39
III. Operation of Each Part
15
DIC Slider
For DIC microscopy, use the DIC slider together with
the polarizer and analyzer sliders.
Attaching/removing the DIC slider
Use a hexagonal screwdriver to loosen the DIC slider
limit screw on the nosepiece.
Insert the DIC slider into the slot on the nosepiece
and screw in the DIC slider limit screw.
When removing the DIC slider from the nosepiece,
fully loosen the DIC slider limit screw using a
hexagon screwdriver, and then pull out the slider.
Placing the DIC prism in the optical path
Push in the slider to the second clickstop position to place the DIC prism in the optical path.
Removing the DIC prism out of the optical path
Pull out the slider to the first clickstop position to remove the DIC prism out of the optical path.
Selecting the DIC prism position
The correct position of the prism selection knob is indicated on the
objective barrel after the magnification and the objective N.A. indications.
In the objective shown in the figure on the right, the letter “A” on the
objective indicates that the correct DIC prism position for this objective is
“A”. Thus, when you use this objective, turn the prism selection knob on
the DIC slider to match the letter “A” with the white circle.
Selecting an interference color
Turn the prism movement knob to change the interference colors continuously.
JAPAN
L-DIC
6
1
1
0
8
A
B
Prism movement knob
Sliding limit groove
Prism selection knob
DIC slider limit screw
10X/
0.30
A
/0 BD DIC
CF Plan
Interference color
Dark
Gray
Sensitive red-violet
Characteristics
Observation similar to dark-field microscopy can be performed.
This color enables observation of the phase difference distribution for the
whole sample.
Observation with the highest color contrast can be performed.
