Nikon eclipse lv150, Instruction Manual

Page 1: ...Industrial Microscope ECLIPSE LV150 LV150A Instructions M350 E 05 4 NF 1 ...

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Page 3: ... or disregard of the instructions can cause personal injury or property damage For your safety read this instruction manual carefully and thoroughly before using the instrument Do not discard this manual but keep it near the product for easy reference In this manual safety instructions are indicated with the symbols shown below Be sure to follow the instructions indicated with these symbols to ens...

Page 4: ...V 50 60 Hz When connecting the instrument to a power line check that the line conforms to the voltage and frequency ratings mentioned above Use of a power line that does not satisfy the ratings may lead to equipment malfunction or damage or a fire 5 Power cord Use only the supplied power cord Using the wrong power cord could result in damage or a fire Also connect the microscope to a PE protective...

Page 5: ...or fire 10 Ultraviolet light from a light source other than the specified ones If you use a light source other than the specified ones and that has a mercury lamp or so on the light source radiates ultraviolet light that is harmful to the eyes and skin from the emission port Direct viewing of light from these lamps may result in snow blindness at a light case or blindness at worst To prevent injur...

Page 6: ...icroscope with a surrounding clear area of 10 cm or more Choose a location that is free from dust or dirt Choose a flat surface with little vibration Choose a sturdy desk or table that is able to bear the weight of the instrument Do not install the microscope in a hot or humid location Select a layout that allows easy removal of the power cord from the microscope s AC inlet in the event of an emer...

Page 7: ...he used lamp as special industrial waste or dispose of it according to the local regulations and rules 8 Handing of filter cubes When using the microscope configured with the illuminator LV UEPI2 a filter cube can be attached to enable epi fl microscopy Note the following precautions for handling a filter cube Interference filters in particular excitation filters exposed to intense light are subje...

Page 8: ...Center of gravity position 168 305 100 643 Stage area 503 Stage area 250 570 4 80 192 6 Eye point 484 230 230 508 5 262 79 250 Dimensions are in mm This illustration depicts the LV150A microscope configured with the LV UEPI illuminator LV TI3 eyepiece tube LV LH50PC lamphouse and 6x6 stage ...

Page 9: ...05 The height of the seating surface 1244 The height of the eyepiece height point 565 The height of the seating surface 673 760 The 95th percentile male Height 189 5 cm The 5th percentile female Height 147 5 cm The height of the eye point is that when one eye level riser is mounted on the microscope Take at least 610 mm of horizontal clearance for your legs Take at least 610 mm of horizontal clear...

Page 10: ...copy 22 5 Sensitive Polarization Microscopy 24 6 Epi Fluorescence Microscopy 25 Operation of Each Part 26 1 Operation of the Illumination 26 2 Filters 26 3 Coarse Fine Focus Knobs 27 4 Eyepiece Tube 29 5 Diopter Adjustment 30 6 Interpupillary Distance Adjustment 30 7 Field Diaphragm 31 8 Aperture Diaphragm 32 9 Illumination Selection Lever and Microcopy Selection Knob 33 10 Stage 34 11 Motorized N...

Page 11: ...es 56 9 Attaching Eye Level Riser 57 10 Attaching Column Riser 57 11 Connecting the Power Cord 58 12 Connecting the RS 232C 58 13 Installing Separately Sold Accessories 58 14 Anti static Treatment 58 15 Countermeasures for Earthquakes 59 External Communications Control 60 Troubleshooting 66 1 Viewing and control systems 66 2 Electrical 69 Care and Maintenance 70 1 Cleaning Lenses and Filters 71 2 ...

Page 12: ... Filter sliders Vertical tube Binocular part Main body of the microscope Stage Power indicator Illuminator LV UEPI Lamphouse DIC slider 2 Tool holders Analyzer slider 1 Polarizer slider 1 Objective Nosepiece Eyepiece tube LV TI3 CAUTION for heat symbol This drawing depicts the ECLIPSE LV150A microscope configured with the LV UEPI illuminator LV TI3 eyepiece tube LV LH50PC lamphouse 6x6 stage and a...

