MultiChannel MPA32I, Manual

Page 1: ...32 Channel Miniature Preamplifier Manual ...

Page 2: ...he publisher and the author be liable for any loss of profit or any other commercial damage caused or alleged to have been caused directly or indirectly by this document 2018 Multi Channel Systems MCS GmbH All rights reserved Printed 16 04 2018 Multi Channel Systems MCS GmbH Aspenhaustraße 21 72770 Reutlingen Germany Phone 49 71 21 909 25 0 Fax 49 71 21 909 25 11 sales multichannelsystems com www ...

Page 3: ...PA 4 3 1 General Setup Recommendations 4 3 2 Testing the Noise Level of the Setup 5 4 Setting up MC_Rack 6 5 Connecting FlexMEAs 8 5 1 EcoFlexMEAs 10 5 1 1 Connecting 32 Channel NeuroNexus Probes 11 5 1 2 Adapter NN 32 Pin Layout 12 5 2 Connecting 54 Channel NeuroNexus Probes 13 6 Service and Maintenance 15 7 Pin Layout 16 7 1 Power Supply 16 7 2 Test Model Probe 17 8 Contact Information 18 9 Tech...

Page 4: ...e these no later than on conclusion of the contract Multi Channel Systems MCS GmbH makes no Guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software It is up to the user to use good laboratory practice to establish the validity of his findings Guarantee and liability claims in the event of injury or material damage are excluded when they are the...

Page 5: ... device may only be used for its intended purpose be used when in a perfect condition Improper use could lead to serious even fatal injuries to the user or third parties and damage to the device itself or other material damage Warning The device and the software are not intended for medical uses and must not be used on humans Malfunctions which could impair safety should be rectified immediately A...

Page 6: ...as current The high input impedance ensures stable long term recordings Ideally the input impedance would be infinite As low voltages are generally recorded a high current would flow if the input impedance were low As a result the amplifier would not be able to deliver the current and the voltage would break down The miniature preamplifier has a high input impedance to avoid this problem Adapters ...

Page 7: ...preamplifier to the common ground of the setup The GND input is internally connected to the metal case 3 Connect the reference electrode to the reference input of the miniature preamplifier Generally a reference electrode is inserted into non active tissue of the experimental model The reference electrode should be identical to the recording electrode so that both electrodes see the same backgroun...

Page 8: ...ting the Noise Level of the Setup A terminal plug that connects all inputs to ground and a test model probe that mimics the electrode impedance are available for testing the noise level of the setup Note Please note that the test model probe has to be ordered separately All instruments were thoroughly tested at the factory site before delivery The suggested tests are mainly intended for optimizing...

Page 9: ...lifier with a gain of 10 and a following filter amplifier with a gain of 100 the total gain is 1000 Select an input voltage range of 819 2 to 818 8 mV and a sampling rate of 10000 Hz On the Edit menu click Add Data Display to add a raw data display to your virtual rack In the virtual rack tree view pane select the Display 1 and click the Layout tabbed page Set up a channel map with channels 1 to 6...

Page 10: ...enerally expect a slightly lower noise level Noise level test with MPA8I and FA64I 100 0 5 5000 filter amplifier The noise level is 60 µV peak to peak with the provided test model probe channels 1 32 and 20 µV peak to peak with grounded inputs channels 33 64 The top window shows raw data the bottom window shows the extracted peak to peak amplitudes of channels 1 and 33 The miniature preamplifier w...

Page 11: ...e sides of the white slot cover with your fingers and carefully pull open the slot cover only a little bit about 1 or 2 mm Do not demount the cover as the plastic supports might break easily Insert the FlexMEA36 into the slot of the drawer with the contact pads upside down Insert the plastic spacer into the slot of the drawer Important Please use a thin plastic spacer between the FlexMEA36 and the...

