Molecular Devices SPECTRAmax 340PC, Operator'S Manual

Page 1: ...SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale California 94089 Part 0112 0049 Rev A...

Page 2: ...Computer Incorporated All other company and product names are trademarks or registered trademarks of their respective owners Disclaimer Molecular Devices Corporation reserves the right to change its...

Page 3: ...on this product except to the extent required by applicable law The Seller specifically excludes all express and im plied warranties including without limitation any implied warranty that the product...

Page 4: ...iv SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 5: ...9 Templates 1 9 The Back Panel 1 10 Functional Description 1 11 Instrument Settings 1 11 Saving a Protocol 1 11 Recalling a Saved Protocol 1 11 Modes of Stand Alone Operation 1 12 Endpoint 1 12 Kineti...

Page 6: ...ate 3 6 Operation Overview 3 6 Chapter 4 Maintenance 4 1 Technical Support 4 3 Warnings and Cautions 4 3 General 4 4 Cleaning 4 4 Cleaning the Fan Filter 4 4 Changing the Fuses 4 5 Long Term Shutdown...

Page 7: ...f the SPECTRAmax 340PC 1 10 Figure 2 1 View of Rear Panel 2 4 Figure 3 1 Preparing the Blank Pattern Template 3 4 Figure 4 1 Underside of SPECTRAmax 340PC 4 5 Figure 4 2 Pry Open the Fuse Box Cover 4...

Page 8: ...person working with the system Indicates a condition involving potentially infectious biologi cal agents requiring that proper handling precautions be taken Glossary of Terms Absorbance A The amount o...

Page 9: ...mplates 1 9 The Back Panel 1 10 Functional Description 1 11 Instrument Settings 1 11 Saving a Protocol 1 11 Recalling a Saved Protocol 1 11 Modes of Stand Alone Operation 1 12 Endpoint 1 12 Kinetic 1...

Page 10: ...Chapter 1 1 2 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 11: ...es released from cells and kinetic ELISAs Standard 96 well microplates strip wells and filter bottom microplates can be used in the SPECTRAmax 340PC The contents of the wells in a microplate can be mi...

Page 12: ...conventional basis for quantifying the unique absorptivity properties of compounds in solution Quantitative analyses can be performed on the basis of extinction coefficients without standard curves e...

Page 13: ...on of the SPEC TRAmax 340PC The control panel can be used to configure the instrument set tings store and recall assay protocols and to initiate readings When you press a control panel key the SPECTRA...

Page 14: ...h 9 0 is reserved for the default instrument proto col Recall Recalls the instrument settings previously stored using the Save key 1 nm Selects the measurement wavelength Pressing the up or down arrow...

Page 15: ...ou increment the setting to the highest limit 45 C and continue to press the up arrow the display will not change If you decrement the setting to the lowest limit 15 C and continue to press the down a...

Page 16: ...roplate drawer to close automatically if it was open after which the selected assay read mode begins Status Indicators At the far right of the LCD are indicators as shown in Figure 1 3 These indica to...

Page 17: ...tomatically After the read the drawer will open for about 10 sec onds allowing you to remove the plate During a kinetic read in either stand alone mode or under computer control the drawer can be open...

Page 18: ...Printer port double shielded 25 pin parallel for use in stand alone opera tion plug the 25 pin end of the cable into this port the other Centronics end attaches to a port on the printer Computer port...

Page 19: ...ol in memory first define the instrument settings for the proto col by setting the parameters as desired Then press the up or down arrows on the Setup key until the desired number under which to save...

Page 20: ...many advantages when determining the relative activity of an enzyme in different types of microplate assays including ELISAs and the purifi cation and characterization of enzymes and enzyme conjugates...

Page 21: ...ctory presets or by reading an empty drawer in the BLANK mode Each time a microplate is read the average OD or milli OD minute reading of the wells in the current blank pattern will be computed This m...

Page 22: ...dual wavelength Temperature Regulation The SPECTRAmax 340PC has been designed to regulate the temperature of the microplate chamber from 4 C above ambient to 45 C Upon power up when the incubator is o...

