Molecular Devices QPix 420 User Manual Download

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Chapter 7: Blue-White Picking Processes

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Selecting the Head and Sanitizing Options

To select the head and sanitizing options:

1. In the Head and Sanitise window, from the Picking Head list, select the head to use for the

picking routine.

2. In the Pin Columns (Rows) Before Inoculation list, select the number of pins to use to pick in

each column or row before transferring the picked colonies to the destination plate.
For the fastest transfer rate, select All to use all available picking pins to pick colonies
before inoculating the destination plate.
For smaller picking groups, the available selections depend on the Deposit Order selection
made in the Destination Options window.

If you select By Columns, you can pick colonies using from 1 to 11 columns.
If you select By Rows, you can pick colonies using from 1 to 7 rows.

To run the wash routine from the Sanitise profile after each partial pick and inoculation,
select the Wash Between Partial Inoculation check box. Clearing this check box waits until
all pins have been used for picking and inoculation before running the wash routine.

3. In the Destination field, enter the distance in millimeters above the bottom of the

destination plate wells to dip the pins for the inoculation.
If you select Make Copy for the destination plate, enter a value for the copy.

4. From the Sanitise Profile list, select the Sanitise profile to use for the picking routine.

If the available profiles are not suitable for the picking routine, exit the picking process and
create a new Sanitise profile. See

Creating and Editing Sanitise Profiles on page 33

5. Click Next to define options for the source receptacle.

Setting Source Receptacle Options

To set source receptacle options:

1. In the Source window, from the Holder list, select the type of holder used to hold the source

receptacles on the instrument deck.

2. From the Receptacle list, select the type of receptacle used for the source. Only the

receptacles applicable for the holder display in this list.
The preview image displays a representation of the holder and receptacle.

3. In the Positions field, enter the number of receptacles to use for picking.
4. In the Offset field, enter the position of the first receptacle to use for picking.

The preview image displays the defined locations of the receptacles.

Note: If you are using Validation barcodes, only one source receptacle shows, and
the Positions and Offset fields are not available.

5. In the Picking Depth Into Agar (mm) field, enter a value in millimeters to define the depth

that the pins are to be inserted in the agar for picking.

6. Select the Limit Max. Number of Features Per Position check box and then in the Max.

Number of Features Per Position field, enter the maximum number of colonies that can be
picked from a single QTray or Petri dish.

Note: Setting a limit for the maximum number of colonies in the Source window
prevents selecting a maximum number in the Feature Selection window when
running the picking routine. See

Selecting Colonies for Picking on page 53

7. Click Next to view a summary of the settings.