Page 13: ... movement lever Analyzer slider 1 Eyepiece DIC slider 2 Coarse focus knob Fine focus knob Fine focus knob Coarse focus stopper ring Stage fine movement knob for Y axis Stage fine movement knob for X axis Filter sliders Prism movement knob Prism selection knob Nosepiece rotation button on LV150A only Rotates the nosepiece counterclockwise when seen from above the microscope Nosepiece rotation butto...

Page 14: ...6x6 stage and attachments for DIC microscopy Names of Parts 2 When Configured with the Illuminator LV UEPI2 6 x 6 S T A G E J A P A N A STOP F STOP J A P A N FL1 S FL2 BF DF 100 20 0 100 IN OUT LV TT2 Vertical tube Binocular part Main body of the microscope Stage Power indicator Ultraviolet light shield Microscopy selection knob Illuminator LV UEPI2 Lamphouse Tool holders Analyzer slider 1 Polariz...

Page 15: ...selection knob Diopter adjustment ring Optical path selection lever Aperture diaphragm open close lever Aperture diaphragm centering screw on both sides Field diaphragm centering screw on both sides Field diaphragm open close lever Stage coarse fine movement selection switch Stage coarse movement lever Eyepiece DIC slider 2 Fine focus knob Coarse focus stopper ring Stage fine movement knob for Y a...

Page 16: ...ait for at least 30 minutes after turning off the lamp Use 12V50W HALOGEN lamp only 1 2 3 LV LH50PC JAPAN 6 5 2 7 0 1 LAMP DC12V 50W ECLIPSE LV150A 100 240V 1 2A 50 60Hz MADE IN JAPAN including interference that may cause undesired operation 5 1 0 0 0 1 This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numérique de la classe A est confirme à la norme NMB 003 du Canada SEM...

Page 17: ...piece The standard sextuple nosepiece cannot be used for dark field microscopy Polarizer Analyzer DIC slider Universal quintuple nosepiece or motorized universal quintuple nosepiece Objectives marked LU Objectives marked LU are suitable for DIC microscopy Polarizer Analyzer Polarizer Lambda plate Analyzer Filter cube Up to two cubes can be attached Fluorescence excitation light balance filter opti...

Page 18: ...nd try to focus on the rim of the diaphragm image When the rim is in focus the sample is in focus just as well Adjust the brightness Brightness control knob p 26 Lower the stage as far as it will go Coarse focus knob p 27 Adjust the brightness Brightness control knob p 26 Adjust the brightness ND filter p 26 Image of field diaphragm Viewfield Objective s pupil Image of aperture diaphragm Adjust to...

Page 19: ... fully open the field and aperture diaphragms p 31 and p 32 To compensate color temperature p 26 Select the desired magnification On the LV150A use the nosepiece rotation switch p 35 Finely adjust the focus Coarse fine focus knob p 27 Push in Binocular eyepiece 100 P 29 Turn the microscopy selection knob BF bright field P 33 2 Set the microscope for bright field microscopy If accessories for DIC o...

Page 20: ...d automatically However the lever positions are not changed The field and aperture diaphragms automati cally return to what they were before the micro scope was set to dark field microscopy Push in BF bright field p 33 1 Mount BD objectives and a BD quintuple nosepiece universal quintuple nosepiece or motorized universal quintuple nosepiece p 56 and 49 The standard sextuple nosepiece cannot be use...

Page 21: ...re fully opened automatically However the lever positions are not changed The field and aperture diaphragms automatically return to what they were before the microscope was set to dark field microscopy Adjust the brightness Brightness control knob p 26 Adjust the brightness Brightness control knob p 26 1 Mount BD objectives and a BD quintuple nosepiece universal quintuple nosepiece or motorized un...

