Chapter 2 Microscopy
17
2.1 Diascopic Bright-field (BF) Microscopy (Continued)
3
Center the condenser.
3-1 Turn the field diaphragm knob until the dia
field diaphragm image becomes visible in the
field of view.
[l] Field diaphragm dial (
☞
3.3.3)
▼
3-2 Focus on the dia field diaphragm image.
[p] Condenser focus knob (
☞
3.4.2)
Adjust the focus so that the perimeter of the field
diaphragm can be clearly viewed.
If the lower limit of travel range is set, loosen the
refocusing clamp.
▼
3-3 Tighten the condenser refocusing clamp.
[t] Condenser refocusing clamp (
☞
3.4.2)
Tightening the condenser refocusing clamp restricts
the downward travel range.
▼
3-4 Move the dia field diaphragm image to the
center of the field of view and adjust the size
of the field diaphragm image to align the
image with the circumference of the field of
view.
[u] Condenser centering screw (
☞
3.4.3)
[l] Field diaphragm dial (
☞
3.3.3)
▼
3-5 For precise adjustment, change the objective
to a higher magnification objective and repeat
steps 3-1 to 3-4.
4
Change the objective and observe the
specimen.
4-1 Change the objective to the objective with a
specified magnification.
[b]
Nosepiece
(
☞
3.9.1)
▼
4-2 When using a liquid immersion type
objective, perform oil or water immersion.
[g]
Objective
(
☞
3.10.2 and 3.10.3)
▼
4-3 Remove one of the eyepieces, attach the
centering
telescope, and focus on the pupil of the
objective.
[r] Centering telescope (
☞
3.6.4)
▼
4-4 Adjust the size of the dia aperture diaphragm
to 70-80% of the size of the numerical aper-
ture of the objective.
[m] Aperture diaphragm open/close lever (
☞
3.4.5)
Adjust the size of the aperture diaphragm image while
observing the objective pupil plane and the aperture
diaphragm image using the centering telescope.
▼
4-5 Replace the centering telescope with the
removed eyepiece.
[r] Centering telescope (
☞
3.6.4)
▼
4-6 Adjust the size of the dia field diaphragm
image and move it to the center of the field of
view.
[l] Field diaphragm dial (
☞
3.3.3)
[u] Condenser centering screw (
☞
3.4.3)
▼
4-7 When using an objective with a correction
collar, adjust the correction collar.
[g]
Objective
(
☞
3.10.1)
Adjust the correction collar of the objective according
to the thickness of the prepared specimen slide or the
cover glass of the culture vessel.
▼
4-8 Move the stage and turn the focus knobs to
observe the sample.
[o] Stage handle (
☞
3.8.3)
[q] Focus knob (
☞
3.11.1)
