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Step 5.:

 Switch the Halogen lamp OFF. 

Step 6.:

 Switch OFF the Microscope body with the switch on the optical table. 

Step 7.:

 Switch OFF the stage controller and wait 10 seconds. 

Step 8.:

 Switch OFF the confocal controller besides the lasers by pressing the button on the 

front panel. 

Step 9.: 

 

 

Power OFF the 488nm (green) and 647nm (far red) lasers. The 647nm (far red) laser can 
be powered OFF with the key on the remote control. The 488nm Argon Ion laser switched 
to standby mode by switching the "discharge" flip switch to OFF on the controller on the 
laser board unit., 

Step 10.:

 Shut down the laser board with the key on its front panel. 

Step 11.:

 Switch off the computer and check if everything is off before leaving. 

 

Summary of Contents for A1R

Page 1: ...Fold the lens cleaning paper three times to form a strip 2 Hold it on the two ends on both sides 3 gently wipe with left right motion Never press with your finger directly 4 Repeat previous step until you cannot see oil stain and repeat once after that one final time 7 When finished ALWAYS turn to the 4x objective and lower the stage to a safe distance Sample Holder 1 When using the universal samp...

Page 2: ...ng a booking please send an e mail to the previous subsequent user and the institute mailing list 3 IN VIVO experiments can be only started in the AFTERNOON as the drift caused by cooling and heat within the chamber will affect following users as well System Start up Step 1 Switch on the control computer Step 2 Power up the laser board by turning the key on the controller front panel of the laser ...

Page 3: ...er that is situated to the left of the microscope The switch is on the left side roughly in the middle Step 5 Switch on the stage controller on the table and wait 10 seconds Waiting is important as Nikon microscopes are highly customizable and the microscope body checks which peripheries are currently connected Step 6 Switch on the Microscope body with the RED switch on the optical table Step 7 Op...

Page 4: ... the 60x oil immersion lens has been used NEVER forget to clean with lens cleaning paper 1 Fold the lens cleaning paper three times to form a strip 2 Hold it on the two ends on both sides 3 Gently wipe with left right motion Never press with your finger directly 4 Repeat previous step until you cannot see oil stain and repeat once after that one final time Step 3 Stop NIS elements If you proceed w...

Page 5: ...e button on the front panel Step 9 Power OFF the 488nm green and 647nm far red lasers The 647nm far red laser can be powered OFF with the key on the remote control The 488nm Argon Ion laser switched to standby mode by switching the discharge flip switch to OFF on the controller on the laser board unit Step 10 Shut down the laser board with the key on its front panel Step 11 Switch off the computer...

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