Page 12: ...s and four ground electrodes The flexible base is perforated for a better contact with the surrounding tissue The electrodes have a diameter of about 100 µm with an interelectrode distance from 625 to 750 µm Contact pads and conducting material is pure gold Using FlexMEAs FlexMEAs are usually connected to a headstage preamplifier that is connected to a filter amplifier or programmable gain amplifi...

Page 13: ...FlexMEA24 has 24 gold electrodes plus two internal reference electrodes and two ground electrodes The flexible base is perforated for a better contact with the surrounding tissue The gold electrodes have a diameter of about 80 µm with an interelectrode distance of 300 µm and an impedance of approximately 50 kOhm Contact pads and conducting material is pure gold Using EcoFlexMEAs EcoFlexMEAs are us...

Page 14: ...ortant REF has to be connected for obtaining a proper signal Insert the pin array of the NeuroNexus probe into the black connector of the ADPT NN 32 Connect the MPA32I to the adapter outputs Make sure that the orientation of the MPA32I is correct see illustration ...

Page 15: ...ows the assignment to the electrode numbers as given by NeuroNexus Technologies Pin Layout ADPT NN 32 MPA32I Software ADPT NN 32 Pin No Channel Electrode 1 GND 2 REF REF 3 CH 1 EL 17 4 CH 2 EL 21 5 CH 3 EL 18 6 CH 4 EL 23 7 CH 5 EL 19 8 CH 6 EL 25 9 CH 7 EL 20 10 CH 8 EL 27 11 CH 9 EL 29 12 CH 10 EL 31 13 CH 11 EL 32 14 CH 12 EL 30 15 CH 13 EL 28 16 CH 14 EL 26 17 CH 15 EL 24 18 CH 16 EL 22 19 CH ...

Page 16: ...2 34 CH 32 EL 16 35 REF 36 GND 5 2 Connecting 54 Channel NeuroNexus Probes Important REF has to be connected for obtaining a proper signal Connect the two MPA32I to the ADPT NN 54 adapter outputs Make sure that the orientation of the amplifiers is correct see illustration ...

Page 17: ...MEAs 14 ADPT NN 54 Pin Layout Numbers in italics are the MPA32I pin numbers The electrode numbers are aligned to the input pins of the MPA32I The next figure shows the numbering of the polytrode electrodes ...

Page 18: ... and treat them in an ultrasonic bath for 10 to 20 s Air dry the miniature preamplifier for about 5 min before use You can also clean the connectors with distilled water in an ultra sonic bath Dry the connectors with compressed air immediately after sonication and let the amplifier air dry for at least 6 h before use Sterilization Warning Do not autoclave or sterilize miniature preamplifiers by hi...

Page 19: ...ction may damage the unit Input Connector This illustration shows the pin layout viewed from the front with the case screws upside down The two reference inputs are used for connecting a reference electrode Both inputs are equal so it does not matter which of the two inputs is used If both are used the mean of both reference signals is used as reference Output Connector The signal ground is connec...

Page 20: ...nal distribution The input signal is distributed across all recording channels REF I N The standard situation in an experiment should be that the reference electrode has properties identical or close to the recording electrodes You can remove the jumper to connect the reference output of the test model probe to the same resistors as the recording channels to mimic the experimental situation or you...

Page 21: ... an excellent opportunity for you to exchange your experience or thoughts with other users worldwide Mailing list If you have subscribed to the ME System mailing list you will be automatically informed about new software releases upcoming events and other news on the product line You can subscribe to the list on the contact form of the MCS web site www multichannelsystems com ...

Page 22: ...nnector type Dual row precision sockets 50 mil 1 27 mm grid pattern for 0 35 to 0 45 mm round pins Supply voltage 3 V to 8 V DC Supply current 14 mA typically 9 mA Number of input channels 32 Input voltage 500 mV with respect to a supply voltage of 5 V Input impedance 1012 parallel to 10 pF Input noise 1 5 µVRMS 1 Hz to 5 kHz inputs short circuited Noise density en 15 nV Hz Number of output channe...