5
❾
On the basis of bright field observation, rotate the
objective nosepiece
④
to turn the 10x phase contrast
objective into the optical path.
The shell of phase contrast objective has the word "pH"
❿
Push the phase insert plate
⑤
into the slot of the phase
insert plate (refer to the installation of the phase insert plate
in the installation procedure of the microscope), so that the
phase difference ring of the corresponding multiple of the
objective lens is in the optical path. Adjust the adjusting rod
of aperture diaphragm to the corresponding position.
Note: the multiple of the phase contrast ring should correspond to the multiple of the objective lens. If the
10x objective lens is used, the 10x hole should be selected for the phase contrast insert plate
⓫
Remove the condenser barrier plate
⑥
so that the light
can reach the sample through the condenser.
⓬
Adjust the focusing hand wheel
⑧
to focus the sample.
Phase contrast observation
④
⑥
⑧
Summary of Contents for MF53-N
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