Motic AE2000 BIN, Instruction Manual

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Page 2: ...uirements of modern research techniques and testing methods This involves modification to the mechanical structure and optical design of our instruments Therefore all descriptions and illustrations in this instruction manual including all specifications are subject to change without notice ...

Page 3: ...Mounting the Condenser 13 4 Installing the Objectives 13 5 Mechanical Stage 14 6 Inserting the Eyepieces 15 IV Microscope Handling 16 1 Interpupillary Distance Adjustment 16 2 Diopter Adjustment 16 3 Coarse and fine focusing 17 4 Coarse focus torque adjustment 17 5 Changing between Halogen and LED illumination 18 6 Brightfield Microscopy 19 7 Phase Contrast Microscopy 19 8 Filter selection 22 9 Au...

Page 4: ...bleshooting Table 24 1 Optical and Operating Problems 24 2 Electrical 24 VII Care and Maintenance 25 1 Lenses and Filters 25 2 Cleaning of Painted or Plastic Components 25 3 When Not in Use 25 4 Warning Label 26 ...

Page 5: ...strument for living cells observation and other microbiological samples Due to our Colour Corrected Infinity Optics CCIS the AE2000 offers superior image quality combined with excellent mechanical performance Daily work is facilitated by a number of ergonomic features 2 Nomenclature Model AE2000 ...

Page 6: ...5 Model AE2000 ...

Page 7: ...6 Model AE2000 TRI Model AE2000 Ergo ...

Page 8: ... 239mm Stage height 192mm Object guide Verniers with numerical and alphabetic scale X direction numerical scale readable from right to left Y direction alphabetic scale Objectives Plan Achromat 4x N A 0 1 W D 12 6mm Plan Achromat 10x N A 0 25 W D 16 8mm LWD Plan Achromat 20x N A 0 3 W D 4 7mm LWD Plan Achromat 40x N A 0 5 W D 3mm Plan Achromat PH 4x Ph0 N A 0 1 W D 12 6mm Plan Achromat PH 10x Ph1 ...

Page 9: ...degree 2 in according with IEC60664 y Installation Overvoltage category 2 in according with IEC60664 y Air Pressure of 75kPa to 106 kPa 2 Input voltage and power Automatic voltage selection works with electrical outlets worldwide It is advised to always use a power cord that is rated for the voltage used in your area and that has been approved to meet local safety standards Using the wrong power c...

Page 10: ...d to unplug the instrument when not in use This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 this device must accept any interference received including interference that may cause undesired operation ...

Page 11: ...to prevent electric shock always turn the power switch off Fig 1 and unplug the power cord Fig 2 before replacing the bulb y To remove lamphouse cover press down lightly 1 and rotate counter clockwise 2 Fig 3 Power Switch Fig 1 Power Cord Fig 2 Lamp house Cover Fig 3 ...

Page 12: ...d by bulb If surface is contaminated wipe it clean using lens tissue or soft cotton Fig 6 Fig 7 LED module y Firmly insert the LED module into the socket pinholes until it reaches the limit This is a Motic patent design to exchange LED module and halogen bulb on the same socket directly y After the LED module installation Fig 6 secure it with the clamp screw by 1 5mm hexagonal screwdriver supplied...

Page 13: ... maintains the same level of brightness and colour temperature regardless of whether it is new or nearing the end of its life span 1 2 LED module The LED module is specially designed to be inserted into halogen bulb socket directly converting halogen illumination to LED illumination LED is more economical and environmental friendly and combines the advantages of low heat and long life span 2 Filte...

Page 14: ...ig 12 Fig 11 Fig 12 y If Phase contrast is to be used insert the Phase slider into the slot of the condenser The centering screws of Phase slider have to face the front side Fig 13 Fig 13 4 Installing the Objectives y Remove the stage insert from the stage Fig 14 y Screw in the objectives in the nosepiece thread holes in a manner that the magnification increases with clockwise rotation of the revo...

Page 15: ...ecise X Y movement of common vessels for cell culture For choosing the respective vessel carrier please contact your local Motic supplier y Secure the mechanical stage to the AE2000 plain stage using the two mounting screws Fig 16 located beneath the stage on the right side user facing the front of the instrument Fig 16 ...