Page 23: ...ompare the measured air temperature inside the chamber against the tempera ture set point and use the difference to calculate the heating cycles This tech nique results in accurate precise control of...

Page 24: ...s from the first reading are being printed If you have performed a blank reading the blank values will be sub tracted from raw OD values and the calculated result will be shown on the printout Default...

Page 25: ...ator on or off to control the temperature in the microplate chamber Use PathCheck to normalize the absorbance readings in each microplate well to a 1 cm pathlength Use the Automix function to shake th...

Page 26: ...maximum kinetic rates on non linear data Assign plate group or sample blanks Perform pathlength correction based on 1 cm cuvette readings Customize data analysis for each group in the template Create...

Page 27: ...nearity 405 nm 0 000 OD to 3 000 OD Photometric accuracy microplate 0 2 0 OD 340 850 nm 1 0 and 0 006 OD 2 0 2 5 OD 340 850 nm 3 0 and 0 006 OD Photometric precision repeatability 0 2 0 OD 340 850 nm...

Page 28: ...en temperature regulation en abled The ambient temperature must be 20 C to achieve temperature regulation at 45 C Resolution 0 1 C Accuracy 1 0 C Well to well uniformity at equilibrium 0 5 C at 37 C C...

Page 29: ...fer Memory downloading of data buffer 100 plate maximum Computer interface 8 pin DIN RS 232 serial double shielding required Printer interface Parallel 25 pin to Centronics double shielding required M...

Page 30: ...Chapter 1 1 22 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 31: ...Chapter 2 Installation Installation Warnings 2 3 Installation Cautions 2 3 Unpacking 2 3 Setting Up for Stand Alone Use 2 4 Setting Up for Computer Control 2 4...

Page 32: ...Chapter 2 2 2 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 33: ...n the carton and the packing materials If the unit should need to be returned for repair you must use the original packing materials and carton for shipping If the carton has been damaged in transit i...

Page 34: ...hat you use a surge protector between the power cord and the grounded power outlet 6 Turn the SPECTRAmax 340PC around so that the control panel now faces you Be sure no cables run beneath the instrume...

Page 35: ...ded power outlet of the appropriate voltage Molecular Devices recommends that you use a surge protector between the power cord and the grounded power outlet 6 Turn the SPECTRAmax 340PC around so that...

Page 36: ...Chapter 2 2 6 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 37: ...Turn the Instrument and Printer On 3 3 Set the Temperature 3 3 Choose a Read Mode 3 3 Using a Blank Pattern 3 3 Endpoint Mode 3 4 Kinetic Mode 3 5 Select the Wavelength s 3 5 Save Recall Instrument Se...

Page 38: ...Chapter 3 3 2 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 39: ...int readings where temperature control is not required and for ambient Kinetic assays To enable the incubator press the incubator On Off key located below the Temp C key An indicator at the right side...

Page 40: ...1 making sure the template is flush on all sides and lies flat Touch the READ key any time after the template is properly positioned in the drawer When reading is complete the instrument will print a...

Page 41: ...mode by pressing the On Off key below the 1 2 nm key on the control panel The LCD will show the currently selected reference 2 wavelength Change the wavelength by pressing the arrows on the 1 2 nm ke...

Page 42: ...n two seconds four sec onds if dual wavelength has been selected send a header for the reading to the printer close the drawer if it was open and read the microplate according to the selected instrume...

Page 43: ...nk pattern prior to reading the microplate darken the well areas corre sponding to the blank wells using a blanking template or fill the same wells of a microplate with anything having an OD greater t...

Page 44: ...Chapter 3 3 8 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 45: ...Chapter 4 Maintenance Technical Support 4 3 Warnings and Cautions 4 3 General 4 4 Cleaning 4 4 Cleaning the Fan Filter 4 4 Changing the Fuses 4 5 Long Term Shutdown 4 7 Moving the SPECTRAmax 340PC 4 7...

Page 46: ...Chapter 4 4 2 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 47: ...ar Devices representative Removal of protective covers that are marked with the High Voltage warning symbol shown below can result in a safety hazard Always turn the instrument power switch off and re...

