MSD MESO SECTOR S 600, User Manual

Page 1: ...MESO SECTOR S 600 www mesoscale com USER MANUAL ...

Page 2: ...UAL Changes in Publication The information contained in this Manual is subject to change without notice Unauthorized Use of Manual Material No part of this Manual may be duplicated reproduced stored in a retrieval system translated transcribed or transmitted in any form or by any means without the express prior written permission of MSD This Manual shall be returned to MSD within two 2 business da...

Page 3: ...icing 18 3 System Description 20 3 1 Intended Use 20 3 2 Specifications Overview 20 3 3 Plate Compatibility 20 3 4 System Components 20 3 5 The SECTOR S 600 Instrument 21 3 5 1 CCD Camera and Telecentric Lens 22 3 5 2 Motion Control System 22 3 5 3 Plate Barcode Readers 22 3 5 4 MULTI ARRAY Plate Input Output Stacker 23 3 5 5 Stack Tubes 23 3 5 6 Single Plate Adapter 25 3 5 7 Stacker Cover Plate 2...

Page 4: ...k Tube Cleaning 49 7 3 Instrument Decontamination 49 8 Appendix 51 8 1 Troubleshooting Guide 51 8 2 Specifications 54 8 2 1 SECTOR S 600 54 8 2 2 Scientific Performance 54 8 2 3 Environmental Specifications 54 8 2 4 Power Requirements 54 8 2 5 SECTOR S 600 Physical Dimensions 55 8 2 6 Plate Standard Read Volume per Well 55 8 2 7 Custom Bar Code Compatibility 55 8 2 8 Plate Specifications 55 9 Tech...

Page 5: ...monstration Plate 31 Figure 3 13 Loading a demonstration plate 32 Figure 5 1 Instrument Status window 36 Figure 5 2 MSD DISCOVERY WORKBENCH desktop icon 37 Figure 5 3 SECTOR S 600 icon 37 Figure 5 4 MSD DISCOVERY WORKBENCH splash screen 38 Figure 5 5 SECTOR S 600 Window Setup Plate Summary and Operations areas 39 Figure 5 6 Stacker cover over the output port with empty single plate adapter in the ...

Page 6: ...6 1 Introduction ...

Page 7: ...SPOT plates measure multiple analytes simultaneously in a single well increasing throughput and enabling novel assay panels For more details and information about applications please visit the MSD website at www mesoscale com 1 1 Intended Audience This manual is for all users of the MESO SECTOR S 600 instrument Users should understand general computer and Microsoft Windows terminology and be famil...

Page 8: ...d understand and follow the instructions in this publication may result in damage to the product injury to operating personnel or poor instrument performance 1 4 Formatting Information This guide uses the following formatting conventions Internal hyperlinks are formatted bold gray Click to jump instantly to the referenced section or figure External hyperlinks are formatted underlined blue Click to...

Page 9: ...9 2 SafetyInformation ...

Page 10: ...n against harmful interference when the equipment is operated in a commercial environment The equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause interference in which case the user will be required to correct the interference at his own expense Regarding Industry Canada Interference Causing Equipment Sta...

Page 11: ... 2 Symbols and Labels Table 2 2 defines the symbols found in this manual on the instrument and on the instrument labels Table 2 2 Symbols and labels Symbol Label Description WARNING Warning messages are highlighted with this symbol and the word WARNING in red They advise operators of potential mechanical or other hazards and highlight the procedures or information necessary to avoid personal injur...

Page 12: ...ve The WEEE symbol above a horizontal bar indicates this product was placed on the market after 2005 in compliance with European Union EU directive 2012 19 EU the Waste Electrical and Electronic Equipment WEEE Directive The SGS System Certification Mark or Q mark is issued by the Société Générale de Surveillance It indicates this instrument has been tested by an accredited certification body for e...

Page 13: ...e avoided See Section 8 2 3 Environmental Specifications for complete environmental specifications CAUTION Keep the SECTOR S 600 away from direct sources of heat or cold and direct sunlight Ensure that the rear cooling vents and the CCD camera tower cooling vents on the SECTOR S 600 are not blocked CAUTION Do not place any objects materials or liquid containers on top of the SECTOR S 600 CAUTION F...

Page 14: ... parts For significant spills or liquid intrusion into the instrument enclosure e g resulting from a fire protection water sprinkler contact MSD Instrument Service WARNING The SECTOR S 600 has not been tested regarding intrinsic safety Accordingly the SECTOR S 600 must not be operated in hazardous classified atmospheres as defined by the National Fire Protection Association and the National Electr...

