FlexMEAs
4
3
Setting Up and Connecting the MPA
3.1
General Setup Recommendations
In the following, you find general recommendations for the installation.
Important: It is important that the complete setup refers to a single common ground. The
reference input has always to be connected. It is recommended to use a reference electrode.
However, if you are not using a reference electrode, connect the reference input to ground
(GND). Otherwise, noise picked up by the reference input will be subtracted from the recording
signals. This will either lead to signal loss or to a very high noise level.
1.
Ground the animal with a
ground electrode
of
large surface area
, for example, a
liquid gel
adhesive electrode
,
that is connected to the
ground
of the setup, for example, a
large metal
table
or a
Faraday cage
, to avoid pickup of noise from the environment. The ground electrode
is best positioned in an electrically inactive region (not near muscle, nor heart), for example, at the
belly
.
2.
Connect the
GND input
or the
metal case
of the miniature preamplifier to the
common
ground
of the
setup
. (The GND input is internally connected to the metal case.)
3.
Connect the
reference electrode
to
the reference input
of the miniature preamplifier.
Generally, a reference electrode is inserted into non-active tissue of the experimental model.
The reference electrode should be identical to the recording electrode so that both electrodes see
the same background noise. This is necessary because despite the grounding, the animal’s body
often has not exactly a potential of zero, due to the electrode impedance, for example. The
background noise is then subtracted from the recording signal, increasing the signal to noise ratio.
Please note that this may not work if the complete setup is not properly grounded.
4.
(Optional) If two reference electrodes are used, connect the second electrode to the free
reference input. Otherwise, leave it free. It is generally not necessary to use a second reference
electrode. It can be used to enlarge the surface area of the reference, though.
5.
Connect the
recording electrodes
to the
recording channels
of the miniature preamplifier.
You can either use single electrodes or multitrodes with the miniature preamplifier. MCS provides
adapters for standard probes.