Page 22: ...microscope to the bright field microscopy Adjust the brightness ND filter p 26 Adjust the brightness ND filter p 26 Adjust the brightness Brightness control knob p 26 Adjust the brightness Brightness control knob p 26 Push in Analyzer p 38 Push in DIC slider p 39 Push in and align the marks Polarizer p 36 Select the desired magnification On the LV150A use the nosepiece rotation buttons p 35 Select...

Page 23: ... The DIC slider can be operated to enable various microscopies including sensitive DIC Push in and align the marks Polarizer p 36 Crossed Nicols position 1 Mount objectives marked LU universal quintuple nosepiece polarizer or motorized quintuple nosepiece polarizer analyzer and DIC slider p 36 38 39 49 and 56 Push in Analyzer p 38 Select A or B Match with the objective indication Rotate the inner ...

Page 24: ...mple with bright field microscopy p 16 4 Return the microscope to bright field microscopy Adjust the brightness ND filter P 26 Adjust the brightness ND filter P 26 Adjust the brightness Brightness control knob P 26 Adjust the brightness Brightness control knob P 26 3 Set the microscope for simplified polarization microscopy Push in Analyzer P 38 Push in and align the marks Polarizer P 36 Pull out ...

Page 25: ...2 Focus on the sample with bright field microscopy p 17 4 Return the microscope to bright field microscopy Adjust the brightness ND filter p 26 Adjust the brightness ND filter p 26 Adjust the brightness Brightness control knob p 26 Adjust the brightness Brightness control knob p 26 3 Set the microscope for simplified polarization microscopy Push in Analyzer p 38 Pull out Analyzer p 38 Push in and ...

Page 26: ...y p 17 4 Return the microscope to bright field microscopy Adjust the brightness ND filter p 26 Adjust the brightness ND filter p 26 Adjust the brightness Brightness control knob p 26 Adjust the brightness Brightness control knob p 26 3 Set the microscope for sensitivity polarization microscopy Push in Analyzer p 38 Pull out Lambda plate p 37 Pull out Analyzer p 38 Pull out Polarizer p 36 Push in a...

Page 27: ...closes the optical path of illumination To prevent fading of the sample be sure to close the shutter when moving your eyes away from the binocular part Information During the epi fluorescence microscopy the UV filter is removed from the optical path But the UV filter is inserted into the optical path during the bright field or dark field microscopy The inserting removing of the UV filter is perfor...

Page 28: ...e external light source or the ND filters on the microscope Turning on off the lamp The illumination can be turned on off by the switch of brightness control knob The halogen lamp is turned off when the brightness control knob is rotated to the far side counter clockwise direction and set to the OFF position Power indicator The power indicator color changes according to the halogen lamp status Whe...

Page 29: ...SD plate the stage vertical movement range is 11 5 mm up and 28 5 mm down Never attempt either of the following actions as these will damage the microscope Rotating the left and right knobs in opposite directions at the same time Continuing to rotate the coarse focus knob after the stage has reached the limit of its motion Adjusting the torque of the coarse focus knob The torque of the coarse focu...

Page 30: ... cannot be turned any further If the coarse focus stopper is not being used be sure to turn the ring in the direction opposite to the arrow on the microscope base as far as it goes Example usage With the sample in focus turn the coarse focus stopper ring as far as it goes in the direction of the arrow labeled CLAMP on the microscope base about 3 4 revolution The coarse focus stopper is now clamped...

Page 31: ...ar part Vertical tube Light proportion 100 IN 0 20 OUT 80 Eyepiece tube LV TI3 Eyepiece tube LV TT2 100 20 0 100 IN OUT LV TT 2 100 0 0 100 IN OUT Cap Eyepiece tube LV TI3 Eyepiece tube LV TT2 Clamp screw Vertical tube adapter Cap Clamp screw Vertical tube adapter Angular adjustment of binocular part for the LV TT2 only With the trinocular eyepiece tube LV TT2 the angle of the binocular part can b...

