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IMPORTANT MICROSCOPY TERMS

USING YOUR SILVERLINE MICROSCOPE

NUMERICAL APERTURE (NA)

A measure of an objective’s light gathering capabilities. The concept may be compared to the 

F-valve in photographic lenses. Generally speaking, N.A. values of less than 1.00 are "Dry" 

objectives. Values of 1.00 or greater require oil as a medium. Please note that condensers 

are part of the optical system and are also assigned an N.A. value. That value must be at 

least as high as that of the highest objective used

PARFOCAL

A term applied to objectives and eyepieces when practically no change in focus is needed 

when changing objectives. The objectives on your SILVER3H microscope are parfocalled at 

the factory so that only a slight adjustment of the fine focus knob is needed to maintain focus 

when switching magnification.

RESOLUTION OR RESOLVING POWER

The ability of a lens to define the details of the specimen at a maximum magnification. This 

is governed by the N.A. (Numerical Aperture) of the lens. For example, a 40X objective with 

a N.A. of 0.65 has a maximum resolving power of 650X, equal to 1000 times the N.A.. This 

rule of N.A. x 1000 is true of all achromatic objectives. 

WORKING DISTANCE

The distance from the lens of the objective to the cover slip on the slide, when the specimen 

is in focus.

CORDLESS OPERATION

The rechargeable battery should be fully charged for approximately 8 hours before the initial 

use. It can be charged by using the 4.5 Volt A/C adapter included with the microscope. An 

LED indicator light on the A/C adapter will be red while the battery is charging and will turn 

green when the battery is fully charged.

The battery can be used to power the illumination system for approximately 40 hours. If the 

microscope is used in the same location, the A/C adapter can remain plugged-in without 

damage to the battery or recharging system. 

SILVER

3H

 

SERIES

 

|  07 

  

SILVER

 LINE

Summary of Contents for SILVER3H Series

Page 1: ...SILVER3H SERIES INSTRUCTION MANUAL ENGLISH SILVER LINE...

Page 2: ...can be dismounted either to attach it on a standard camera tripod or to take it with you on a field trip It is cordless using energy efficient rechargeable LED illumination made of metal and compact s...

Page 3: ...ger outer knob of the focus control which facilitates rapid and heavy movement of the focusing mechanism In order to prevent gear damage the focus control is equipped with an upper limit stop that pro...

Page 4: ...or both transmitted bottom and incident top light the illumination system may be used while the SILVER3H is charging IRIS DIAPHRAGM The iris diaphragm is a round device that is mounted below the stage...

Page 5: ...X SIEDENTOPF SILVER3H B only A binocular head design where the interpupillary adjustment increasing or decreasing the distance between the eyepieces is achieved by pivoting the eyepiece tubes in an up...

Page 6: ...stinct image above and below the focal plane Depth of focus decreases with the increase of numerical aperture or with the increase of magnification EYE POINT OR EYE RELIEF The distance from the eyepie...

Page 7: ...The ability of a lens to define the details of the specimen at a maximum magnification This is governed by the N A Numerical Aperture of the lens For example a 40X objective with a N A of 0 65 has a...

Page 8: ...roscopic stage with a built in 0 65 N A condenser iris diaphragm stage clips and swing out white filter STAGE POSITION ADJUSTMENT Proper stage height is critical for achieving the correct focusing dis...

Page 9: ...on the stage securing it with the stage clips Center the specimen in the optical path 6 After securing and moving the slide into position rotate the nosepiece to place the lowest power 4X objective in...

Page 10: ...he same as for the 40X objective since the angle of the required light is determined by the numerical aperture N A of the objective The proper aperture of the diaphragm can be easily achieved after mi...

Page 11: ...into focus The specimen may require some centering in the field of view at this time By using the fine focusing knob slowly and carefully refine the focus to clearly observe the fine details of the sp...

Page 12: ...he eyepiece to moisten the lens and wiping dry with a clean lens tissue is sufficient to clean the surface Lenses should never be wiped while dry as this will scratch or otherwise mar the surface of t...

Page 13: ...outine maintenance light bulbs power supplies rechargers batteries fuses cords add on accessories damage resulting from repair by unauthorized parties accident alteration shipping misuse or abuse is n...

Page 14: ...882 2792 Motic Europe S L U Spain Pol gon Industrial Les Corts C Les Corts 12 08349 Cabrera de Mar Barcelona Spain Tel 34 93 756 6286 Fax 34 93 756 6287 CCIS is a trademark of Motic Incorporation Ltd...

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