Ocean Optics QE Pro, Installation And Operation Manual

Page 1: ...QE Pro Scientific grade Spectrometer Installation and Operation Manual For Products QE Pro Document 891 00000 000 02 201603 ...

Page 2: ...n from Ocean Optics Inc Trademarks All products and services herein are the trademarks service marks registered trademarks or registered service marks of their respective owners Limit of Liability Every effort has been made to make this manual as complete and as accurate as possible but no warranty or fitness is implied The information provided is on an as is basis Ocean Optics Inc shall have neit...

Page 3: ...bout Ocean Optics Software 4 Sampling System Overview 4 How Sampling Works 4 Modular Sampling Accessories 4 Interface Options 4 Shipment Components 5 Other Accessories Available 6 Breakout Box 6 Chapter 2 Installing the QE Pro 7 Overview 7 QE Pro Installation 7 USB Mode 7 QE Pro Spectrometer Device Driver Installation 8 Installing the Driver Software for QE Pro Spectrometers on Windows 7 32 and 64...

Page 4: ...Calibrating the Wavelength of the QE Pro 22 Overview 22 About Wavelength Calibration 22 Calibrating the Spectrometer 23 Preparing for Calibration 23 Calibrating the Wavelength of the Spectrometer 23 Saving the New Calibration Coefficients USB Mode 25 Appendix B Specifications 27 Overview 27 How the QE Pro Works 27 QE Pro Components Table 28 QE Pro Specifications 29 CCD Detector Specifications 29 Q...

Page 5: ...ubleshooting Contains recommended steps to isolate and correct common problems Appendix A Calibrating the Wavelength of the QE Pro Provides instructions for calibrating the QE Pro Spectrometer Appendix B Specifications Contains technical specifications and connector pinouts for the QE Pro Spectrometer Product Related Documentation You can access documentation for Ocean Optics products by visiting ...

Page 6: ... com services exclusive 3 year warranty This comprehensive warranty ensures you of the highest level of craftsmanship and reliability for years to come No other manufacturer offers such a solid guarantee of quality and reliability The Ocean Optics 3 Year Warranty applies to Ocean Optics equipment excluding OEM configurations purchased on or after July 1 2010 The warranty covers parts and labor nee...

Page 7: ...rates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at his on expense WARNING The authority to operate this equipment is c...

Page 8: ......

Page 9: ...responsive from 200 1100 nm and has a peak quantum efficiency of 90 with a QE of 65 at 250 nm The detector s columns are binned or summed inside the detector prior to the readout process thereby minimizing readout noise Additionally the detector can be cooled from 40 C below ambient up to 50 C with the onboard TE Cooler to reduce dark noise The reduction of noise and dark signal allows integration...

Page 10: ...ometers INTERNAL SHUTTER LRG BENCH This makes it easier to take dark measurements and when you need fast integration times with the best possible signal This optional feature is also recommended for probe based or emissive measurements where it is difficult to introduce a shutter into the optical path The shutter can be added to an existing QE Pro for an extra charge Models The QE Pro is custom co...

Page 11: ...og timer for reliability Hardware power recycle via reset pin or software command Kensington security slot Optional shutter for dark measurements requiring a fast integration time and good throughput Specify when ordering LEDs for feedback on TEC readiness and general spectrometer health Software support OceanView OmniDriver SeaBreeze CE certification System Requirements You can use the QE Pro s U...

Page 12: ...s Consult the OceanView manual for hardware requirements when using OceanView see Product Related Documentation Sampling System Overview How Sampling Works Ocean Optics components function in a sampling system as follows in this example 1 The user stores reference and dark measurements to correct for instrument response variables 2 The light transmits through an optical fiber to the sample 3 The l...

Page 13: ...port Shipment Components QE Pro Spectrometer USB CBL 1 5VDC power supply The following information and documentation also ships with the QE Pro Spectrometer Packing List The packing list is inside a plastic bag attached to the outside of the shipment box the invoice arrives separately It lists all items in the order including customized components in the spectrometer such as the grating detector c...

Page 14: ...o offers the Breakout Box HR4 BREAKOUT a passive module that separates the signals from their 22 pin port to an array of standard connectors and headers enabling easy access to a variety of features found in Ocean Optics QE Pro Spectrometer In addition to the accessory connector the breakout box features a circuit board based on a neutral breadboard pattern that allows custom circuitry to be proto...

