Instrument Performance Factors
30
5000450 D
For a preview scan, the same 5
μ
m spots are scanned. However the
Y-direction step size is increased to 40
μ
m, and each 40
μ
m preview
scan pixel is an average of eight contiguous 5
μ
m spots in the
X-direction. This speeds image acquisition for balancing the PMT gains
while minimizing photobleaching.
Figure B-1 Data scans
.
is a representation of how the GenePix 4000B
Microarray Scanner samples 5
μ
m spots and averages them to calculate
the values of the image pixels for the various scan modes and pixel
sizes. On the left side of each image pair is the raw data scan from the
scanner. Each square represents a 5
μ
m by 5
μ
m spot as scanned by
the 5
μ
m laser beam. On the right are the resultant image pixels at
various resolutions. The shading indicates the spots that are scanned
as well as the corresponding pixels in the final image.
.
Figure B-1
Data scans
GenePix_4000B.book Page 30 Friday, October 22, 2010 3:07 PM
