Chapter 2
STORM Microscopy Operation
13
5
Configure the N-STORM acquisition settings.
The settings are as follows.
Fluorescent probe checkbox:
Turn on the probe to be used.
Output of the activation laser (405 nm, 457 nm, 561
nm):
0.2% to 0.3% (values with which proper activation
is performed as shown in step 4 above)
Output of the imaging laser (647 nm):
100%
[Period Count] (number of periods for image
acquisition):
Any setting (normally 5000 to 20000 *
1
)
[Path], [File Name] (folder to which the file is to be
saved and the file name):
Any setting
[STORM Image] (whether to display the STORM
image during image acquisition):
If this checkbox is selected, the STORM image is
previewed during acquisition. Normally, this
should be selected.
[Graph] (whether to display a graph during image
acquisition):
If this checkbox is selected, a graph showing the
number of bright points in the image is displayed.
Normally, this should be selected.
[Minimum Height] (minimum intensity):
300 *
2
*1
The above number of periods for image acquisition
is a reference value. Adjust it according to the
structure or coloring status of the specimen. Even
when it is set to 20000 periods in advance, if it is
confirmed as being large enough on the real time
analysis (preview) screen, it is possible to halt the
acquisition (for example, about 10000 periods) by
clicking [Finish].
*2
The above-mentioned minimum height is a
reference value. Adjust it according to the amount of
background light for the specimen, etc.
6
Click [Run Now].
Disabling large image mode
If large image mode is enabled with the ND Acquisition
function of NIS-Elements, images cannot be acquired.
Disable the acquisition of large images before clicking
[Run Now].
5
6