Page 48: ...cted to a tray at the bottom of the instrument from which they will exit to the bench or counter beneath the instrument Wipe up any spills immediately Clean only the exterior of the unit and the micro...

Page 49: ...of the SPECTRAmax 340PC If power failed while the SPECTRAmax 340PC was already on check that the power cord is not loose or disconnected and that power to the power outlet is functioning properly If t...

Page 50: ...to the rear panel 4 On the left hand side of the rear panel viewed from the back is the power cord receptacle As shown in Figure 4 2 insert a small flat blade screwdriver into the slot behind the ton...

Page 51: ...instrument and to the wall outlet and reconnect other cables previously disconnected Long Term Shutdown If you will not be using the SPECTRAmax 340PC for an extended period of time clean the external...

Page 52: ...Chapter 4 4 8 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 53: ...Chapter 5 Troubleshooting Error Codes and Probable Causes 5 3 Error Messages 5 3 Error Code Classifications 5 3 Warning Messages 5 5 Opening the Drawer Manually 5 5...

Page 54: ...Chapter 5 5 2 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 55: ...ges do not stop a reading but are logged in the error buffer Error Code Classifications Not all error messages are listed in this manual The errors are grouped in rela tionship to possible causes as f...

Page 56: ...ther task Example Request a wavelength while the monochromator is in motion 107 command error measure ment in progress Instrument could not perform the command because a measure ment was in progress 1...

Page 57: ...losed turn the power off and using your thumbnail locate the groove in the upper left side wall of the door Open the door and use your index finger to pull the microplate drawer out of the instrument...

Page 58: ...Chapter 5 5 6 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 59: ...requirement Please contact your local Molecular Devices representative for information regarding recommended printers Cables Molecular Devices recommends that you use high quality dou ble shielded cab...

Page 60: ...Appendix A A 2 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 61: ...e 0200 2405 Cable RS 232 Macintosh to instrument 8 pin DIN 8 pin DIN 9000 0091 Cable RS 232 PC to instrument 9 pin 8 pin DIN 9000 0149 Cable parallel printer 25 pin Centronics 9000 0145 Power Cord 440...

Page 62: ...Appendix B B 2 SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 63: ...llection 1 16 printed output 1 16 Display 1 5 Drawer 1 8 1 9 3 4 open manually 5 5 Dual wavelength 3 5 E ELISA 1 3 Endpoint 1 12 3 4 Error messages 5 3 F Fan filter 4 4 Fuses 4 5 accessing 1 10 I Incu...

Page 64: ...te of reaction 1 12 Read 1 7 cycle 1 12 mode 1 7 1 8 3 3 3 4 3 6 READ key 1 8 Read mode 1 7 3 6 Remote control 1 5 1 17 Run time Kinetic 1 7 3 5 S Secondary functions 1 7 Set point 1 7 Setup 1 6 Shift...

Page 65: ...Drawer 1 9 Microplates 1 9 Templates 1 9 The Back Panel 1 10 Functional Description 1 11 Instrument Settings 1 11 Saving a Protocol 1 11 Recalling a Saved Protocol 1 11 Modes of Stand Alone Operation...

Page 66: ...croplate 3 6 Operation Overview 3 6 Chapter 4 Maintenance 4 1 Technical Support 4 3 Warnings and Cautions 4 3 General 4 4 Cleaning 4 4 Cleaning the Fan Filter 4 4 Changing the Fuses 4 5 Long Term Shut...

Page 67: ...er 1 9 Figure 1 5 Components on the Back Panel of the SPECTRAmax 340PC 1 10 Figure 2 1 View of Rear Panel 2 4 Figure 3 1 Preparing the Blank Pattern Template 3 4 Figure 4 1 Underside of SPECTRAmax 340...

Page 68: ...ii SPECTRAmax 340PC Microplate Spectrophotometer Operator s Manual...

Page 69: ...PECTRAmax 340PC Microplate Spectrophotometer Operator s Manual iii Table 1 1 Intervals and Number of Readings for Total Kinetic Run Time1 13 Table 5 1 Error Codes Messages and Notes about the Errors5...