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3. Introduction
Instrument description and main features
Plug & Play systems
• 2 imaging modalities:
- Physical structural imaging (based on refractive index, RI)
- Chemical fluorescence imaging
• 3 Fluorescence Channels: 2 configurations available:
- DAPI (Excitation 392 nm / Emission 432 nm), FitC (Excitation 474 nm /
Emission 515 nm), TritC (Excitation 554 nm / Emission 595 nm)
- FitC (Excitation 474 nm / Emission 515 nm), TritC (Excitation 554 nm /
Emission 595 nm), Cy5 (Excitation 635 nm / Emission 730nm)
• 4 Fluorescence Channels:
- DAPI (Excitation 392 nm / Emission 432 nm), FitC (Excitation 474 nm /
Emission 515 nm), TritC (Excitation 554 nm / Emission 595 nm), Cy5
( Excitation 635 nm / Emission 730nm)
Note: TritC and Cy5 excitation filters are exchangeable on channel 3
Please refer to the following link for details on the Filter configuration:
https://www.semrock.com/SetDetails.aspx?id=3297
Likewise, refer to the following link for further data on the LED source:
http://www.coolled.com/product-detail/led-wavelengths/
• Operation modes:
- 2D epifluorescence snap-shot (every second) & time-lapse (up to 96 hours)
- 3D RI snap shot & time-lapse 3D RI & 2D epifluorescence snap-shot (every
3 seconds) & time-lapse (up to 96 hours)
• Co-localisation of Fluorescence (3 channels) and Digital Stains (7 channels at
single acquisition)
• Time-lapse possibility up to 96 hours
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