Nikon DS-2MBW, Instruction Manual

Page 1: ...DS Camera Control Unit DS L1 DS Camera Head DS 5M DS 2Mv DS 2MBW DS Cooled Camera Head DS 5Mc DS 2MBWc Instructions Ver 3 0 M306E 05 04 CF 7 1 2 ...

Page 2: ......

Page 3: ...ailure to observe the instructions may result in injury or property damage To ensure correct use read this manual carefully before using your products Do not discard this manual Always keep it at hand for easy reference Safety instructions in this manual are indicated by the symbols shown below To ensure safety please follow the instructions indicated by these symbols Symbol Meaning Disregarding i...

Page 4: ...ment falls under the category of electric shock protection class I always make sure it is grounded to a protective ground terminal If you misplace or damage the power cord contact your nearest Nikon representative When using the product in 100 120 V AC regions Use an UL listed detachable power cord set 3 conductor grounding Type SVT No 18 AWG 3m long maximum rated at 125V AC minimum When using the...

Page 5: ...equipment or when connecting disconnecting cables To prevent damage or malfunction always turn off the DS L1 s POWER switch when setting up the equipment or when connecting or disconnecting cables 3 Connect only the devices specified to the connectors To prevent damage to the equipment connect only the devices specified to the connectors on the DS L1 the DS camera head or the DS Remote Controller ...

Page 6: ...onfiguration and Method of Displaying a Menu 26 1 3 1 Menu Configuration 26 1 3 2 Method of Displaying a Menu 27 1 4 Initial Settings 28 1 4 1 Date and Power save Settings Additional Settings 28 1 4 2 Image File Settings File Settings 30 1 4 3 Startup Condition Settings Power On Settings 36 1 4 4 Adjusting Monitor Output for an External Monitor 37 1 4 5 Settings for Direct Print 38 1 4 6 Settings ...

Page 7: ...ust the Subject Position 78 3 5 2 Using a Scale to Measure the Subject Size 80 3 5 3 Using XY Scales to Measure the Dimensions of the Subject 81 3 5 4 Creating a Translucent Image and Comparing It to a Live Image 82 3 5 5 Measuring vertical and horizontal distances 83 4 Using the DS L1 via a Network 84 4 1 Typical Method of Use 84 4 2 Network Settings 86 4 2 1 Items Needing Confirmation 86 4 2 2 S...

Page 8: ... 4 4 Using the CCD Image Drive 128 5 4 5 Removing the DS L1 from the PC 130 6 Using the Microscope Control 131 6 1 Overview 131 6 2 Menu Overview 131 6 2 1 Microscope Control Menu 1 137 6 2 2 Microscope Control Menu 2 145 6 2 3 Microscope Status Display Microscopy Setup Menu MIC Info Menu 148 6 2 4 Ergo Controller button information display menu 151 6 2 5 Connecting a Motorized U EPI 152 6 2 6 Con...

Page 9: ...CONTENTS vii 3 DS Remote Controller DS RC 169 4 DS AC adapter 170 5 Overall Specifications 170 ...

Page 10: ......

Page 11: ...white 2 110 000 pixels Yes Black For detailed information on the features of each DS camera head model refer to Chapter IX Technical Specifications Indication of DS camera head model name In this manual the product names DS Camera Head DS 5M DS Camera Head DS 2Mv DS Camera Head DS 2MBW DS Cooled Camera Head DS 5Mc and DS Cooled Camera Head DS 2MBWc are abbreviated DS 5M DS 2Mv DS 2MBW DS 5MC and D...

Page 12: ...issing contact your nearest Nikon representative immediately 1 DS Camera Control Unit DS L1 5 DS camera head C mount cap Camera cable 3 m E229586 2 Instruction manual 6 DS Remote Controller DS RC Remote cable E120411 3 DS AC adapter 7 Direct Print License optional 4 Power cord 8 C mount adapter optional 9 Microscope Control License optional ...

Page 13: ... until the DS L1 has started up This forcibly sets the DS L1 to internal monitor output and external XGA output modes when it starts up After the DS L1 has started up set to XGA in Power On Settings of the SETUP menu and save your settings NOTE If you are using an LCD display or LCD projector we recommend adjusting its clock phase and clock pitch 2 USB mouse You ll need a USB mouse to access funct...

Page 14: ...ve 1 to 4 GB 7 C mount lens A C mount lens is required to photograph subjects other than photomicrographs Any lens that satisfies the following conditions may be used Protrudes no more than 10 mm from the mount surface into the camera The image circle is 2 3 for the DS 5M and DS 5Mc and 1 1 8 for the DS 2Mv DS 2MBW and DS 2MBWc Use a lens for 2 3 or larger e g 1 When using diaphragm settings brigh...

Page 15: ...tions DS L1 operations cannot always be guaranteed since they are affected by OS or browser versions and by various software settings 11 Nikon 80i 90i Microscope LV150A Industrial Microscope If you have a Nikon 80i 90i microscopes equipped with a digital imaging head you can purchase a license to control the microscope or display its status using the menus on DS L1 To connect your Nikon 80i 90i mi...

Page 16: ...p a menu After a menu is displayed the MENU button can be used to confirm a selected menu command just like the ENTER button POWER switch This is a push switch Press it to turn on the power Press again to release it and turn off the power When the power is on the LED indicator lights and remains on ZOOM Electronic zoom button FREEZE Freeze pause button The freeze LED lights when the display is fro...

Page 17: ...USB keyboard USB printer CP900D or Nikon microscope Network Tx indicator Lights when sending data to a LAN Network Rx indicator Lights when receiving data from a LAN LAN port Connect the DS L1 to a LAN with a LAN cable to exchange data with a PC or server through a LAN 12 V DC output This output is currently unused 12 V DC input Use a dedicated AC adapter Stand arm Extract the arm to use it CF car...

Page 18: ...ods in Chapter IV for details on the interface specifications External interface input Connect the DS Remote Controller DS RC here and you can perform freeze and capture operations from the DS RC A foot switch etc may also be used providing it meets the interface specifications Refer to 3 Connection Methods in Chapter IV for details on the interface specifications Camera connector Connect the DS c...

Page 19: ...ctly to a printer can be assigned Above functions can be assigned to the button in Additional Settings of the SETUP menu FREEZE button Freezes the current image as a still picture To release the frozen image press the button again C mount cap Protects the C mount from dust C mount When taking microphotographs mount the C mount adapter here before connecting to the microscope When taking other phot...

Page 20: ...amera head CF card DS L1 DS AC adapter USB cable USB equipment mouse keyboard printer and Nikon misroscope LAN cable DS RC IV Connecting and Installing the Equipment 1 Connection Diagrams Schematic diagram PC or a PictBridge supporting printer DS camera cable LAN RGB cable ...

Page 21: ... Diagrams 11 Connectors DS L1 Left side DS L1 Rear RGB cable External monitor Remote cable DS RC Remote cable DS camera head USB cable USB equipment LAN cable PC or a PictBridge supporting printer DS AC adapter USB mouse USB keyboard Printer Microscope LAN ...

Page 22: ...vent heat from collecting near the equipment When attaching a commercially available arm to the DS L1 Any commercially available VESA standard compliant 75 mm arm UL1678 suited stand and mount can be attached to the DS L1 When doing so make sure the following specifications are met DS L1 weight 1 3 kg Fitting screw hole M4 7 mm deep Tightening torque 80 120 N cm Leave a clearance of about 100 mm a...

