MOLECULAR DEVICES > CLONEPIX 2
ROBOT MANUAL
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14.
In a sterile environment, lift head out of sterilizing agent.
15.
Depress highest piston to allow interior to empty.
16.
Submerge head into a beaker of sterile water.
17.
Depress highest piston to allow interior to fill.
18.
Lift head out of water.
19.
Depress highest piston to allow interior to empty.
20.
Repeat steps 8-11 multiple times to remove Sterilizing Agent.
21.
Hold head over an empty beaker and spray liberally with 100% ethanol including
the interior.
22.
Depress highest piston and ensure that the interior is well-drained.
23.
Leave to air dry.
24.
Replace picking head.
Important note:
Autoclaving the head is not recommended as this can cause deterioration
of function. Molecular Devices recommends that each project/group should have at least one
picking head.
Picking Pins, O-rings and Pin Removal Key
The Picking Pins, O-rings and Pin Removal Key should be regularly cleaned and autoclaved
using the following procedure:
1.
Remove pins from picking head (see instructions below).
2.
Make a 2% solution (50mls) of aQu Clean Pin Cleaning Solution (K2505)
in a small plastic beaker.
3.
Place all the items in the solution and sonicate for 10 minutes.
4.
Rinse thoroughly in de-ionized water.
5.
Place items in de-ionized water and sonicate for 10 minutes.
6.
Autoclave pins, O-rings and pin removal key.
Recommended standard autoclave conditions are 121°C at 15 psi (103 kPa) for 20 minutes.
Molecular Devices recommends that each project/group should have at least two sets of
Picking Pins.
Picking Tubing
Picking Tubing (X4970) is the 8 silicon tubes that connect between the picking head and the
actuator. These tubes can be cleaned along with the picking head, or can be autoclaved using
standard conditions: 121°C at 15 psi (103 kPa) for 20 minutes.
Ethanol Wash Bath & Nylon Bristle Insert
The stainless steel Ethanol Wash Bath & Nylon Bristle Insert is filled with 70% ethanol in
normal use.
If contamination is suspected the Wash Bath and Bristle Insert may occasionally be
autoclaved using standard conditions: 121°C at 15 psi (103 kPa) for 20 minutes. Slight
distortion of the nylon block following autoclaving is normal, and this can be forced back to
straightness. However, a severely warped block must not be used as this may cause pin /
robot damage.