Page 15: ...acility when handling any of these reagents WARNING Samples user reagents or controls used in assays may be infectious or biohazardous By working with these materials users may be exposed to biological hazards Laboratory regulations concerning the handling of potentially infectious material should be followed at all times WARNING Labels are affixed to the stack tube platforms indicating a potentia...

Page 16: ...se regions while the instrument is powered up CAUTION When using robotic plate loaders robots ensure that there is nothing present that will obstruct robot movement WARNING A stacker cover plate should always be in place when a stack tube or single plate adapter is not present The stacker cover plate protects users from moving parts and protects the unused stacker area from dust and debris WARNING...

Page 17: ...oft Windows Update service can affect the operation of the instrument software When running the update application can turn off the database services upon which the instrument relies causing errors and stopping instrument operation Updates should not be run during instrument use Run manually or schedule Windows and other software updates when you are sure that the instrument will not be in use Occ...

Page 18: ... 600 instrument If shipping to MSD contact MSD Instrument Service to determine the level of decontamination required WARNING Follow site safety procedures and the directions of the site safety officer to determine decontamination requirements for the imager before shipping or service WARNING Prior authorization must be obtained before the instrument and or its accessories are shipped to MSD Author...

Page 19: ...19 3 SystemDescription ...

Page 20: ...c controller 3 3 Plate Compatibility The SECTOR S 600 is compatible with 96 and 384 well single spot and MULTI SPOT SECTOR plates 4 7 and 10 spot plates MSD plates are designed to be read only once and may not be reused 3 4 System Components A standard SECTOR S 600 system consists of the following Figure 3 1 SECTOR S 600 instrument Microsoft Windows compatible computer workstation MSD DISCOVERY WO...

Page 21: ...rcode readers short and long side Integrated MULTI ARRAY plate input output stack MULTI ARRAY plate Stack Tubes 2 standard capacity not shown Single Plate Adapter 1 not shown One Stacker Cover Plate 1 not shown Instrument status LEDs Power switch and I O panel not shown Motion Halt button on some configurations Additional stack tubes single plate adapters and stacker cover plates may be purchased ...

Page 22: ...00 uses a precision mechanism for transporting MULTI ARRAY plates from the input stacker into the CCD camera s viewing area and back to either the input or output stacker depending on selections in the DISCOVERY WORKBENCH Software See Section 6 Using the SECTOR S 600 for more information on reading a plate 3 5 3 Plate Barcode Readers The SECTOR S 600 instrument s two barcode readers read the barco...

Page 23: ...ates the input right stacker interface plate can act as both input and output ports For safety place a stacker cover plate over the left stacker interface plate when not in use WARNING The stacker contains components that may move at any time Users of the SECTOR S 600 should never place their fingers into the stacker for any reason unless the instrument is powered off 3 5 5 Stack Tubes Stack Tubes...

Page 24: ...System Description 24 Figure 3 4 Input right stacker interface plate Figure 3 5 Fully loaded standard and high capacity stack tubes ...

Page 25: ...e adapter also allows use of the instrument with laboratory robotic systems 3 5 7 Stacker Cover Plate The stacker cover plate Figure 3 6 left side of image is used to cover the output left stacker interface when the machine is set to return single plates to the input side Figure 3 6 SECTOR plate in single plate adapter WARNING A stacker cover plate should always be in place when a stack tube or si...

Page 26: ...er indicate the current operational mode of the SECTOR S 600 Figure 3 7 Steady blue Instrument is in standby mode Blinking blue Instrument is currently reading a plate Steady red Instrument is in fault mode or is waiting for the addition of plates Figure 3 7 SECTOR S 600 status LEDs ...

Page 27: ...h Typically the instrument should be left powered on to maintain camera temperature NOTE Only use the communications cables supplied with the SECTOR S 600 Use of cables other than those supplied may degrade instrument performance A halt connector is present in order to add an external switch that will de energize the motion control system The SECTOR S 600 connector receptacle is Hirose Electric Co...

Page 28: ...de energizes the main motion control components in the instrument However the remaining electronic components of the instrument remain powered and still pose a shock hazard WARNING The halt switch should never be obstructed or restricted from access The halt function can also be accessed through the rear I O panel to enable external safety devices to halt the motion of the SECTOR S 600 Please cont...