Page 32: ...he right eyepiece not the coarse fine focus knobs Look through the left eyepiece with your left eye and the right eyepiece with your right eye to focus on the sample with the diopter adjustment rings 6 Repeat steps 3 to 5 with the 50x and 5x or 10x objectives until the image stays in focus even though the objective magnification is changed F STOP A STOP BF DF 100 0 0 100 IN OUT Engraved line Diopt...

Page 33: ...tographed area Be sure to adjust the field diaphragm after centering it Centering the field diaphragm 1 Focus on the sample with the 10x objective by following the steps of bright field microscopy p 16 17 2 Lower the field diaphragm open close lever to reduce the field diaphragm opening 3 Turn the two field diaphragm centering screws on both sides to move the center of the field diaphragm image to...

Page 34: ...inator LV UEPI2 is used the aperture diaphragm centering can be adjusted through these steps 1 Focus on the sample with the 10x objective by following the steps of bright field microscopy p 16 17 2 Remove one of the eyepieces Check that the aperture diaphragm image is seen within the objective s exit pupil in the eyepiece tube 3 Lower the aperture diaphragm open close lever to reduce the field dia...

Page 35: ...ht field microscopy It is used also for differential interference contrast DIC microscopy and simplified sensitive polarization microscopies The UV filter enters into the optical path when the BF position is selected Dark field microscopy Setting the knob to DF selects the dark field illumination so that the aperture diaphragm and field diaphragm automatically open fully The positions of the diaph...

Page 36: ...ode The fine mode Release the stage coarse fine movement selection switch The stage is now in the fine mode Turn the stage fine movement knobs to move the stage minutely in both X and Y directions 2 3x2 stage Stage movement To move the stage turn the stage fine movement knobs for the X axis and Y axis Upper knob is for the Y axis and lower knob is for the X axis Use these knobs to move the specime...

Page 37: ...epiece rotation buttons Be careful about the following items to use the motorized nosepiece To mount objectives onto the motorized nosepiece magnifications of objectives are placed in ascending order from No 1 position of the nosepiece The nosepiece rotation from No 1 position to No 5 position and from No 5 position to No 1 position is prohibited in normal operation This limit is applied because a...

Page 38: ...entation of the polarizer by turning the polarizer rotating ring Removing the polarizer out of the optical path With the polarizer placed in the optical path pull it out in the right direction to the first clickstop position The polarizer has been removed out of the optical path instead the empty hole is now in the optical path Adjusting the orientation of the polarizer Turning the polarizer rotat...

Page 39: ...er with the polarizer slider and analyzer slider to perform sensitive polarization microscopy Placing the lambda plate in the optical path Remove the dummy slider found in front of the polarizer slider and in its place insert the lambda plate slider p 51 Pushing the lambda plate slider in to the first clickstop position inserts the empty hole into the optical path Pushing it further in to the seco...

Page 40: ... at the right side of the illuminator Insert the analyzer slider into the horizontal slot with its marking facing up p 51 Insertion to the optical path Pushing the analyzer slider in to the first clickstop position inserts the empty hole into the optical path Pushing it further in to the second clickstop position inserts the analyzer into the optical path The orientation of the analyzer is as indi...

Page 41: ...ing the DIC prism position The correct position of the prism selection knob is indicated on the objective barrel after the magnification and the objective N A indications In the objective shown in the figure on the right the letter A on the objective indicates that the correct DIC prism position for this objective is A Thus when you use this objective turn the prism selection knob on the DIC slide...

Page 42: ...oscope should have been tested by a safety certification organization Selecting an excitation EX filter The excitation filter selectively transmits only the light of the wavelength range required for the sample to fluoresce while blocking the other light The wavelength range of light that can pass through the filter is called the bandwidth The bandwidth of an excitation filter determines the brigh...

Page 43: ...ed more often than before 2 LP filters are used for samples stained in multiple colors where fluorescence images for all the colors are desired However the usual combination of a dichroic mirror an excitation mirror and a barrier filter of LP filter type may not be sufficient to excite a stain that emits fluorescence of longer wavelength for example TRIC when the sample is stained with FITC and TR...

