ML41 biological microscope user manual
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Picture 18 appears, and then adjust according to this proportion.
Use of scale: adjust the condenser numerical aperture scale to
80% of the numerical aperture
②
marked on the objective. For
example, when using a 40x objective (the numerical aperture is 0.65),
adjust the marking line of the aperture at 0.52 (0.65*0.8).
3.11 use of oil immersion objectives
(1) Focus on the specimen under 4x objective.
(2) Drip one drop of oil on observation part. (Picture 20)
(3) Turn the nose-piece and shift the oil immersion objective in, and
using the fine focal knob to focus on the specimen.
☆
Make sure no bubbles in the oil because they can affect the
imaging.
a.When checking the bubbles, remove the eyepiece and open the field
diaphragm and aperture diaphragm thoroughly. Look through the
eyepiece barrel and observe the outline of objective’s aperture (it
should be round and bright).
b.When eliminating the bubbles, slightly turn the nose piece and wave
the oil immersion objective back and forth for several times.
(4) After finished using the oil immersion objective, carefully wipe
the front lens with clean gauze and or lens wipes dipped in Ethanol
ethyl ether mixture (mixed with 3:7 ) or xylene.
☆
Do not shift other objectives in before the oil immersion
objective was cleaned, or the dry objectives may stained by oil.
☆
Too much xylene can dissolve the adhesive on the objective
lens.
3.12 use of color filter
Color filter can moderate the background light and improve the
contrast of the image. (Picture 21)
The filters have four colors namely yellow, green, blue and white.
☆
The filters shall be installed with blurred side downward.
3.13 use of the reticle
(1) Spin the reticle
①
off the pressing ring.
(2) Put the reticle in the pressing ring
①
, with scale
②
upward.
(3) Install the pressing ring
①
equipped with reticle into the
eyepiece. (Picture 22)
☆
The size of the reticle
￠
24
×
1.2mm. Attention: The scaled side
shall be upward.
oil
Picture 20
Picture 21
Picture 22