Page 23: ... far as it will go 4 Insert the C mount adapter into the vertical tube of the microscope Use the clamping screw on the vertical tube to secure the C mount adapter in place You can also attach a relay lens We recommend the x0 7 relay lens for the DS 5M and DS 5Mc We recommend the x0 55 relay lens for the DS 2Mv DS 2MBW and DS 2MBWc When you have mounted the DS camera head on a microscope be sure to...

Page 24: ... USB D connector on the DS L1 When connecting to a PictBridge supporting printer connect the PictBridge supporting printer and the USB D connector on DS L1 with a USB cable AB type 5 Connecting the DS Remote Controller DS RC Be sure to turn off the power for the DS L1 before making any connections Connect one end of the remote cable supplied with the unit to the EXTERNAL IN connector on the DS L1 ...

Page 25: ...e obtained when capturing an image If you are preparing your own external device use a device that satisfies the specifications shown below Connector 3 5 mm diameter stereo pin jack EXTERNAL IN connector Connector 2 5 mm diameter stereo pin jack EXTERNAL OUT connector EXTERNAL IN Functions Pin 1 FREEZE Pin 2 CAPTURE You will not be able to capture desired images at precise split second moments Use...

Page 26: ...gging a stroboscope or other external device into the EXTERNAL OUT connector of the DS L1 make sure the power for the external device is turned off If the power for the external device remains on your eyes may be damaged by unintended light emission 8 Connecting to a Power Supply To prevent electric shock turn off the power for the DS L1 before making any connections Complete all other connections...

Page 27: ...he illumination optical path of the microscope to compensate for changes in color balance caused by the length of the exposure time When using a phase contrast microscope or an interference microscope two luminous fluxes or multiple luminous fluxes you can enhance contrast using a green interference filter GIF or a monochrome interference filter IF We recommend simultaneous use of an NCB filter an...

Page 28: ...se down the aperture diaphragm Note that closing down the aperture may limit the performance of the objective Terms What is depth of focus Depth of focus refers to the range along the axis of the field of view over which the image is in apparent focus Although reducing the aperture diaphragm extends the depth of focus doing so will also reduce resolution Take this into consideration and adjust the...

Page 29: ...ing a dark specimen with a fluorescent microscope for example ambient indoor light may enter the optical path Accordingly interior lighting should be darkened for photomicrography Place caps on the binocular eyepiece to prevent light from entering through the eyepiece 8 Effects of Vibration Because photomicrography involves extremely high image resolution the slightest vibration can affect image q...

Page 30: ...n cooled DS 2MBW 2 megapixel CCD monochrome non cooled DS 2MBWc 2 megapixel CCD monochrome cooled For detailed information on these cameras refer to Chapter IX Technical Specifications The following terms are used in this manual to refer to different cameras 5M cameras DS 5M and DS 5Mc 2M cameras DS 2Mv DS 2MBW and DS 2MBWc Cooled cameras DS 5Mc and DS 2MBWc Non cooled cameras DS 5M DS 2Mv and DS ...

Page 31: ... Using Various Input Devices 1 1 1 Using the DS L1 Panel Switches MENU button menu enter button Pressing the MENU button brings up the CAM Easy menu While the menu is displayed use this button as the ENTER button to confirm your selection MENU button Cursor buttons ZOOM button FREEZE button CAPTURE button ...

Page 32: ...xposure method on the CAM menu Select an icon you want and use the right or top cursor button to select items in the forward direction or the left or bottom cursor button to select items in the reverse direction ZOOM button electronic zoom button Use this button to apply an electronic zoom The image zooms in or out at the magnification selected for E Zoom Step in Additional Settings of the SETUP m...

Page 33: ...lue while right clicking the icon reduces the value For a shutter speed Left clicking reduces the shutter speed to increase exposure right clicking increases the shutter speed to reduce exposure For a mode select icon such as one to choose an exposure method on the CAM menu Left clicking lets you select items in the forward direction right clicking lets you select items in the reverse direction So...

Page 34: ...yboard F1 CAM menu F2 VIEW menu F3 REC menu F4 TOOL menu F5 F6 MIC menu F7 F8 F9 E ZOOM F10 E ZOOM F11 FREEZE F12 CAPTURE Back Space Deletes one character during character entry SHIFT Backspace Deletes the entire text during character entry NOTE The DS L1 is factory set to a Japanese keyboard layout If you wish to use an English keyboard issue the Telnet command shown below to change keyboard sett...

Page 35: ...atted in FAT16 For detailed information on using the formatting function refer to 2 2 2 Reproducing Images from a CF Card VIEW Menu in Chapter VI NOTE CF cards formatted in other than NTFS may not be correctly formatted in FAT16 depending on the formats used In such cases reformat those CF cards in FAT12 FAT16 or FAT32 on your computer before use Insert the connecting side with the connecting slot...

Page 36: ...s MIC Microscope control menu SETUP Power ON Settings Network Settings File Settings Additional Settings Startup settings Network settings File settings Other environment settings Return to window to return to the upper layer Shortcut Buttons for frequently used functions Freeze Electronic zoom AE lock Print Save to FTP server Save to CF card MIC INFO Settings of the frequently used status display...

Page 37: ...s the button Using the mouse Right clicking with the mouse displays a menu The specific menu displayed depends on the mouse pointer position on the screen when you right click Top 1 3th of the screen CAM Easy menu Middle of the screen CAM Easy menu and shortcut menu Bottom 1 3th of the screen Shortcut menu To close the menu screen right click anywhere outside the menu area Using the keyboard Press...

Page 38: ... for saving a log file at the same time 3 Startup condition settings Set startup conditions for photography and display states To make these settings display a menu and select the SETUP tag to enter the SETUP menu The correct procedures are detailed below 1 4 1 Date and Power save Settings Additional Settings Display a menu and select the SETUP tag to enter the SETUP menu Select Additional from th...

Page 39: ...on rate of the electronic zoom at which rate the image is zoomed in or out Two or more choices can be selected Capture Function Save to Card Save to Server Print Select one of these functions to save to a CF card to save to the FTP server or to output to a printer you want assigned to the CAPTURE button on the DS L1 and the DS Remote Controller DS RC or to the CAPT button on the menu The CAPTURE b...

Page 40: ...g the date Example If the specified folder name in Card Dir is IMGBOX with the Auto check box selected and the date is 2005 03 28 then a folder named IM050328 will be created File Name Specify up to four leading characters for the name of the file in which you want to save an image A photographed image is saved under a file name comprising the specified file name and an automatically assigned seri...

Page 41: ...printing images directly Use this setting to ensure the image quality level for saved images You can also use this setting to avoid switching CCD modes when freezing images For 5M cameras choose s5M or s1 3M For 2M cameras choose s2M or s800 For setting guidelines refer to Source Setting Guidelines Photography mode on the next page If you set the source to the highest resolution s5M for 5M cameras...

Page 42: ... overlays with the TOOL menu keep in mind that it may be difficult to read text information if the file is too small or features high JPEG compression We recommend saving files that include text information in BMP format at 1280 960 resolution or higher Source Setting Guidelines Photography mode The source setting represents the CCD mode during image acquisition when you freeze save or print image...

Page 43: ...e monitor resolution The image is displayed at actual size 1280 960 SXGA or reduced 1024 768 XGA The frame rate is 7 5 fps This display mode is useful when good performance with moving subjects is required If the source is set to s5M the CCD mode will switch to match the shutter speed resulting in delays during image acquisition Progressive display modes eliminate color shifts even with moving sub...

Page 44: ...rmance with moving subjects is required This mode is four times more sensitive to light than other modes making it is particularly useful for dark subjects If the source is set to s2M the CCD mode switches to match the shutter speed resulting in delays during image acquisition Progressive display modes eliminate color shifts even with moving subjects 800 5 An image area of 800 560 pixels is enlarg...