Page 44: ... filter and secure it in reverse order Check and see the arrow mark on the rim of the barrier filter is directed to downward dichroic mirror side when attaching the barrier filter If a filter made by other manufacturer is used check and see the indication on the rim of the filter Replacing the dichroic mirror The dichroic mirror is fixed with a flat spring and a mounting part inside the filter cub...

Page 45: ...ion light balancer usage Remove the vertically oriented cover on the left side of the illuminator and insert the excitation light balancer with its indication faces back When the excitation light balancer is inserted to the limit position it enters into the optical path You can adjust the excitation light by sliding the excitation light balancer horizontally Objectives To use the excitation light ...

Page 46: ...avelength Effective diameter on the aperture diaphragm surface 50 The transmittance for the FITC is designed to keep approximately 100 because the FITC is usually dark fluorescent image Optical path position 1 Between 1 and 2 2 Between 2 and 3 3 TRITC Texas Red 0 Variable 0 to 50 50 Variable 50 to 100 100 DAPI 100 Variable 100 to 50 50 Variable 50 to 0 0 FITC 100 100 100 100 100 ...

Page 47: ...ed under unsuitable conditions When selecting the installation location note the following Avoid a brightly lit location such as exposed to direct sunlight or directly under a room light The image quality deteriorates if there is excessive ambient light Choose a location that is free from considerable dust or dirt Choose a flat surface with little vibration Choose a sturdy desk or table that is ab...

Page 48: ...rious filters LV UEPI illuminator LV TI3 eyepiece tube Dummy slider Analyzer slider Dummy slider or Analyzer slider Filter sliders x2 Lamphouse Lamphouse 12V 50W halogen lamp Various filters Fiber adapter Light guide fiber or Light source Eyepiece Dummy slider Polarizer slider Filter slider x2 Analyzer slider Filter cube Two cubes max Compensation filter attached with the fiber adapter LV UEPI2 il...

Page 49: ...stage made by other manufacturer can be attached by using the sub stage 2 The tap dimensions for the sub stage 2 are described in the figure on the right 1 Lower the sub stage completely with the coarse focus knob 2 Loosen the clamp screw for the sub stage 2 And then attach the sub stage 2 with fitting the dovetail joints on the up down part Secure the sub stage 2 by the clamp screw so that the up...

Page 50: ...oo much 3x2 stage 1 Lower the sub stage completely with the coarse focus knob 2 Place the sub stage and secure it with the four M4 screws provided with the sub stage using the 3 mm hexagonal wrench 3 Loosen sufficiently the stage clamp screw Place the holder on top of the stage and fit it in position so that it is level Tighten the clamp screws Take care not to lift up the holder by tightening the...

Page 51: ...to the groove in the bottom of the microscope arm and push it all the way Pass the signal cable of the nosepiece through the bottom hole of the arm into the microscope 4 Secure the nosepiece by tightening its clamp screw 5 Connect the signal cable of the nosepiece to the cable in the arm 6 Close the cover over the connection block and secure it with the two M4 screws Nosepiece clamp screw 2 1 4 5 ...

Page 52: ...h the illuminator 5 For the LV UEPI2 attach the ultraviolet light shield to the front bottom of the illuminator using the two screws supplied Ultraviolet light shield Harmful light or strong light may be emitted from objectives with some excitation methods Be sure to attach the ultraviolet light shield to the LV UEPI2 Be sure to use the attached screws to fix the ultraviolet light shield If other ...

Page 53: ...zer slider 3 Filter sliders and filters 1 Remove each filter slider from the illuminator There are two sliders 2 Pull out the locking plate from the filter slider 3 Insert the desired filter Two filters can be set on the filter sliders 4 Reinstall the locking plate 5 Affix the label to the appropriate lug of the filter slider 6 Attach the filter sliders to the illuminator Filter B label Empty Filt...

