ADAM-MC (Automated Cell Counter) ©2008 NanoEntek, Inc.
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Introduction
Overview
Until now,cell counting and viability measurement for many types
of cells have been performed manually using hemocytometer
with TrypanBlue exclusion method, which is to distinguish
viable cells from non-viable cells. One drawback of this
method, however, is the propensity for the staining of
artifacts; another drawback is that the naked eye can only
differentiate between cells in a limited concentration range in
the hemocytometerchamber. This combined with the potential
problem of cell aggregation and limited sample volume leads
to the common variation of counts normally associated with this
method.
To address these problems, NanoEntek has developed the
ADAM-MC, which is based on a fluorescent microscopy
technique for counting cells. The ADAM-MC utilizes sensitive
fluorescence dye staining, LED optics and CCD detection
technologies to make the cell analysis more accurate and reliable.
To count cells using ADAM-MC, the cells are mixed with a
Propidium Iodide (PI) stain and directly pipettedon to a disposable
plastic chip. The chip is then loaded onto a precision stage.
An ADAM-MC system is automatically focused onto the chip
and cells that have been stained are recorded by a sensitive
CCD camera. The image results are automatically processed
generating the cell count which is displayed on the front of the
instrument.
Simple. Fast. Accurate. Reliable.
