Chapter 2
Individual Operations
40
Chapter 2
Individual
Operations
4
Adjusting the Diopter
The diopter adjustment ring on an eyepiece can be adjusted to match the eyesight of your right and left eyes.
A properly adjusted diopter compensates for differences in visual acuity between the right and left eyes of a person,
making binocular observation easier. It also minimizes focal deviations when switching magnification, optimizing the
performance of the objective.
Adjust the diopter settings for both eyepieces.
Notch on eyepiece
The eyepiece has a notch to prevent the rotation. When attaching, match the notch with the protrusion on the eyepiece
sleeve. Otherwise, eyepiece is not attached to the correct position.
(1) Turn the diopter adjustment ring on the right and left
eyepieces to align the end face of the diopter adjustment ring
with the line. (This is the diopter adjustment reference
position.)
(2) Follow Steps 1 through 6 in Chapter 1 “1.2 Bright-field
Microscopy” to focus on the specimen using the 10x
objective.
(3) Turn the nosepiece to bring the 40x objective into the optical
path, and turn the coarse focus knob and then the fine focus
knob to focus on the specimen.
(4) Bring the 10x (or 4x) objective into the optical path.
(5) Look into the left eyepiece with your left eye. Without
touching the focus knob, focus on the specimen by turning
the left diopter adjustment ring.
(6) Look into the right eyepiece with your right eye without
touching the focus knob to focus on the specimen by turning
the right diopter adjustment ring.
(7) Repeat Steps (3) through (6) to make sure the focus has
been adjusted properly.
Reference position for diopter adjustment
T
O
RQ
UE
CLAMP
POWER
0
90
80
70
60
50
40
30
20
10
Adjusting the Diopter
(1)
Line
(4)
Select 10x or 4x.
(6)
Also look into the right
eyepiece with your right eye
to focus with the right
diopter adjustment ring.
(5)
Look into the left eyepiece
with your left eye to focus
with the left diopter
adjustment ring.
Use the focus knob
to focus.
(3)
Select 40x.