Page 54: ...s of excitation method supplied with the illuminator and find the one that corresponds to the filter cube just installed Affix it to the position FL1 or FL2 on the microscopy selection knob If there is no sticker corresponding to the excitation method of the filter cube write the excitation method in a blank sticker and affix it J A P A N Cover JAP AN UV 2A EX 330 380 DM 400 BA 420 Filter cube FL1...

Page 55: ...to the surface Securely attach the lamphouse cover to the lamphouse after replacing the lamp Never light the lamp with the lamphouse cover removed When you dispose of the replaced lamp do not break it up Instead dispose of the used lamp as special industrial waste or dispose of it according to the local regulations and rules 1 Attaching the lamphouse Before performing the following procedures turn...

Page 56: ...ocket Press the lamp as far as it goes and then release the lamp clamp lever to secure the lamp Be careful not to touch the glass surface with bare hands When releasing the lamp clamp lever use care so that the lamp does not tilt 5 Close the lamphouse cover and secure it by tightening the clamp screw 1 2 3 4 5 Attaching the Fiver Adapter and External Light Source If the brightness of the specified...

Page 57: ...sation filter comes with the HG fiber adapter Attach it into the bright field block in the LV UEPI2 The compensation filter is used to compensate the color balance and brightness If this filter is not used with extremely strong light will be radiated for the bright field microscopy Be sure to attach the filter 1 Check that the shutter of the illumination is closed and power supplies to the microsc...

Page 58: ...amp screw since the eyepiece tube may come off suddenly 7 Attaching Eyepieces Attach eyepieces of the same magnification and of the same viewfield number to the left and the right eyes There are positioning pins on the eyepiece sleeve Insert the eyepiece so that its positioning grooves match the pins 8 Attaching Objectives 1 Lower the stage completely 2 Screw objectives into the nosepiece so that ...

Page 59: ...ser 10 Attaching Column Riser The optional column riser is used for the adjustment of the distance between the objective and the stage when observing a thick specimen It is attached between the arm and the stage of the microscope When one column riser is attached the objective height rises 35 mm Attaching column riser 1 Remove the illuminator eyepiece and nosepiece if they are attached onto the mi...

Page 60: ...anufactured by EXFO connect the light source with the microscope through the RS 232C cable attached to the light source 13 Installing Separately Sold Accessories Install photomicrographic equipment and other separately sold accessories by referring to the system diagram or the instruction manual for each accessory 14 Anti static Treatment Many parts of the microscope have anti static finishes whic...

Page 61: ...o eliminate the influences of the vibration of the table The dimensions in the figure below are for brackets 5 mm thick Figure out the layout and dimensions of brackets on the table in a way that best fit your condition 4 Assembly 0 10 20 30 40 50 60 70 80 90 OBJ OFF RS232C including interference that may cause undesired operation This Class A digital apparatus complies with Canadian ICES 003 Cet ...

Page 62: ...s PC 1 Communication Method Asynchronous start stop synchronized serial communication RS 232C EIA standard compliant 2 Connector Specifications 1 Connector Type Name D sub 9 Pin Male 2 Pin Assignment Pin number 1 2 3 4 5 6 7 8 9 In out Input Output GND Signal name RxD TxD DTR SG DSR RTS CTS 1 6 2 3 4 5 7 8 9 Not used NOTE The control lines DTR DSR RTS and CTS are not used in communication with thi...

Page 63: ...mats The format of data received by this unit from an external device shall be defined as the receiving format and the format of data sent by this unit to an external device as the sending format Note that in the following text and are used as delimiters only for the purpose of description and that they are not part of the characters to be included in data sent or received 1 Receiving Format Ident...

Page 64: ...Acknowledging response against a c code Negative acknowledging response against a c or r code Acknowledging response against an r code Status transmission Error code a b d f 4 5 Specifications Indicates an unregistered command is received Indicates that data is invalid Indicates a timeout error occurred during control Indicates a control command is received while control is forbidden The received ...