Page 45: ...lets you observe bright subjects at high speed or dark subjects at high resolution without manually adjusting the display mode However note that the display mode will not adjust automatically when set to maximum resolution 5Mi for 5M cameras or 2Mfull for 2M cameras or C Scan Specifically the display mode is adjusted as follows 5M cameras 1 3Mp 1 3Mi Shutter speed 1 3 second 5Mi Shutter speed 1 7 ...

Page 46: ...he custom mode to be used at startup NOTE The contents of the custom mode can be set from the CAM menu Cam OSD Ind Bio Select the type of scene mode button to be displayed in the CAM Easy menu Cross Line Selects whether to display or hide crosshairs when the power is turned on Refer to 3 5 1 Using Crosshairs to Adjust the Subject Position in Chapter VI Display Custom No 1 7 Seven types of screen d...

Page 47: ...nitor Output for an External Monitor If the DS L1 is connected to an external monitor you must adjust the monitor output on the Power On Settings screen Monitor output is initially set to 1 024 768 resolution 60 Hz XGA If observation with a higher definition display is required when photographing an image we recommend using 1 280 1 024 resolution 60 Hz SXGA output NOTE If you select SXGA output im...

Page 48: ...me to warm up sufficiently in accordance with the instruction manual 3 Display a menu and select the SETUP tag on it to enter the SETUP menu Select Additional from the SETUP menu to go on to the Additional Settings screen 4 Press the LCD Chart at the lower right corner of the screen A 1 dot pitched monochrome mesh chart will be displayed 5 Adjust the clock phase and clock pitch on the display side...

Page 49: ...pporting printer Or as another method connect a PictBridge supporting printer to the USB D connector on DS L1 first then enter a check mark in the PictBridge check box and turn the power for the PictBridge supporting printer on This also makes printing by the PictBridge supporting printer possible When this mode is selected the function to capture the image on the PC by connecting the PC to the US...

Page 50: ...adjust the color and contrast 2 1 1 Simple Photography Easy Menu Select the EASY tab on the CAM menu and the Easy menu will be displayed The Easy menu allows you to photograph an image easily by only selecting the mode that suits the subject Configuration of the Easy menu Biological specimen scene mode Industrial specimen scene mode NOTE focus button and exposure start button are displayed only wh...

Page 51: ...ethod of observation 2 Adjust the white balance Prepare an evenly white subject and adjust the white balance with WB 3 Adjust the focus Adjust the image composition and the focus 4 Correct exposure Adjust the brightness of the image with CMP 00 5 Confirm the image Press freeze button to confirm the image During DF FL mode always use the exposure start button 6 Save the image Save the image with Ca...

Page 52: ... parts are expressed as transparent This mode can also be used for ceramic and plastic specimens Circuit Board This mode is suitable for photographing mounting defects on a part mounted circuit board Clipping of highlights in component leads and solder joints are suppressed allowing for defects to be easily detected This mode is also suitable for high contrast subjects such as gears and other meta...

Page 53: ... adjust the white balance in the same way by using a white subject For fluorescent photographing we recommend that the white balance be adjusted under normal light conditions before photographing an image If the screen is excessively dark or if clipping occurs adjust the luminous energy of the light source or the iris diaphragm or use an ND filter to obtain the appropriate white balance NOTE For e...

Page 54: ...Saving an Image to a CF Card REC Menu or 4 6 3 Saving Image Files in the FTP Server in Chapter VI for details on how to use the REC menu 2 1 2 Special Photography on the Easy Menu DF FL Scene Mode In DF FL scene mode the camera is operated differently from other scene modes Press the DF FL scene mode button and you will enter high sensitivity display mode for focus adjustment At the same time focu...

Page 55: ...r FL fluorescent microscopy For the setting for each observation method refer to 6 2 3 Microscope Status Display Microscopy Setup Menu MIC Info Menu in Chapter VI With this function the shutter opens by entering the DF FL scene mode in case the shutter has been closed Then when the exposure by the exposure start button is completed the shutter closes When performing the focusing again with the foc...

Page 56: ...ndicator 1 3Mp 1 3Mi 5Mi C Scan Display mode for 5M cameras 2Mfull 800 6 800 5 C Scan Display mode for 2M cameras PROG S_AE MANU F_AE Exposure mode 1 250s Shutter speed C_gn 100 Camera sensitivity camera gain Photometry area Photometry mode CMP 00 Exposure compensation WB White balance AE_L AE lock Gain Y100 Image level adjust R100 B100 Color balance adjust CHRM 00 Color density HUE 00 Hue adjust ...

Page 57: ...thod that is most suitable for the purpose of observation or the intended subject as you photograph an image 1 Choose display mode 2 Choose exposure mode 3 Choose photometry mode 4 Choose tone 5 Adjust the white balance 6 Adjust the picture quality 7 Confirm exposure information 8 Save the image 9 Save call photographing conditions Monochrome cameras No color settings are displayed ...

Page 58: ...subject in the shortest time possible 1 3Mi 800 6 Ex Fluorescent photography nighttime photography with a lens Photographing a bright subject with priority placed on focusing C Scan 2 Choosing exposure mode Choose your desired exposure mode from PROG S_AE MANU or F_AE Normally PROG should be selected Sample settings for different uses are described below Try these settings before determining your ...

Page 59: ...ANU if you are always photographing under the same exposure conditions or photographing special subjects In this mode the electronic shutter speed or the camera sensitivity is manually set in the Advn menu The electronic shutter speed can be set from 1 1 000 s to 60 s for the non cooled camera and 1 1 000 to 600 s for the cooled camera The camera sensitivity can be set from x1 C_gn 100 to x46 C_gn...

Page 60: ...t field Average photometry Whites on the background need to be whiter Peak hold photometry with exposure correction added The background is mostly black and the subject is sporadically bright Peak hold photometry Selecting a photometry area Use one of the photometry area icons to specify a range of the screen in which you want the exposure to be adjusted Normally a wide photometry range is recomme...

Page 61: ...he image will have clearly distinguishable darks and brights Recommended example Cells and tissue general subjects TONE5 Linear There is a linear output proportional to the input from the camera Use this tone when you want to examine the difference in luminous energy from photographed data Recommended example DIC observation PH observation etc TONE6 Provided for metal tissue Use this tone when you...

Page 62: ...hile you adjust the picture quality as necessary Sharpness SHP0 to SHP5 A sharp video image will be obtained by enhancing the contours of the image The sharpness can be adjusted in six steps from 0 off to 5 strong Noise reduction NR ON NR OFF Selecting NR ON helps to reduce noise on the screen resulting in a clear image Lag or tails on moving objects may occur when NR ON is selected When photograp...

Page 63: ...subject 3 Adjust the amount of incident light so that while using AE or MANU exposure the shutter speed is in the range of about 1 7 5 to 1 250 seconds 4 Press REG to enter the Registration menu 5 Press SD Set If shading correction has been set normally you will see 1 2 Save displayed on the screen Select the number in which you want to save your settings and press Save to save Note that shading c...

Page 64: ...values of each color are shown in numeric form at the right edge and shown by levels at the bottom How to read and set exposure levels If the brightness distribution is concentrated on the right edge of the histogram it means that the highlighted parts of the screen are saturated Conversely if the brightness distribution concentrates on the left edge of the histogram it means that the low lighted ...

Page 65: ...enu in the same way as from the Easy menu In addition to scene modes custom modes can be called from c1 through c7 You also can confirm the information in the Info box In the Advn menu you can save the set values of individual subjects and objectives in each custom mode 1 From the Advn menu set conditions such as camera operation mode and exposure NOTE Press Clset to temporarily clear the set valu...