Page 29: ...gineer I O connections on the associated computer workstation are shown in Figure 3 10 Figure 3 10 Cable attachment locations for SECTOR S 600 3 7 MSD DISCOVERY WORKBENCH Software DISCOVERY WORKBENCH is a Windows application that supports the operation of the SECTOR S 600 instrument stores both current and historical plate data and analyzes and presents results The application has several componen...

Page 30: ...y Figure 3 6 The two position single plate handling mode uses a single plate adapter on the input side for loading and a second single plate adapter if purchased or stack tube on the output side for unloading The single plate loading and stack unloading mode uses the single plate adapter on the input side for loading and a stack tube on the output side for unloading See Section 6 3 Robotics Integr...

Page 31: ...OR S 600 has six separate sectors simulating the structure of MSD MULTI ARRAY plates Figure 3 12 as read by the SECTOR S 600 Figure 3 12 MESO SECTOR Demonstration Plate For the SECTOR S 600 sectors 3 and 4 Figure 3 11 of the demonstration plate have LEDs that test the CCD camera in the instrument The remaining four sectors of the plate contain known electronic components for testing the electrical...

Page 32: ...y UPS to ensure the integrity of data in the event of power line transients The UPS required will depend on the standard operating voltage at your laboratory s location Please ensure that the proper UPS is employed for the power conditions in your country and local area CAUTION To avoid interference from voltage transients the computer and instrument should be powered by the same electrical circui...

Page 33: ...33 4 Installation ...

Page 34: ... of the computer environment could interfere with proper operation of the instrument software CAUTION Running screen savers maintenance software network security software and possibly other software on the SECTOR S 600 computer could cause conflicts with the operation of the instrument software CAUTION Use of other applications while plates are being read may interfere with system performance Use ...

Page 35: ...35 5 QuickStart ...

Page 36: ...ate CCD camera cooling If the instrument has been off allow at least 45 to 60 minutes for the CCD camera to cool when restarting Operating temperature of 35 1 C must be reached before the DISCOVERY WORKBENCH software will allow the processing of any plates The Status window displays the temperature of the CCD camera while it is cooling Figure 5 1 Once the CCD camera has reached its operating tempe...

Page 37: ... name and MsdAdmin as the password This is the default login shipped with the system you may change this login password or add additional Windows user accounts 3 If DISCOVERY WORKBENCH does not start automatically double click the MSD DISCOVERY WORKBENCH icon on the Windows desktop Figure 5 2 Figure 5 2 MSD DISCOVERY WORKBENCH desktop icon 4 Click the instrument icon Figure 5 3 on the toolbar to c...

Page 38: ...menu 2 Select the Return Plates to Input Stack checkbox If left unchecked the demonstration plate will be placed in the output stack on the left side of the SECTOR S 600 after reading Because the demonstration plate will be run as a single plate it is most convenient to return it to the input stack after processing NOTE It is very important to select Return Plates to Input Stack checkbox when the ...

Page 39: ...the text field Figure 5 5 SECTOR S 600 Window Setup Plate Summary and Operations areas 5 Place the single plate adapter on the input right stacker interface plate Figure 5 6 Figure 5 6 Stacker cover over the output port with empty single plate adapter in the input port ...

Page 40: ...ready Click Run in the SECTOR S 600 window The Run Options dialog window will open Figure 5 8 1 Verify Setup Selections If changes are necessary click Cancel make changes in the area 5 2 Setup of the SECTOR S 600 window and re verify Setup Selections 2 Run name is optional but one can be entered in the second section 3 Verify the Export information and make changes if necessary Refer to the DISCOV...

Page 41: ...ent Eject Transfers a plate from inside the instrument but not being read to the stacker output port where it may be retrieved This function is disabled during a plate run Retract Lowers the stacker mechanism if needed and then ensures the instrument door is closed Any plates in the input location will remain where they are No further action is taken without input from the operator This function i...

Page 42: ...splay numbers above 2000 and dark wells should display numbers below 200 Figure 5 9 shows a Color Map view of the data from the demonstration plate The values shown in this figure will differ slightly each time the plate is read This behavior is expected Figure 5 9 Demonstration plate results Color Map layer When using the MESO SECTOR demonstration plate sectors 3 and 4 Figure 3 11 should display ...

Page 43: ...43 6 UsingtheSECTORS600 ...

