MshOt MF53-N, User Manual

Page 1: ...Guangzhou Micro shot Technology Co Ltd MF53 N Inverted fluorescence microscope User manual Website www m shot com Tel 0086 20 38262481 Email sales mshot com ...

Page 2: ...Inverted fluorescence microscope MF53 N ...

Page 3: ...e or power cord may cause damage or fire to this unit Any extended power cord used must be grounded PE 5 Protect this unit from high temperatures dampness and foreign objects To prevent short circuit or any other fault do not expose this unit to any high temperatures or dampness environment for a prolonged period of time A suitable operating environment is designated at a temperature of 5 C 35 C a...

Page 4: ......

Page 5: ... touch the glass surface of the bulb directly with bare hands When mounting the bulb wear gloves or wrap it with cotton material Wipe off any dirt on the surface of the bulb with a clean cotton fabric dipped in alcohol If the dirt is not thoroughly removed it would etch the surface of the bulb weakening its brightness and shortening its life Mount the bulb with care to avoid slipping off or injuri...

Page 6: ......

Page 7: ...Main observation steps 3 2 1 Bright field observation 3 2 2 Phase contrat observation 5 2 3 Fluorescence observation 6 3 Installation and debugging of microscope 8 4 Maintanence 12 5 Specification 14 6 Troubleshooting 错 误 未定义书签 ...

Page 8: ......

Page 9: ...1 1 Components LED light source bright field Phase contrast plate Condenser Stage Eyepiece Splitter lever Stage control wheel Focusing wheel Coarse and fine focusing system ...

Page 10: ...ht control box bright field Light barrier Nosepiece Broad spectrum LED lamp house Fluorescence module Camera port Microscope body Coarse and fine focusing wheel Broad spectrum LED power control box fluorescence ...

Page 11: ...pply to adjust the light source from 0 off to 100 brightest and adjust the light source to the appropriate brightness Push the splitter lever forward and switch to the eyepiece observation state The light path can be switched between eyepiece observation and eyepiece camera using the splitter lever Put the sample on stage Turn the objective nosepiece to turn the required multiple objective into th...

Page 12: ...t the light passing through is enough to ensure the best observation effect Remove the condenser barrier plate so that the light can reach the sample through the condenser Turn the dial on the right side of the fluorescent module to turn the neutral into the light path Adjust the focusing hand wheel to focus the sample When the right hand wheel of coarse focus and fine focus is rotated clockwise t...

Page 13: ...o that the phase difference ring of the corresponding multiple of the objective lens is in the optical path Adjust the adjusting rod of aperture diaphragm to the corresponding position Note the multiple of the phase contrast ring should correspond to the multiple of the objective lens If the 10x objective lens is used the 10x hole should be selected for the phase contrast insert plate Remove the c...

Page 14: ...t intensity knob to the appropriate brightness The left light intensity adjustment knob is the UV band brightness adjustment knob and the right light intensity adjustment knob is the B G band brightness adjustment knob At this time the bright field light source should be turned off Turn the objective nosepiece to turn the required multiple objective into the light path Note do not use phase contra...

Page 15: ...n use the fine focusing handwheel to focus precisely Adjust the tension of the coarse focusing hand wheel according to the user s needs Turn the tension adjusting ring of the coarse focusing hand wheel in the direction of the arrow to increase the tension On the contrary reduce the tension If the objective slides down due to its own gravity or defocuses rapidly after focusing with the fine focusin...

Page 16: ...cusing the right lens tube clearly observe the left lens tube again Adjust the diopter adjustment ring on the left eyeglass tube until the image is clear in the field of view 3 Installation and debugging of microscope Screw the objective on the objective nosepiece in order of multiple size Place four square gaskets on the stage bracket The wider gasket is placed on the wide bracket and the narrowe...

Page 17: ...o fix the stage Place the drip plate over the stage Open the dust cover of the eyepiece tube and clip the two eyepieces into the tube respectively Connect the bright field power cable on the microscope bracket to the bright field light source control box and connect the adapter on the other end to power on the microscope ...

Page 18: ...the lens properly observe the imaging in the centering telescope with one eye until two rings appear in the field of vision and then tighten the screw to fix the lens Use a hexagon key to adjust the left and right screws of the left and right 10x holes so that the bright ring and dark ring in the field of view of the centering telescope become concentric circles a b After adjustment take out the c...

Page 19: ... fix the light box Note when installing the light box hold the light box firmly and tighten the screws to ensure that the light box is fixed to prevent the light box from falling Pull out the plug of the power socket behind the fluorescent power control box and connect the wide spectrum LED light source lamp box with the power control box Pull out the plug of the power socket behind the fluorescen...

Page 20: ...ng Loosen the side fixing screw remove the dust cover at the camera interface clip the C type CCD adapter into the camera connection above the three lens barrel and tighten the side fixing screw Screw the camera digital CCD into the thread above the adapter and make sure the camera digital CCD is tight 4 Maintenance Cleaning of instruments The surface of exposed lens shall not be touched by hand T...

Page 21: ...instrument Indoor temperature 0 C 40 C Maximum relative humidity 85 If the microscope is used in high humidity area it is recommended to install dehumidification equipment to prevent the optical elements of the instrument from mildew and fogging When using and handling be careful to avoid strong vibration and collision When moving the instrument lift the instrument with both hands Do not drag on t...

Page 22: ...ence objective Plan Fluor 10X 0 3 WD 7 1mm Long working distance semi apochromats fluorescence objective Plan Fluor 20X 0 45 WD 5 9mm Long working distance semi apochromats fluorescence objective Plan Fluor 40X 0 65 WD 1 6mm Focus system Coaxial coarse and fine with limit and locking devices low coaxial focus adjusting handle Minimum adjustment gradations 1 µm Nosepiece Quintuple revolving nosepie...

Page 23: ... position turn the objective to make it enter the light path correctly wipe it clean with the wiping paper dipped in alcohol Dark on one side of the image The converter is not in position Turn to make it in place The specimen is floating Reliable reinforcement Image moving during focusing Specimen floating on the surface of the loading platform It should be placed firmly The converter is not in po...

Page 24: ...r poor contact Check the wire connection The light bulb is broken Replace The voltage is too low Increase the voltage Flashing or unstable light bulb Voltage problem Check voltage stability The bulb is not properly inserted into the socket Check and firmly plug it in ...

Page 25: ...17 ...