Page 66: ...d camera gain manually fine adjustment of the displayed image can be performed 6 Press exposure start button and the DS L1 starts automatic exposure When finished a screen is displayed with a frozen image is displayed when exposure time is longer than 6 seconds Right click stops photographing 7 If necessary adjust the shutter speed and camera gain manually and press exposure start button again 8 T...

Page 67: ...utter speed of the DS L1 is in the following range 5M cameras 5Mi mode 1 3 s to 1 s 1 3Mp and 1 3Mi modes 1 7 s to 1 s 2M cameras 1 15 s to 1 s Select MANU for exposure mode and set the shutter speed within the above range No noise reduction functions are supported Select NR OFF and leave it selected while in use The DS L1 does not have an automatic exposure function for stroboscope Use the automa...

Page 68: ... DS RC to save images to a CF card Before you can do this you must first select Save to Card for Capture Function in Additional Settings of the SETUP menu NOTE Refer to 2 1 1 Simple Photography Easy Menu in Chapter VI for details on how to use the CAPTURE button to save images The following describes how to save images from the REC menu Configuration of the REC menu NOTE Refer to 4 6 Saving Images...

Page 69: ... information or comments in text format up to 100 characters at the same time you save an image You may enter the subject name number and the objective magnification for example to facilitate confirmation at a later time When you save an image in BMP format the comment is saved to a file named after the image with the extension txt added When you save an image in JPEG format it is saved to a file ...

Page 70: ...in File Settings so that files will be named after the photographed date and time 1 Press 1shot to change to Continue The Cnt and Int icons will be displayed 2 In Cnt set the number of frames to be photographed Up to 500 frames can be set If Endless is selected in Cnt as the number of frames an image will be photographed successively 3 In Int set an interval time ranging from 10 seconds to 6 hours...

Page 71: ...e V or U to scroll the display up or down To use the panel switch or keyboard to scroll move the cursor to V or U and press the Enter button or key Erasing images Locate the file name you want to erase at the Z position and press erase button The image will be erased Displaying a thumbnail Locate the folder name whose thumbnail you want to display at the Z position and press thumbnail display butt...

Page 72: ... zoom to zoom in or out in either half of the screen If you are using a mouse you can scroll the display up or down on either screen 1 Select DWIN on the VIEW menu Approximately half of the middle part of the screen displayed before being split moves to the left to become a still or frozen image Any part of the screen that has disappeared as a result of this operation can be made to reappear by sc...

Page 73: ...e is enlarged or reduced as will be suitable for the printer s paper size before being printed Real 10 print mode If the Mag Fix check box in Additional Settings is selected images are printed in real 10 mode When printing in this mode always be sure to use the relay lens optional Use the x0 7 relay lens for 5M cameras and the x0 55 relay lens for 2M cameras The scaling factor in this mode is equa...

Page 74: ... real 10 printing is a scale of 10 mm on a printed image Comment Select the Append check box for Log on the REC menu and enter a comment in the input box The comment will appear on the image being printed Comments can be up to 32 characters It is convenient to record the subject name number and the objective magnification for confirmation at a later time NOTE You can use the Telnet command to sele...

Page 75: ...he TOOL menu are hidden when you turn on the electronic zoom The hidden contents can be redisplayed after you turn off the electronic zoom If the microscope connected to your system is the Nikon 80i 90i microscope with motorized or intelligent nosepiece the content drawn with the TOOL menu displayed on the screen pen count marker text two points distance measurement circular perpendicular angular ...

Page 76: ...asure Measurement functions Scale Displays a scale C Scale Displays an XY scale Cross Crosshairs Cross Display Color Changes color or shape Set Changes display mode and saves settings Superimposition Superimposition Create Create Disp Display Info Information display Clear All Erases overlay information Overlay ON OFF Displays or hides overlay Pen Pen function Marker Count marker function Text Tex...

Page 77: ... screen Overlay OFF hides the overlay from the screen Even while Overlay OFF if you press any icon to draw on the overlay e g Pen or Text the overlay will automatically reappear Erasing the overlay Press Clear All and Y N will appear asking you to confirm whether or not to erase Press Y if you want to erase or N if you do not You cannot partially erase an overlay All pen drawings count markers tex...

Page 78: ...use to use this function 1 Press Pen The tool bar shown below will be displayed 2 Press the red blue green black white or yellow color icon to determine the color of the pen 3 Click at the starting point and hold down the mouse button while you drag and the locus of the pointer will be drawn If you failed to draw use Clear to erase the locus you ve now entered In this case what you ve drawn with t...

Page 79: ...nts independently for each color Or you can return to one of the colors you previously used and continue counting from the last count of that color If you failed to mark any point you can use Undo or Redo to cancel or redo your most recent action Up to 10 recent actions can be retrieved Use Clear to erase all the content you ve now entered All markers you ve attached will be erased unless you have...

Page 80: ...CL to erase all characters or BS to erase one character To enter characters from your keyboard move the cursor to the text input window and press the Enter key Use the SHIFT Backspace keys on the keyboard to erase all characters or the Backspace key to erase one character Type your comment on the keyboard and hit the Enter key to confirm the content of your comment The Input Mode message disappear...

Page 81: ...numbers s1 through s7 and the calibration information for that number will be displayed in the Info area for your confirmation 2 Press Calib to bring up a calibration screen like the one shown below 3 Draw a calibration line about the length of a specific subject on the image that you want to be the reference Click the mouse button at the starting point of the object to be measured To draw a line ...

Page 82: ... keypad to enter the magnification of the objective 4 Click the numeric value input box to display the numeric keypad Use the keypad to enter the magnification of the relay lens 5 If you want to attach information to the calibration value you ve created click the Info input box and enter text for the information you want to attach This information will prove helpful because you can confirm Info wh...

Page 83: ...Nikon 80i 90i microscope with motorized or intelligent nosepiece note that each calibration value call number is linked to the hole number of the nosepiece This means you cannot change calibration values by selecting s1 through s7 Calibration values are automatically changed when you change the objective 3 4 3 Measuring the Distance between Two Points You can measure the distance between two speci...

Page 84: ...o or Redo recent measurement lines drawn from that point in time cannot be canceled 4 To save a measurement line to the overlay of the image press Paste to fix after drawing it 5 Press to return to the menu in which you previously were Only measurement lines cofirmed with Paste will be saved Once the content is fixed as valid you cannot erase it unless you erase the entire overlay NOTE The display...

Page 85: ... or redo your most recent action Up to 10 recent actions can be retrieved Use Clear to erase all the content you ve now entered All lines drawn before confirmation with Paste will be erased NOTE If you draw a measurement line in the middle of Undo or Redo recent measurement lines drawn from that point in time cannot be canceled To redraw a reference line select the icon once again 5 To save a meas...

Page 86: ...o nine To change colors click another color icon so angles drawn in that color will be assigned numbers separately from those in other colors To redraw an angle use Undo or Redo to cancel or redo your most recent action Up to 10 recent actions can be retrieved Use Clear to erase all the content you ve now entered All lines drawn before confirmation with Paste will be erased NOTE If you draw a meas...

Page 87: ...r to erase all the content you ve now entered All lines drawn before confirmation with Paste will be erased NOTE To measure a new circle select the icon once again If you draw a new circle in the middle of Undo or Redo recent measurement results cannot be canceled 4 To save a circle and its measurement results to the overlay of the image press Paste to fix after the measurement 5 Press to return t...

Page 88: ... crosshairs and a center only crosshairs The color can be selected from seven colors red green cyan yellow magenta white or black 5 Press Set to bring up the tool shown below This tool allows you to select a display mode for the crosshairs that suits the subject whose position you want to adjust 6 Radius is an option that displays a concentric circle along with the crosshairs with respect to its c...