Page 15: ...serial modes USB Mode This section contains instructions for connecting the QE Pro in USB mode To connect the QE Pro to a computer via the USB port the computer must be running a Windows XP 7 8 10 or Vista 32 bit only Mac OS X 10 5 or later or Linux x86 or amd64 platform since 2010 operating system Procedure Follow the steps below to connect the QE Pro to a computer via the USB port 1 Install the ...

Page 16: ...he type of Windows system your computer is running Installing the Driver Software for QE Pro Spectrometers on Windows 7 32 and 64 bit When you first plug in your spectrometer Windows 7 will attempt unsuccessfully to automatically install the driver for your spectrometer Click close to close the warning message Procedure To manually install the new 64 bit driver for your spectrometer 1 Click Change...

Page 17: ...2 Installing the QE PRO 891 00000 000 02 201603 9 The system installs the device driver software When the installation process has finished successfully the following screen displays ...

Page 18: ...ollowing information Installing the Driver Software for QE Pro Spectrometers on on Windows XP 32 and 64 bit Procedure 1 When you first plug in your spectrometer Windows XP displays the following screen Choose No not this time 2 Choose Install from a list or specific location Advanced ...

Page 19: ...91 00000 000 02 201603 11 3 Navigate to C Program Files Ocean Optics OmniDriver winusb_drivers 4 The Progress window appears When the installation has completed the final window appears Click Finish to complete the installation ...

Page 20: ...Connector 3 Connect one end of the 9 pin serial cable to the adapter block on the QE Pro and then connect the other end to a serial port on the PC 4 Note the number of the serial port COM Port to which you connected the QE Pro some PCs may not have numbered ports handheld PCs typically have only one serial port LED Operation The QE Pro features two status LEDs LED Position Meaning Top LED Monitors...

Page 21: ... If you have followed the previous steps and started your spectrometer operating software the spectrometer is already acquiring data Even with no light in the spectrometer there should be a dynamic trace displayed in the bottom of the graph If you allow light into the spectrometer the graph trace should rise with increasing light intensity This means the software and hardware are correctly install...

Page 22: ...ro Acquisition Controls In OceanView the Acquisition Parameter Controls allow you to set the desired parameters for QE Pro TE Cooler Procedure To control the TE Cooler 1 Right click on the QE Pro in the Schematic View 2 Select New Acquisition Thermo Electric Cooling ...

Page 23: ... the Schematic View 2 Select New Acquisition Data Buffer Data buffering can also be set using the Acquisition Parameter Controls Procedure To add data buffering controls to the Acquisition Parameter Controls 1 Click on the Add Remove Controls tab 2 Select Data Buffer The Data Buffer controls appear on the Main Controls tab ...

Page 24: ...uffer to zero and start over Optional Shutter Procedure To enable the optional shutter 1 In the Acquisition Group window click on the Add Remove Controls tab 2 Select GPIO The GPIO controls appear on the Main Controls tab 3 Check the Out box for Index 4 This makes the GPIO an output 4 Check the Value box for Index 4 to close the shutter You can now take your dark measurement ...

Page 25: ...0 000 02 201603 17 5 Uncheck the Value box to open the shutter Notes Make sure the ALT checkbox for the GPIO Index 4 is not selected which would prevent the shutter from triggering The activation time of the internal shutter is 11 ms ...

Page 26: ... QE Pro Change the Slit There s no need to calibrate your spectrometer when changing the slit just install and start measuring There is one exception to this You cannot change from a standard slit to a slit with a filter because it changes the optical focus and wavelength calibration of the spectrometer In this case you would need to send the spectrometer back to Ocean Optics A filter must be orde...

Page 27: ...2 Installing the QE PRO 891 00000 000 02 201603 19 5 Install the 2 screws again Use the Allen key to tighten the screws carefully do not over tighten 6 If necessary connect the fiber again ...

Page 28: ...to remove the incorrectly installed device device driver and installation files Note If these procedures do not correct your device driver problem you must obtain the Correcting Device Driver Issues document from the Ocean Optics website http oceanoptics com wp content uploads Correcting Device Driver Issues pdf Remove the Unknown Device from Windows Device Manager Procedure 1 Open Windows Device ...