Page 16: ...from the eyepiece tubes y Use the same magnification eyepieces for both the eyes y Insert each eyepiece into the eyepiece sleeve by a twisting movement Fig 17 y Should the rubber eye guards are to be used fit them in the groove around the eyepiece Fig 17 ...

Page 17: ...o reduces the extent to which focusing is lost when the objective magnification is changed In particular this occurs when a low magnification objective is used y Set the diopter on both eyepieces to the 0 position y Swing in the 10x objective focus with one eye only as everybody has got a strong eye use this one for this first focussing Fig 18 Fig 19 y When in focus close this eye and use the othe...

Page 18: ...he fine focus knob equals 2 microns Please avoid following action y Never attempt either of the following actions since doing so will damage the focusing mechanism y Rotate the left or right knob while holding the other y Turning the coarse and fine focus knobs further than their limit 4 Coarse focus torque adjustment y To increase the torque turn the torque adjustment ring located behind the left...

Page 19: ...Installing the new bulb into the socket pinholes until it reaches the limited be careful not to tilt it when mounting y If installing the halogen bulb do not touch the glass surface of the lamp with bare fingers Doing so will cause fingerprints grease etc to burn onto the lamp surface reducing the illumination provided by the bulb If surface is contaminated wipe it clean using lens or soft cotton ...

Page 20: ... but increase the contrast and depth of focus An image with appropriate contrast in most cases can be obtained with an aperture diaphragm closed down to 2 3 Fig 24 7 Phase Contrast Microscopy y Phase contrast objectives are labelled Ph Ph0 Ph1 Ph2 y For using phase contrast be sure to use the annular ring in the slider has the same phase label as the objective Ph0 Objective 4x Ph1 Objective 10x 20...

Page 21: ...lumination annulus do not coincide use the two allen keys 1 5mm supplied with the microscope Fig 26 Insert the keys into the countersunk screws Turn the keys to move the illumination annulus in the slider until the image of the illumination annulus is concentric with the phase plate Fig 27 y This complete procedure has to be performed for the respective phase lens illumination ring combinations y ...

Page 22: ...er carrier y To insert replace an annular diaphragm take care the diaphragm has got the correct orientation Fig 28 Push the diaphragm against the flexible filament until it fits in the hole Fig 29 If necessary use the allen keys to bring the annulus to the center of the hole A fine adjustment will be done by using the centering telescope see above Fig 28 Fig 29 ...

Page 23: ...crography 9 Auto Off On switch function Fig 30 y The power control knob is located above the left focusing knob y When auto is selected the blue pilot lamp indicates that an IR sensor is activated Fig 30 If no user is detected in front of the microscope the microscope illumination will automatically switch off after 15 minutes When the user returns the illumination will start again Never attempt t...

Page 24: ...activated 80 of the light will enter the camera y Select a blue filter for routine application An additional colour compensating filter can also be used depending on the colour rendition y A change of depth of focus contrast and resolution of image is attainable with an aperture setting of about 2 3 maximum Fine setting of the condenser aperture is depending on the individual sample y For photomic...

Page 25: ... into position Mod AE2000 trinocular only Dust or dirt in field of view Aperture diaphragm closed too far Dust or dirt on specimen s surface Inadequate image quality Brightfield objective used with Phase illumination Annular ring does not fit to objective phase ring Annular ring has to be readjusted Eye strain or fatigue Interpupillary distance not adjusted Diopter adjustment not made Inadequate i...

Page 26: ...en flame y Do not use same area of gauze or lens tissue to wipe more than once 2 Cleaning of Painted or Plastic Components y Do not use organic solvents thinners alcohol ether etc Doing so could result in discolouration or in the peeling of paint y For stubborn dirt moisten a piece of gauze with diluted detergent and wipe clean y For plastic components only moisten a piece of gauze with water and ...

Page 27: ... the safest possible manner Warning Label Symbol Explanation Indicates that the surface becomes hot and should not be touched with bare hands CAUTION Risk of danger See user manual Proper handling of the microscope will ensure years of trouble free service If repair become necessary please contact your Motic agency or our Technical Service directly ...