Page 89: ...d images By leaving the Cross check box selected on REC menu you can draw crosshairs on saved or printed images NOTE If while crosshairs are on you create or display a superimposed image the crosshairs will be hidden The crosshairs can be redisplayed after you have finished creating or displaying a superimposed image Crosshairs display at power on The DS L1 can be set to display crosshairs when it...

Page 90: ...m 1 mm to 1000 µm etc Normally values with these functions are labeled with the units nm µm mm or cm However if calibration values are selected after inch mil conversion they are labeled mil and an E is indicated representing inch conversion If you perform an electronic zoom while using this function the screen display will reflect the zoom magnification If you change the optical zoom of the digit...

Page 91: ...NG is displayed in the Info area In that case change the calibration unit that has been set for example from 1 mm to 1000 µm Normally values with these functions are labeled with the units nm µm mm or cm However if calibration values are selected after inch mil conversion they are labeled mil and an E is indicated representing inch conversion If you perform an electronic zoom while using this func...

Page 92: ...ter into the area and click it while you drag in order to specify the range in which you want to create a superimposed image 3 Press Create and the system will start creating a superimposed image automatically This will take some time Depending on the subject the image thus created may not have sufficient contrast for easy recognition In such a case use Clear to temporarily erase the image and Lev...

Page 93: ...r of the cross hairs 3 To change line colors press Color Seven colors are available red green cyan yellow magenta white and black 4 To clear the display press C Msr once again Normally the axes for measuring vertical and horizontal distances are displayed on the screen but not printed on or saved with images To apply axes to saved images or to print axes along with images select the Cross check bo...

Page 94: ... a network Possible operations in each method are described below The symbols and in the diagrams below indicate the flow of images while the symbol indicates the flow of control Using a WEB browser to operate the DS L1 from a PC Get images from the DS L1 Control the DS L1 in a simple way A WEB browser may be used from a LAN based PC to observe images It is possible to view small images with a rap...

Page 95: ...ands may be used from a PC to achieve advanced control of the DS L1 In this case the DS L1 s Telnet server function is used Operating on a PC from the DS L1 Send images to an FTP server Images can be transferred to a specified FTP server NOTE This function cannot be used unless an FTP server facility is available on the PC side Because up to five FTP servers can be specified images can be easily a...

Page 96: ...corner of the Network Settings screen of the SETUP menu In automatic IP address acquisition by DHCP the assigned IP addresses change dynamically Accordingly if you are using a web browser to access the DS L1 from a PC we recommend setting a fixed IP address for the DS L1 Confirming the PC to be network connected OS of the PC used for a Web browser and browser versions There may be slight differenc...

Page 97: ...DS L1 If you are going to connect the DS L1 to a LAN you need to enable it for network operation and set its own address To make network settings on the DS L1 select Network Settings from the SETUP menu to call up the Network Settings screen shown below Enable Network To use a network always select this check box Read Only FTP WEB disable Write Control This is to disable writing to the internal fi...

Page 98: ...address value hold down Skip on the screen keyboard while you skip to the last entry of the address then reenter the correct value Once you have finished with all the settings press SAVE and then restart the DS L1 To revert to the initially set value press Default and then SAVE and restart the DS L1 Settings for improved security To improve security make the following settings on the Network Setti...

Page 99: ...L1 and of time information When accessed from a WEB browser access logs are kept only when the user IDs have been set in the Internal Server on the DS L1 side However these logs disappear when the DS L1 is powered off Run the Telnet command w_NLGON and the initial settings can be changed to display NETLOG To get the data contained in the logs access the DS L1 using an FTP command and get and read ...

Page 100: ...ternet Explorer 1 Start the PC Refer to your computer s user manual for details 2 Launch Internet Explorer Refer to your computer s user manual or the Internet Explorer help function for details 3 Select Tools Internet Options 4 Select Connections 5 Select LAN Settings Left click here to open the pull down menu Left click here Left click here Left click here ...

Page 101: ...P address of the DS L1 Refer to 4 2 2 Setting Essential Items in Chapter VI for details on the IP address of the DS L1 Enter a period after every three digits of the IP address If the first digits of the IP address are 00 or 0 do not enter the zeros 8 Click OK Move the pointer to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 Left click here Ent...

Page 102: ...anual for details 2 Launch Netscape Refer to your computer s user manual or the Netscape help function for details 3 Select Edit Preferences 4 Select Advanced Proxies Lift click Advanced When a menu appears under Advanced left click on Proxies Left click here Left click here to open the pull down menu and select Preferences from the menu ...

Page 103: ...ential Items in Chapter VI for details on the IP address of the DS L1 Enter a period after every three digits of the IP address If the first digits of the IP address are 00 or 0 do not enter the zeros 7 Click OK Move the pointer to the position of OK and left click on it Repeat this until the screen returns to the one from step 1 Left click here Left click here Enter the IP address here Example Wh...

Page 104: ...am image ROOM2 Allows you to load saved image files from a CF card into the PC If multiple PCs simultaneously access one DS L1 through a WEB browser operation may slow down In the case of the simultaneous access from only two units of PC the operation should be comfortable NOTE The screen shots shown in this section may differ depending on which browser software or version is used Regarding the us...

Page 105: ... Explorer or Netscape Communicator on your computer 2 Enter the IP address of the DS L1 in the URL address field Example http 192 168 10 2 The Entrance screen of the DS L1 will appear 3 Click either ROOM1 or ROOM2 ROOM1 and ROOM2 are explained in more detail in the subsequent pages Click either ROOM1 or ROOM2 ...

Page 106: ...trance screen Reload Redisplays the ROOM1 screen with the preview image updated ROOM2 Displays the ROOM2 screen 2 Stream Window Displays a stream image subwindow in which a still picture is successively displayed as a stream image To close this window click the x button at the upper right corner This window is automatically closed when you go to another screen Entrance or ROOM2 Method for displayi...

Page 107: ...ge size you want to download and the latest image in that size will be downloaded into the PC in BMP format 1 When you click any image size to select Save it to disk a screen is displayed for you to select the folder in the PC that you wish to save to 2 Choose the folder in the PC where you want to save the image file Enter a file name as necessary and click the Save button When you click the Save...

Page 108: ...reen is displayed for you to select the folder in the PC that you wish to save to When you right click on the displayed image the image can be downloaded into the PC in JPEG format If you select Save As in the displayed menu the screen shown below will be displayed 2 Select the folder in the PC where you want to save the image file Enter a file name as necessary and click the Save button When you ...

Page 109: ...ant to be enlarged or click one of the four up down left right direction buttons If you deselect the E Zoom check box the E Zoom function is turned off and the direction buttons and the sample image disappear with the preview screen displayed in normal size If high picture quality 1280 x 960 is selected for the picture quality of the preview screen a middle quality 640 x 480 image is displayed in ...

Page 110: ...d the folder at the level directly below it and the JPEG format files and BMP format files in that folder all are displayed 5 Files in the CF card Shows the file names created date time and log comments of the JPEG format files and BMP format files in the CF card The log comments displayed here are the contents of the log text that you attached from the REC menu by selecting Append when saving ima...

Page 111: ...mmand to search for a directory named RAMIBOX 3 Execute the cd RAMIBOX command The following virtual file names will be displayed IMG bmp IMG jpg Here represents dimensional values such as 1680 or 1280 indicating image size The time of day for the files displayed at this time is 0 hours 0 minutes The actually acquired files are given the time of day when you issued the Get command 4 Execute the Ge...

