Miltenyi Biotec UltraMicroscope II, User Manual

Page 1: ...UltraMicroscope II Imaging System User manual...

Page 2: ...ess or organization Maximal care has been taken by Miltenyi Biotec in the preparation of this user manual However Miltenyi Biotec shall not be liable for any technical or editorial errors or omissions...

Page 3: ...by Miltenyi Biotec B V Co KG Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 0 Fax 49 2204 85197 macsde miltenyi com www miltenyibiotec com LaVision BioTec GmbH a Miltenyi...

Page 4: ...could lead to improper or incorrect usage and result in damage to the instrument Improper usage could also cause severe personal injury death unpredictable results instrument malfunction and prematur...

Page 5: ...rface 17 3 Installation 19 3 1 Components Zoom Body configuration 19 3 2 Accessories Zoom Body configuration 20 3 3 Components Super Plan configuration 20 3 4 Accessories Super Plan configuration 21 3...

Page 6: ...e color z stack 37 7 2 1 Aligning the sample 37 7 2 2 Optimizing the measurement settings 39 7 2 3 Capturing an image 40 7 3 Capturing a multi color z stack 41 7 4 Capturing a mosaic image 42 8 Mainte...

Page 7: ...ety and prevention of accidents Ensure safe working conditions and provide for sufficient light Safe working is only possible if solely trained personnel uses the instrument The training is a permanen...

Page 8: ...fe practices or NOTICE indicates information considered important but not hazard related e g messages relating to property damage Symbols SAFETY WARNING THE DOCUMENTATION MUST BE CONSULTED IN ALL CASE...

Page 9: ...e not legible or identifiable from a safe viewing distance Contact Miltenyi Biotec for replacement labels Figure 1 1 Hazard areas and safety symbols on the top side of the UltraMicroscope II Imaging S...

Page 10: ...se the instrument if liquids have entered the inside of the instrument Protect the instrument against spilled liquids Do not allow liquids to enter the inside of the instrument Clean up spilled liquid...

Page 11: ...azardous Follow local regulations regarding waste disposal Equipment damage Defective or inadequate equipment can cause a biological hazard Some decontamination methods may damage the instrument If pa...

Page 12: ...at support a weight of 250 kg Do not install the instrument yourself Only trained Miltenyi Biotec personnel or personnel authorized by Miltenyi Biotec is allowed to install the instrument ERGONOMIC HA...

Page 13: ...ltenyi Biotec products in accordance with the applicable WEEE and hazardous waste disposal legislation which may differ by country or region Electrical equipment may contain hazardous substances that...

Page 14: ...1 IMPORTANT SAFETY INFORMATION 12...

Page 15: ...with one of the following modules l the Zoom Body Module for easy handling in a multi user environment l the Super Plan Module that can deliver unprecedented image resolution and image quality and an...

Page 16: ...ap available For the Super Plan configuration there are two laser configurations and two camera configurations available There are three different objectives and four dipping caps available This guara...

Page 17: ...y configuration 1 Cable duct 3 Type label 2 Cable duct 4 Electronic interface 2 3 3 Front view Super Plan configuration Figure 2 3 Front view of the UltraMicroscope II Imaging System Super Plan config...

Page 18: ...view Super Plan configuration Figure 2 4 Rear view of the UltraMicroscope II Imaging System Super Plan configuration 1 Cable duct 4 Type label 2 Controller connection 5 I O ports 3 Cable duct 2 INTRO...

Page 19: ...nic interface Figure 2 5 Detail view of the I O ports and power switch 1 Power supply 4 Camera trigger 2 Jog wheel 5 PC connection 3 Detection unit 6 Power switch Figure 2 6 Detail view of the couplin...

Page 20: ...2 INTRODUCTION 18...

Page 21: ...sales representative Components included in the delivery Beam Combiner 150 000 484 White Light Laser 150 001 844 Light source up to 5 lasers of choice white light laser Objective lens 2x 2x Emission...

Page 22: ...ument Additional accessories and consumables developed specifically for the system are available separately Refer to Table 3 4 and Table 3 5 For details visit www miltenyibiotec com or contact your lo...

Page 23: ...g cap water for objective lens 4 MI PLAN 150 001 660 Dipping cap aqueous buffers for objective lens 4 MI PLAN 150 001 658 Dipping cap organic solvents for objective lens 4x MI PLAN 150 001 657 Dipping...

Page 24: ...information Before installation ensure that your site is properly prepared The instrument should be installed on an optical table with vibration isolation that can carry a weight of at least 250 kg A...

Page 25: ...The switched on devices are registered and checked 4 2 Switching off the instrument 1 Close the ImSpector software 2 Shut down the Windows system 3 Switch off the camera 4 Switch off the instrument 5...

Page 26: ...ive window Live window Select left light sheet Activates the light sheet on the left hand side Settings 1 window Select both light sheets Activates the light sheets on both sides Settings 1 window Sel...

Page 27: ...agents Immediately replace the waste bottle after unmounting and fasten the bottle closure to the new bottle Wear personal protective equipment such as gloves safety glasses etc as indicated in the sa...

Page 28: ...2 Setting the parameters for the alignment 1 Start the ImSpector software 2 Select the appropriate Instrument Mode in the Measurement Wizard window for the ordered configuration such as Zoom Body 3 Se...