Page 65: ...rotated by using the forward reverse rotation switch when the nosepiece is rotated by using the communication command or when the objective goes out of the optical path 2 The unit status will be sent when the the rotation driven by the motor ends or when the objective comes into the optical path 3 The shutter close command will be sent when the nosepiece is rotated by using the forward reverse rot...

Page 66: ...ata p 8 Response to Control Commands c RCW CR Rotates nosepiece in forward direction to the next address When properly finished o RCW CR When control error occurred n RCW error code CR c RCR CR Rotates nosepiece in reverse direction to the next address Rotation from address 1 to address 5 is prohibited When properly finished o RCR CR When control error occurred n RCR error code CR c RCC CR Rotates...

Page 67: ...bled or 2 external light source shutter control enabled r SAR CR Reads the status output setting the value set with cSAS command When properly finished a SAR data CR When control error occurred n SAR error code CR data 0 status output disabled 1 status output enabled or 2 external light source shutter control enabled s SAE data CR Outputs the status with the rotation of the nosepiece when the stat...

Page 68: ... p 49 Rotate to a clickstop position Mount it securely p 48 Cause Lamp not properly installed Optical path selection lever on the eyepiece tube is in an intermediate position Optical path selection lever on the eyepiece tube is not set to 100 or 20 binocular eyepiece Filter slider is in an intermediate position Field diaphragm is stopped down too far Nosepiece is not properly installed Nosepiece i...

Page 69: ...70 Use the specified objective p 56 Open to a suitable size p 32 Use a filter cube suitable for the specimen p 40 to 42 Attach cover glass It is not required for NCG objective however Use a nonfluorescent slide glass Cause Nosepiece is not properly installed Nosepiece is not rotated to a clickstop position Stage is slanted Microscope is not installed on a flat surface NCB11 filter is not used Lamp...

Page 70: ...p 27 Release the coarse focus stopper ring p 28 Tighten the torque adequately p 27 Release the coarse focus stopper ring p 28 Put it in the optical path p 36 and 38 Put it in the optical path p 39 Use objectives marked LU Plan or LU Plan Apo Match the prism selection according to the objective p 39 Put it in the optical path p 37 Cause Eyepiece diopter is not adjusted Eyepieces are not mounted cor...

Page 71: ...nect it securely P 53 Connect it securely p 49 Nosepiece cannot be rotated directly from address 1 to 5 Turn the nosepiece in the progressively ascending order of magnification Retry the rotation some times It can stop in the correct position after some retries p 35 Cause Power is not supplied Power cord is not connected to microscope Cable of lamphouse is not connected to the connector on microsc...

Page 72: ... eyepieces and glass plate according to the procedure described in 1 Cleaning Lenses and Filters without removing them from the microscope Nikon recommends cleaning them frequently Clean the bottom ends of objectives filters and the like to maintain the optical performance When cleaning the objectives remove them from the microscope Clean them whenever they are contaminated Microscopes and stages ...

Page 73: ...alcohol 2 Cleaning the Painted Plastic and Printed Parts Do not use organic solvents alcohol ether and paint thinner etc on painted plastic or printed parts Doing so could result in discoloration or in the peeling of printed characters If the dirt is hard to remove wipe it gently using a piece of gauze dampened with a neutral detergent solvent Use a piece of cleanroom wiper in the cleanroom instea...

Page 74: ... with coarse focus calibration marking for fine focus 1 µm marking Stroke 30 mm with coarse focus stopper mechanism Coarse focus knob 14 mm revolution Fine focus knob 0 1 mm revolution 10x field number 22 25 Input voltage 100 to 240 VAC 10 50 60 Hz Rated current 1 2 A max When the supply voltage is 100 V to 120 V UL Listed detachable cord set 3 conductor grounding Type SVT No 18 AWG 3 m long maxim...

Page 75: ... can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This class A digital apparatus complies with Canadian ICES 0...