Page 112: ... executing Telnet commands Your organization s network may have been set up in a unique manner so invalid operations could cause a problem on your network Enter the IP address of the DS L1 from the MS DOS prompt command prompt of Windows to connect to the DS L1 Example Telnet 192 168 10 2 Commands Specify the commands listed in this manual A detailed command list is available from Nikon If you wis...

Page 113: ... L1 you need a PC which has FTP server functions Only basic settings are described here Refer to your OS s help function and other materials for more detailed information on settings 4 6 1 1 When Using Windows NT Workstation Ver 4 0 Setup procedure 1 Install Microsoft Peer Web Services Add FTP server functions 2 Add a user User ID and Password input Set the DS L1 as the login user 3 Specify the ho...

Page 114: ...iles are to be transferred Also select the Write check box to enable writing to the home directory When image files are transferred the FTP Dir specified on the DS L1 side is created at a location below this home directory and the image files are saved in that directory Example If the home directory is C DSFTP and the FTP Dir specified on the DS L1 side is FTPIBOX then the FTP transfer directory i...

Page 115: ...DSFTP DS2 and C DSFTP DS3 That way you can specify a directory separately for each user 4 6 1 2 When Using Windows 2000 Professional and XP Professional The following explains how to set FTP functions in Windows 2000 Professional and XP Professional The Control Panel is displayed in a different way on the two operating systems In Windows 2000 you select Start Settings Control Panel In Windows XP y...

Page 116: ...ssword To ensure that the same information has been set for External Server in Network Settings of the DS L1 enter the user ID and the password within ten alphanumeric characters in lower or uppercase Deselect User must change password at next login check box NOTE You also can create accounts and set passwords from Control Panel Users and Passwords or User Accounts for XP Specifying the home direc...

Page 117: ...Sites properties window Be aware that if you select the Allow Only Anonymous Logon check box no one but an anonymous user can log on When specifying a directory in which to save image files separately for each of multiple DS L1s connected 1 Select Computer Management Local System Tools Local Users and Groups Users 2 Double click the user you want to set and specify a local path for the home folder...

Page 118: ... the built in Ethernet through Apple Logo System Environment Settings Network Settings On home directory settings and the separation of save directories for each of multiple users DS L1s When a user is added a home directory is automatically created under the specified user name and directory name at a location below Macintosh HD Users When image files are transferred the FTP Dir specified on the ...

Page 119: ...rmation can also be accomplished by selecting Macintosh HD Applications Utilities Network Utility The Info tab Shows the IP address and other information The Ping tab Sends a ping command To use a function that is equivalent to the command prompt in Windows select Macintosh HD Applications Utilities Terminal to display the Terminal screen ...

Page 120: ... belongs to a different network group you must enter the gateway settings Server No Five servers can be stored in memory Assign each server a number IP Address 192 168 010 001 Input example User ID Enter a user ID that is needed to log in to the server Pass Word Enter a password that is needed to log in to the server as necessary Gate Way 192 168 128 001 Input example If any entry in the IP addres...

Page 121: ...to for reasons of security functions such as packet filter firewall or security gateway Ping test may be reexecuted after disabling the security function of the server In that case restart the DS L1 first Also even when Ping was not responded to there is no problem with the server connection only if the server can be logged in in the login test described below Login test To test logging in to the ...

Page 122: ... file is automatically assigned a file name according to settings on the DS L1 side The folder names and file names are set in File Settings of the SETUP menu For file names we recommend selecting Auto for File Name so that files will be named after the dates and times at which they are saved to the FTP server When images are sent via FTP the DS L1 does not check for duplicate file names Although ...

Page 123: ...at given intervals The following explains how to save images from the REC menu Configuration of the REC menu NOTE Refer to 2 2 1 Saving an Image to a CF Card REC Menu in Chapter VI for details on how to set in the REC menu Press CF to save an image to the CF card 1shot Only one image is saved Continue Interval photography photographed at given intervals Select the CF FTP check box and an image wil...

Page 124: ... that files will be named after the photographed date and time 1 Press 1shot to change to Continue The Cnt and Int icons will be displayed 2 In Cnt set the number of frames to be photographed Up to 500 frames can be set If Endless is selected in Cnt as the number of frames an image will be photographed successively 3 In Int set a photography interval time It can be set anywhere from 10 seconds to ...

Page 125: ...or written to correctly About the recording media used by the DS L1 Usable media Compact flash card Type I and II microdrive Media formatting When formatting media in your PC make sure it is formatted in FAT12 16 Sometimes these are expressed simply as FAT or in FAT32 Note that depending on the OS used you may not be able to select a format The DS L1 has a function for formatting media The media p...

Page 126: ...at the interface board and the DS L1 be connected in only one hub stage and not in cascaded hub stages NOTE When multiple units of DS L1 are connected to a Windows 2000 machine equipped with a USB 1 1 interface board or a USB 1 1 hub the DS L1 units do not operate normally If connecting multiple units of DS L1 is desired use a USB 2 0 interface board and a USB 2 0 hub or use Windows XP for the OS ...

Page 127: ...u have downloaded read the manual supplied with your interface board or consult the manufacturer 5 2 Connecting the DS L1 and a PC Follow the procedure described below to connect the DS L1 and a PC 1 Turn on the power to the PC main unit and start Windows 2000 or Windows XP 2 Plug the A connector of the USB cable into the USB port of the PC main unit or the hub 3 Plug the B connector of the USB ca...

Page 128: ...1 Installing the Device Driver When the DS L1 is connected to a PC for the first time the PC automatically recognizes the DS L1 and installs the device driver that comes standard with the OS During the installation process the window shown below will be displayed sequentially You do not need to perform any specific operations ...

Page 129: ...rectly into position the message Please insert media is displayed when you click the icon for the media drive A similar message is displayed when you click the icon for the CCD image drive Connecting the DS L1 will immediately disable all other USB devices connected to the PC In such a case change the drive letter assigned to the media drive or the CCD image drive The following shows how to make t...

Page 130: ...disk icon on the My Computer screen to display its Properties dialog box This dialog box helps you identify the drive assigned to that removable disk In the case of the CCD image drive for example it can be identified based on the volume label NIKONIMG and its capacity 16 0 MB as shown below NOTE Note that because this drive is write protected its volume label cannot be altered ...

Page 131: ...d from the PC to other media files in the same way as files are normally manipulated on a PC Removing media When the DS L1 is connected to a PC follow the procedure described below to remove the media from the media drive 1 Check to see that the PC card indicator on the DS L1 is turned off Close the menus displayed on the DS L1 2 If any image files have been loaded from the media into the PC close...

Page 132: ...Settings in Chapter VI NOTE To capture files at maximum resolution 2560 1920 for 5M cameras or 1600 1200 for 2M cameras you must set the correct parameters for size and the correct display mode The display mode also needs to be set to maximum resolution 5Mi for 5M cameras or 2Mfull for 2M cameras Setting the display mode to a lower resolution will result in image captures at the lower resolution D...

Page 133: ...rming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed 2 Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed ...

Page 134: ... if the PC remains powered on However to prevent data from being corrupted by removing the DS L1 during write operation follow the procedure described below 1 Eject the media from the media drive before removing the DS L1 2 Unplug the USB cable or turn off the power to the DS L1 3 When the below is displayed click OK Plug in the USB cable and turn on the DS L1 The device will be recognized by the ...

Page 135: ...ocess messages will be displayed sequentially in the task tray system tray at the lower right corner of the monitor screen indicating that new hardware has been detected You do not need to perform any specific operations When the Safely Remove Hardware icon is displayed in the task bar it means the PC has finished installing the driver When the DS L1 is connected to a USB 1 1 compliant USB port th...

