57: ......