Page 29: ...lect the Measurement tab and select the imaging solution used in the cuvette under Liquid 8 Set the exposure time to a value between 100 and 200 ms in the Camera view 9 Select the objective lens such...

Page 30: ...5 PREPARING THE INSTRUMENT 28...

Page 31: ...ning the central light sheet on the left hand side in the z plane Later the screw can be used for the fine adjustment of the z shift between the left and right The screws Z2 and Z4 are used for aligni...

Page 32: ...aser beam Do not insert reflective objects into the cuvette Do not manipulate the alignment tool during alignment Before setting the light sheet path ensure that not any bubbles are present to the sid...

Page 33: ...arper the lines the better is the z position 5 Decrease the sheet width to the standard refer to Setting the parameters for the alignment on page 26 6 Lower the objective lens and focus on the light s...

Page 34: ...ofile 12 Right click on the dark gray part of the y axis and activate Grow This setting makes it easier to visualize the intensity of the light sheet 13 Use the knobs of the focusing unit l outer knob...

Page 35: ...ne passes the pinhole Ensure that the light sheet focus is always in the center and the Optics sheet NA is at the maximum If necessary readjust the z direction 3 If only one line passes the pinhole in...

Page 36: ...f interest over the light sheets passing the pinhole in the live window and activate the intensity profile 5 Check the adjustment by clicking the Brain button under Scan Range in the Settings 2 window...

Page 37: ...e center of the mask of the alignment tool 2 Adjust the height of the lateral light sheets Start with the rear light sheet using the adjustment screw Z2 Refer to Figure 6 1 Use an Allen key to align t...

Page 38: ...6 LASER ALIGNMENT 36...

Page 39: ...holder to the sample holder frame 3 Place the sample holder frame into the cuvette NOTICE Ensure not to drop any medium into the microscope chamber 7 2 Capturing a single color z stack 7 2 1 Aligning...

Page 40: ...the Settings 1 window and select the imaging solution used in the cuvette 8 Select the excitation and emission wavelengths for your sample under Filter for Measurement Channel Setup in software versi...

Page 41: ...ement settings 1 Draw a rectangle over an illuminated and or a dark region If drawing is not possible click the ROI for active profile button on the right side of the live window twice 2 Click the Adj...

Page 42: ...anged 7 2 3 Capturing an image 1 Move in the z direction to the starting position 2 Click Current Position Set as Zero in the Settings 2 window 3 Click the Take button under Scan Range Start 4 Move in...

Page 43: ...The selected checkboxes are used 5 Move the slider under Laser Transmission Control while the filter is highlighted to set the laser power for each individual filter NOTICE Higher laser power will ble...

Page 44: ...ion in the Measurement Wizard 2 Click the Mosaic tab in the xyz Table Visual XY window 3 Double click the green square representing the field of view The square will turn gray 4 Click and drag the bla...

Page 45: ...7 SETTING UP AN EXPERIMENT 43 5 Enter a percentage value to set an Overlap 6 To start the measurement proceed as described under Capturing an image on page 40...

Page 46: ...7 SETTING UP AN EXPERIMENT 44...

Page 47: ...table cleaning agents on a soft cloth to wipe the instrument 1 Unplug the instrument 2 To disinfect wipe accessible surfaces with 70 ethanol or isopropanol 3 If necessary wipe the instrument with a da...

Page 48: ...8 MAINTENANCE 46...

Page 49: ...m Weight 47 kg w o laser source Nominal input voltage 12 V DC provided by external power supply Working conditions Temperature 15 27 C Humidity max 70 relative humidity noncondensing Storage condition...

Page 50: ...43 mm Chromatic correction Dynamic 400 850 nm Sheet optics Illumination Uni and bidirectional Number of light sheets 1 6 Thickness 4 24 m Width 1 20 mm Numerical aperture 0 0135 0 135 Focus positionin...

Page 51: ...duct conforms to the requirements of the following directives 2006 42 EC Machinery 2014 30 EU Electromagnetic compatibility 2014 35 EU Low voltage In case of any change to the product not executed or...

Page 52: ...Supply of machinery Safety regulations 2008 Electromagnetic Compatibility Regulations 2016 Electrical Equipment Safety Regulations 2016 In case of any change to the product not executed or authorized...

Page 53: ...entative or Miltenyi Biotec Technical Support at Miltenyi Biotec headquarters Miltenyi Biotec B V Co KG Friedrich Ebert Stra e 68 51429 Bergisch Gladbach Germany Phone 49 2204 8306 3803 technicalsuppo...

Page 54: ...10 TECHNICAL SUPPORT 52...

Page 55: ...notice shall include the product model and serial number if applicable and details of the warranty claim and b return the nonconforming or defective product to Miltenyi Biotec freight prepaid only aft...

Page 56: ...T EXCEPT AS EXPRESSLY PROVIDED IN THIS WARRANTY STATEMENT MILTENYI BIOTEC HEREBY DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESS OR IMPLIED ORAL OR WRITTEN WITH RESPECT TO THE PRODUCT INCLUDING WITHOUT...

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Page 58: ...ris France Phone 33 1 56 98 16 16 macsfr miltenyi com Hong Kong Miltenyi Biotec Hong Kong Ltd Unit 301 Lakeside 1 No 8 Science Park West Avenue Hong Kong Science Park Pak Shek Kok New Territories Hong...