Page 136: ...K button When you click OK button the Explorer screen opens showing the content of each drive NOTE In the following cases there might be a conflict between another connected device and the drive letter When media is inserted correctly into position the message Please insert media is displayed when you click the icon for the media drive A similar message is displayed when you click the icon for the...

Page 137: ...ble Storage on the My Computer screen to access the media The image files on the media drive can be retrieved from the media or transferred from the PC to other media files in the same way as files are normally manipulated on a PC Removing media 1 Check to see that the PC card indicator on the DS L1 is turned off Close the menus displayed on the DS L1 2 If any image files have been loaded from the...

Page 138: ...tings in Chapter VI NOTE To capture files at maximum resolution 2560 1920 for 5M cameras or 1600 1200 for 2M cameras you must set the correct parameters for size and the correct display mode The display mode also needs to be set to maximum resolution 5Mi for 5M cameras or 2Mfull for 2M cameras Setting the display mode to a lower resolution will result in image captures at the lower resolution Do n...

Page 139: ...rming the updated date time of image files 1 Open My Computer and display image file icons Then select View Refresh to update the image file icons displayed 2 Right click any image file icon of interest and select Properties from the ensuing menu The updated date and time will be displayed ...

Page 140: ... DS L1 during a write operation follow the procedure described below 1 Eject the media from the media drive before removing the DS L1 2 Click the Safe to Remove Hardware icon on the task bar 3 Drives should be selected one at a time First select the media drive If the media drive can be removed the message shown below is displayed 4 To remove the CCD image drive follow step 3 5 Unplug the USB cabl...

Page 141: ...croscopy setup menu MIC Info menu is not displayed Before using microscope control function from DS L1 you must register the microscope status from the PC using the setup software Consult the instruction manual for your microscope 6 2 Menu Overview When you open a menu while the microscope is connected to your system the menu displayed on the left side of the screen for default settings will have ...

Page 142: ... in longitudinal and lateral directions Show the magnification of the optical zoom Show the name of the filter Set up microscopy and show the current microscopy Precautions regarding menu display Depending on the type of microscope connected to your system not all items of the microscope control menus and microscope status display menus can be displayed The items that can be displayed on the DS L1...

Page 143: ...illumination field diaphragm No menu displayed ND filter No menu displayed Analyzer No menu displayed 2 When the Nikon 80i microscope incorporating DIH E is connected Parts Menu displayed Focus Z axis No menu displayed Stage No menu displayed Intelligent nosepiece Status display menu displayed Nosepiece Manual nosepiece No menu displayed Optical zoom Control status display menu displayed Optical p...

Page 144: ...isplayed Filter cube No menu displayed Lamp Control status display menu displayed Motorized universal condenser Control status display menu displayed Universal condenser Non motorized universal condenser No menu displayed With motorized diaphragm Control status display menu displayed Diascopic illumination aperture diaphragm Without motorized diaphragm No menu displayed Diascopic illumination fiel...

Page 145: ...atus display menu displayed Filter cube Status display menu displayed Lamp Control status display menu displayed Motorized universal condenser Control status display menu displayed Universal condenser Non motorized universal condenser No menu displayed With motorized diaphragm Control status display menu displayed Diascopic illumination aperture diaphragm Without motorized diaphragm No menu displa...

Page 146: ...nu displayed Filter cube Control status display menu displayed Lamp Control status display menu displayed Motorized universal condenser Control status display menu displayed Universal condenser Non motorized universal condenser No menu displayed With motorized diaphragm Control status display menu displayed Diascopic illumination aperture diaphragm Without motorized diaphragm No menu displayed Dia...

Page 147: ...ol menu 1 you can control the motorized units of the microscope used often Here the menus displayed on DS L1 are explained for the case in which the Nikon 90i microscope incorporating DIH E and having all motor driven parts attached full spec is connected in a microscope system Keep in mind that if any of these parts do not exist or manual parts are attached menus for these parts will not be displ...

Page 148: ...e can be moved for easier specimen replacement Rapid transfer mode Objective Shows magnification and NA value Shows detail information Selects the objective to use Optical zoom Changes the zoom magnification of the rear port of the DIH Optical path Binocular Front port Rear port Changes and shows optical path Rear port when DIH M is connected for 30 70 illumination Closed Open Opens or closes the ...

Page 149: ...nu Using the autofocus function When this function is selected focus is adjusted automatically Before using this function please read the separate manual entitled 90i Reference Autofocus Also refer to Section 6 3 Autofocus Precautions in this manual 1 Select the autofocus AF icon 2 The message Now Focusing will be displayed at the upper right part of the screen indicating that the autofocus functi...

Page 150: ...ns the stage Compatible only with Nikon 90i microscopes using a motorized stage 1 Set the coarse fine select icon to coarse mode The appearance of the arrow icons changes to indicate a setting to coarse or fine motion modes for coarse motion mode for fine motion mode 2 Use the arrow icons to adjust the stage position 3 To perform final fine adjustments set the coarse fine select icon to fine motio...

Page 151: ...on consult the instruction manual for your microscope If you are using an intelligent nosepiece only the objective magnification and NA value are displayed Selecting icon shows detailed information on the objective being used Select the X mark on the detailed information display screen to turn the detailed information display screen off Right clicking on the icon representing a nosepiece number sh...

Page 152: ...can be changed in the range x0 8 to x2 0 The current magnification is displayed on the microscope status display microscopy setup menu MIC Info menu When you change the optical zoom the episcopic illumination field diaphragm is also changed accordingly For more information refer to the instruction manual for your microscope When you change the optical zoom the overlay data excluding the XY scale a...

Page 153: ... connected to the front port when you use it select the check box by putting a check mark in it NOTE The F_Port check box is displayed only when the connected microscope incorporates the DIH M or DIH E When the DS L1 is used with the front port you will see the message None displayed in the Zoom column of the microscope control menu 1 and the microscope status display microscopy setup menu MIC Inf...

Page 154: ...amp is off select the icon to turn the lamp on 2 While the lamp remains lit the icon is marked with a red border To turn the lamp off 1 When the lamp is on select the icon to turn the lamp off 2 While the lamp remains turned off the icon is marked with a red border Adjusting the brightness of the lamp 1 When the lamp is lit select the icon and the lamp will become brighter in increments of 0 1 V C...

Page 155: ...ents are missing or manual parts are attached no menus will be displayed for those components Menu configuration when the microscope connected has all motorized parts attached Episcopic illumination field diaphragm Adjusts the diaphragm Condenser Shows condenser module name Selects the condenser module you want to use Diascopic illumination aperture diaphragm Adjusts the diaphragm Diascopic illumi...

Page 156: ...condenser from within this display to change condensers Diascopic illumination aperture diaphragm Adjusts the diascopic illumination aperture diaphragm Compatible only with the Nikon 90i microscope 1 In the diascopic illumination aperture diaphragm column select the icon to open the diaphragm in increments of 0 1 Or select the icon to close the diaphragm in decrements of 0 1 2 Briefly depress the ...

Page 157: ...r Adjusts the transmission factor of the ND filter Compatible only with Nikon 90i microscopes with motorized ND filters attached 1 In the ND filter column select the icon to increase the transmission factor by 3 relative to the current set value Or select the icon to reduce the transmission factor by 3 relative to the currently set value 2 Briefly depress the mouse button to change the transmissio...

Page 158: ...n when connected to the rear port of the DIH Info box information display indicates operating status or detailed items for the selected icon Stage position Z axis Shows address along the vertical axis Z axis Stage position X axis Shows address along lateral axis X axis Stage position Y axis Shows address along longitudinal axis Y axis Observation method Sets the microscope for bright field microsc...