Page 44: ...es from the right side and eject plates into the left side If the stacker cover plate is present on the output stack remember to select the Return Plates to Input Stack checkbox so that the SECTOR S 600 will not attempt to place the plate into the covered stack at the end of the read Prepare the MULTI ARRAY plate using the required reagents per the assay protocol Place the plate into the single pl...

Page 45: ... tube on the input right stacker interface plate 7 Place an empty stack tube of the same size on the output left stacker interface plate Figure 6 1 Loading MULTI ARRAY plates into stack tube If the stack of plates to be run is too tall for the above procedure you can insert the additional plates through the top of the stack tube up to the stack tube capacity during the run Be careful to not to add...

Page 46: ...f the output stack The plates should be removed manually by sliding them up and out the top of the stack tube The last few plates can be accessed by pushing the last plate up from the bottom of the stack tube 6 3 Robotics Integration The SECTOR S 600 instrument can be integrated with a robotics system for loading and unloading plates using the Remote Instrument Mode which allows the instrument to ...

Page 47: ...47 7 Maintenance ...

Page 48: ...eventive Maintenance Scheduled maintenance should include the cleaning and lubrication of all appropriate internal components Only MSD Service Engineers should perform this maintenance usually once every 6 months Contact MSD Customer Service to ask about our service contracts 7 2 Instrument Cleaning Reasonable care should be taken to prevent unnecessary fluid spills onto and into the SECTOR S 600 ...

Page 49: ...for 15 minutes Rinse the latches thoroughly with running water tip and or shake gently to remove excess liquid and allow to fully air dry If there is any residue remaining soak the latches in a 70 alcohol solution isopropanol or ethanol in water for 15 minutes shake out the excess and allow to air dry completely If plate loading problems persist contact Scientific Support 7 3 Instrument Decontamin...

Page 50: ...50 8 Appendix ...

Page 51: ...e Run button Windows security settings networking parameters or the instrument name has changed Click OK to continue Determine whether your IT department has made any changes to the instrument computer recently Incompatible anti virus software has been installed on the instrument computer Disable the antivirus program temporarily to see if that is the issue There are several methods 1 Exclude the ...

Page 52: ...restarting the software will resolve it If the service has been interrupted it must be restarted This can be accomplished by restarting Windows or the service can be manually restarted as follows 1 Right click My Computer on the desktop and select Manage In the Computer Management window under Services and Applications click Services 2 In the alphabetical list of services right click SQL Server ME...

Page 53: ...d and secure Close DISCOVERY WORKBENCH and restart the computer If restarting does not correct the problem then switch the location of the instrument to computer USB cable by plugging the USB cable into a different USB port on the instrument computer 1 Turn off the SECTOR S 600 2 Wait 30 seconds 3 Turn on the SECTOR S 600 4 Restart the computer 5 Wait five minutes before starting the MSD DISCOVERY...

Page 54: ...000 counts 25 100 counts Dynamic Range 10 6 Electronic Noise Standard Deviation Dark Noise one spot plates 16 counts 8 2 3 Environmental Specifications Operational Temperature Range 20 26 C 68 78 F Operational Humidity Range 10 80 non condensing Storage Temperature Range 5 40 C 41 104 F Storage Humidity Range 5 85 non condensing Ambient Light 2000 Lux 8 2 4 Power Requirements Voltage 100 240 V 50 ...

Page 55: ...e 128 Contact MSD Scientific Support for information on proper label font size and location of custom bar code on plate Recognition of additional codes is possible but has not been tested 8 2 8 Plate Specifications MSD MULTI ARRAY and MULTI SPOT plates are designed to comply with the mechanical dimensions of the proposed standards of the Society of Biomolecular Screening Contact MSD Scientific Sup...

Page 56: ...56 9 TechnicalSupport ...

Page 57: ...ic tool as an administrator A copy of the tool is located in Win7 32 bit C Program Files MSD DISCOVERY WORKBENCH bin The instructions for running this tool are as follows 1 Hold down the Shift key and right click on the SysInfo exe file 2 Select Run as administrator and enter administrator credentials if requested 3 Wait about 5 minutes to allow the tool to complete creating the file 4 Browse to t...

Page 58: ...gn R PLEX design S PLEX design U PLEX design V PLEX design It s All About U and SPOT THE DIFFERENCE are trademarks and or service marks of Meso Scale Diagnostics LLC All other trademarks or service marks are the property of their respective owners 2013 2017 Meso Scale Diagnostics LLC All rights reserved Customer Service Phone 1 240 314 2795 Fax 1 301 990 2776 Email CustomerService mesoscale com Ho...