Molecular Devices QPix 420 User Manual Download

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Chapter 7: Blue-White Picking Processes

The QPix 420 Colony Picking System can pick colonies from receptacles using either standard
picking or regional picking. You can set up control wells for the destination plates before
running a picking process.

The following options are available in the Navigation window under Picking Processes:

Picking picks colonies from receptacles using the standard process. See

Picking Processes

on page 39

Blue-White Picking picks blue or white colonies from receptacles using the standard
process described in this chapter.
Zone of Inhibition Detection Picking picks colonies with detectable zones of inhibition from
receptacles using the standard process. See

Zone of Inhibition Detection Picking

Processes on page 83

Regional Picking picks colonies from receptacles using the regional process. See

Regional

Picking Processes on page 99

Blue-White Regional Picking picks blue or white colonies from receptacles using the
regional process. See

Blue-White Regional Picking Processes on page 119

Control Plate Creation creates a batch of plates containing control samples. These can be
created by picking colonies from source receptacles into destination wells. See

Control

Plate Creation Processes on page 139

Create New Regional Tray adds new plate source definitions to the regional picking plate
type database, which can then be selected for use in the regional picking process. See

Manage Regional Tray Processes on page 153

Note: Before running a picking process, it is important that you do the cleaning and set
up procedures in

Preparing to Run Processes on page 27

If this is your first picking process, you must edit or create a Sanitise profile to use with the
picking process. See

Creating and Editing Sanitise Profiles on page 33

Blue-White Colony Detection is a technique that uses the E. Coli LacZ gene to permit visual
detection of colonies that contain plasmids with DNA inserts. The Blue-White Picking function
must be used with a QPix® Chroma Filter. This filter is placed on the light bed of the instrument
under a QTray or a Petri dish holder containing the blue and white colonies to be detected.
QPix Chroma Filters are consumables that can be purchased individually and in packs of 25.
See

Replacement Parts and Optional Extras on page 215

CAUTION! When reusing a QPix Chroma Filter, use gentle handling, because crinkles
and bends can compromise image quality. Replace damaged filters.

This is a white light imaging picking processes only that requires the purchase of

QPix® Chroma

Colorimetric Colony Selection Software License Blue/White Colony Selection Software License

to function. If you would like to add this special license-enabled capability, contact your
Molecular Devices representative or technical support. See

Obtaining Support on page 209

Note: Due to inherent biological variations, some picked white colonies lose their
insertions and might later be viewed to be blue. These effects are inherent to the biology
and are not due to the picking or selection processes. When selecting the insertion
methodology, take care to make sure that the desired results are achieved.