Page 159: ...s along vertical axis Compatible only with the Nikon 90i microscope 1 The menu indicates the height of the current Z focus position i e position along the Z axis NOTE Use microscope control menu 1 to adjust focus Stage position X axis Shows address along lateral axis Compatible only with Nikon 90i microscopes connected to a motorized stage 1 The menu indicates the current lateral stage position po...

Page 160: ...tes the name of the current filter in the filter column NOTE Before using this function you must set up filter information from a PC with the setup software For more information refer to the instruction manual for your microscope Use microscope control menu 1 to change filters Observation method Sets the observation method If the microscope connected is a Nikon 90i microscope incorporating the DIH...

Page 161: ...icons representing those numbers will display the name of the observation method under the cursor in the Info box To link the observation method with the photographing mode of DS L1 1 Right click on the icon indicating an observation method to open the menu showing all observation methods 2 Enter a check mark in the check box 6 2 4 Ergo Controller button information display menu To view button ass...

Page 162: ...icroscope is connected The system will not function properly if nothing is connected or if instruments other than the modular microscope are connected 1 Use a USB 232c adapter to connect a modular microscope to the DS L1 2 Turn on the modular microscope then turn on the DS L1 3 To switch to a certain nosepiece open the menu and select the corresponding number 4 After switching nosepieces confirm t...

Page 163: ...8037 Cover glass Complies with JIS R3702 or ISO 8255 JIS R3702 Thickness 0 12 0 17 mm for No 1 0 15 0 18 mm for No 1 S ISO 8255 Thickness 0 13 0 17 mm for No 1 0 15 0 17 mm for No 1 H 2 The autofocus function may force contact between the sample and the tip of the objective resulting in damage to both For samples like the ones shown below adjust focus with the focusing handle rather than relying o...

Page 164: ...e ones indicated below Low contrast samples Samples densely dyed dark over the entire view field Samples prepared for methods other than bright field microscopy Samples that lack light and dark patterns running horizontally along the screen lateral direction in the diagram shown at right 2 Since autofocus responds to the highest contrast part of the sample the focused position of the sample may no...

Page 165: ... conditions set on the DS L1 when the sample is nearly in focus Scene mode BF Exposure time Under AE setting the exposure time should come within 1 120 to 1 350 in 1 3 MP display mode when the sample is nearly in focus Adjust with ND filters etc Warn Exp Under Microscopy condition is too dark Focusing stops at the original position for the autofocus Use the autofocus function under following condi...

Page 166: ... on the front of the CCD If you do touch the glass filter or if dirt cannot be removed from it contact your nearest Nikon representative and have them clean it Do not use organic solvents e g alcohol ether or paint thinner to clean the coated plastic or printed parts Doing so could result in discoloration or in separation of the printing 2 Storage Turn off the power when the system is not in use I...

Page 167: ...ome on Make sure the power cord is plugged firmly into an AC outlet at one end and the DS AC adapter and then the DC IN connector on the DS L1 main unit on the other Use the power cord supplied with the system Turn on the POWER switch The main unit is hot to the touch The main unit temperature may increase when used in a hot or confined space If the high temperature is accompanied by a burnt smell...

Page 168: ...Monitor in Chapter VI Only a crossline image appears on a white screen Connect the camera cable securely The image is out of focus Set Sharpness on the CAM menu appropriately See 2 Photographing Images in Chapter VI Turn off the electronic zoom function Focus the microscope or lens correctly Do not use the system in a vibration prone place Adjust the aperture diaphragm correctly See Chapter V Micr...

Page 169: ... that it circumscribes the viewfield Adjust the size of the aperture diaphragm to approximately 70 to 80 of the numerical aperture of the objective See Chapter V Microscope Adjustment Use an appropriate filter When using a phase contrast microscope or an interference microscope two luminous fluxes or multiple luminous fluxes insert the specified filter With a black and white image contrast is impr...

Page 170: ... or S2M DS 2M in File Settings of the SETUP menu See 1 4 2 Image File Settings in Chapter VI 4 Abnormal Behavior Symptom Corrective action The system s internal clock does not keep time correctly If the system is not used for an extended period of time the backup function deteriorates causing the clock to stop Simply reset the correct time The backup function recovers when the system is powered on...

Page 171: ...that crossed cables are used Checking hub connections See the instruction manual supplied with the hub for details and follow the description written in it Do not connect the DS L1 to the Uplink Be aware that Uplink on some hubs is indicated as MDI Uplink 1 1 1 or 8 8 Also if a hub has cross and straight ports caution must be exercised There is a limited number of useful ports on a hub Be careful ...

Page 172: ... returned the presence of the DS L1 is not recognized Check the cables hub connections and the network settings of the PC e g the IP address Also check that the PC is connected to a LAN by using a browser or the ping command Browser Cannot access the camera from a browser Set exception items in the browser s proxy settings Cannot control the camera from a browser Deselect the Read Only check box i...

Page 173: ...heck the network cable and hub connections Select the Enable FTP Client check box in Network Settings of the SETUP menu Even though the FTP send button is pressed the timer icon keeps running for about 1 minute and no images are sent This is because the IP address of the External Server that has been set in Network Settings of the SETUP menu cannot be found Check the IP address of the server one m...

Page 174: ...luded with the USB board does not work properly When images are successively downloaded it is the same image that appears on PC Images are updated once every 5 seconds Be aware that if files are loaded into a PC at intervals shorter than 5 seconds the captured image may not always be the latest one Warnings are displayed on the PC A warning in the task tray system tray such as HIGHT SPEED USB Devi...

Page 175: ...ted in the Additional Settings screen in the SETUP menu Unable to click the printing icon Confirm that a PictBridge supporting printer is connected to the USB D connector on DS L1 Confirm that the power for the PictBridge supporting printer is turned on In the case that it is still unable to click the printing icon even though the power is turned on close the menu turn on and off the power once mo...

Page 176: ...heck whether the Nikon 80i or 90i microscope indicates an abnormal state by buzzer or LED display In the event of any abnormality on the microscope consult the instruction manual for your microscope Unable to display microscope status Or incorrect status information is displayed Make sure the microscope initial setting has been performed Change the registration if the objective or filter has been ...

Page 177: ...c or DS 2MBWc White balance Set method color balance adjustment Color correction Contrast black level saturation hue adjustment monochrome inversion other special effects are selectable Image correction Shading correction noise reduction horizontal vertical inversion of image Operation Scene mode Selectable from a total of six biological and industrial modes resetting possible Custom setting Seven...

Page 178: ...Bridge supporting printer connectable Network IEEE802 3 compliant 10BASE T 100BASE TX DHCP supported HTTP server Telnet server FTP server FTP client General External dimensions 203 W 202 H 73 D mm not including protrusions Weight 1 3 kg Rated input voltage 12 VDC 5 Power consumption Approx 138 VA Structure of fixed part VESA 75mm standard 75 mm pitch M4 7 fixing screw holes 4 locations UL1678 suit...

Page 179: ...80 Quantization 12 bits Sensitivity Equivalent to ISO 100 Equivalent to ISO 350 Equivalent to ISO 64 with settings of Tone 3 and x1 camera sensitivity Lens mount C mount Usable lens Protruding no more than 10 mm from the mount surface into the camera Image circle 8 9 mm in diameter Image circle 11 mm in diameter External dimensions 91 W x 76 D x 41 H mm Weight 260 g 290 g 260 g 290 g Fixing tripod...

Page 180: ...15B Class A standard This product satisfies the EU EMC Directive This product satisfies the EU Low Voltage Directive This equipment has been tested and found to comply with the specifications for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environ...