QPix 420 Colony Picking System User Guide
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Selecting the Head and Sanitizing Options
To select the head and sanitize options:
1. In the Head and Sanitise window, from the Gridding Head list, select the head to use for the
gridding routine.
2. From the Sanitise Profile list, select the Sanitise profile to use for the gridding routine.
If the available profiles are not suitable for the gridding routine, exit the gridding process
and create a new Sanitise profile. See
Creating and Editing Sanitise Profiles on page 33
.
3. Click Next to define options for the source and destination receptacles.
Setting Source and Destination Options
To select source and destination options:
1. In the Microplates and Filters window, from the Select Microplate list, select the plate type
for the source. If the plate type that you need is not listed, contact Molecular Devices to add
a new plate type to the list. See
2. Select either to dip the pins a number of times or to stir the wells of the source plate.
To dip the pins a number of times, click Multi Dip and in the Number of Dips field, enter
the number of times to dip the pins.
To stir the wells, click Stir Source.
3. In the Inoculation Height (mm) field, enter the distance in millimeters (mm) above the
bottom of the plate where the pins stop in the Source plate wells.
4. From the Destination Receptacle list, select Filter, OmniTray, or QTray.
5. Click Next to define the filter design layout.
Creating Filter Design Layouts
In the Filter Design window, you define the Spot Pattern to be stamped on your destination
surface in the defined Field Pattern.
The Spot Pattern determines the pattern and the number of times that a pin is stamped on
the destination surface. The numbers and colors represent the samples taken from
separate plates and stamped on the surface in the defined locations.
The Field Pattern divides the destination surface into areas, or fields, that are the size of a
96-pin head. Each field represents one head stamp of the defined Spot Pattern. The colors
and numbers identify the fields. Duplicate fields can be created where two or more fields
have the same number and color. The field number also influences the sample deposit
order. Field 1 is visited before field 2. Six fields are available for QTray and Filter destination
receptacles, while only one field is available for an OmniTray destination receptacle.
Summary of Contents for QPix 420
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