Page 29: ...m should now be able to locate and install the correct drivers for the USB device Apple Mac OSX Operating Systems Since there are no device files for the QE Pro Spectrometer in a Mac operating system you should not encounter any problems if you installed the spectrometer before the operating software Linux Operating Systems For Linux operating systems all you need to do is install the software the...

Page 30: ...htly as a function of time and environmental conditions Ocean Optics recommends periodically recalibrating the QE Pro About Wavelength Calibration You are going to be solving the following equation which shows that the relationship between pixel number and wavelength is a third order polynomial p I C1 p C2 p2 C3 p3 Where the wavelength of pixel p I the wavelength of pixel 0 C1 the first coefficien...

Page 31: ...brating the Wavelength of the Spectrometer Procedure Perform the steps below to calibrate the wavelength of the spectrometer 1 Place the spectrometer operating software into Quick View mode in OceanView and take a spectrum of your light source Adjust the integration time or the A D conversion frequency until there are several peaks on the screen that are not off scale 2 Move the cursor to one of t...

Page 32: ... 06 0 13 0 19 5 Use the spreadsheet or calculator to calculate the wavelength calibration coefficients In the spreadsheet program find the functions to perform linear regressions If using Quattro Pro look under Tools Advanced Math If using Excel look under Analysis ToolPak 6 Select the true wavelength as the dependent variable Y Select the pixel number pixel number squared and the pixel number cub...

Page 33: ...n the QE Pro You can overwrite old calibration coefficients on the EEPROM if you are using the QE Pro via the USB port Procedure To save wavelength calibration coefficients using the USB mode perform the following steps 1 Ensure that the QE Pro is connected to the PC and that you have closed all other applications 2 Point your browser to http oceanoptics com support software downloads and scroll d...

Page 34: ...enter the new values acquired in Step 5 of the Calibrating the Wavelength of the Spectrometer section in this appendix 7 Repeat Step 6 for all of the new values 8 Click on the Write Coefficients to Device button to save the information and then select File Exit to exit the QE Pro Reflash Utility software The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the ...

Page 35: ...ility It also includes accessory connector pinout diagrams and pin specific information How the QE Pro Works Below is a diagram of how light moves through the optical bench of a QE Pro Spectrometer The optical bench has no moving parts that can wear or break all the components are fixed in place at the time of manufacture QE Pro Spectrometer with Components ...

Page 36: ...o certain wavelength regions Only Ocean Optics technicians can change the Filter 4 Collimating Mirror Focuses light entering the optical bench towards the Grating of the spectrometer Light enters the spectrometer passes through the SMA Connector Slit and Filter and then reflects off the Collimating Mirror onto the Grating 5 Grating Diffracts light from the Collimating Mirror and directs the diffra...

Page 37: ... Pixel size 24 μm 2 Quantum efficiency 90 peak 65 at 250 nm See Quantum Efficiency of Detector graph below QE Pro Spectrometer Specifications Specification Value Integration time 8 ms to 60 minutes Typical dynamic range 85 000 1 Signal to noise ratio 1000 1 single acquisition Readout Noise 2 5 counts RMS typical Linearity Corrected 0 5 nonlinearity max f number f 4 Activation time of optional inte...

Page 38: ...age 4 5 5 5 V Power up Time 7 seconds Interfaces USB 2 0 480 Mbps USB 1 1 compatible RS 232 5 wire Dimensions LxWxH 182 mm 7 16 in x 110 mm 4 33 in x 47 mm 1 85 in Weight QE Pro Power Supply 1 15 kg 2 6 lbs 0 45 kg 1 lb System Compatibility You can use the QE Pro s USB connectivity with any computer that meets the requirements for the spectrometer operating software being used Windows XP Vista Win...

Page 39: ...on the front of the vertical wall of the QE Pro pin numbering is as follows USB Port 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 30 Pin Accessory Connector Pinout Diagram 30 Pin Accessory Connector Pin Definitions and Descriptions The following table contains information regarding the function of each pin in the QE Pro s 30 Pin Accessory Connector Pin Function ...

Page 40: ... Output Output power pin for QE Pro 13 SPI_MOSI Output SPI Master Out Slave In MOSI signal for communication to other SPI peripherals 14 VOUT Output Output power pin for QE Pro 15 SPI MISO Input SPI Master In Slave Out MISO signal for communication to the other SPI peripherals 16 GPIO 4 Input Output General Purpose Input Output 17 Single Strobe Output CMOS 3 3V output pulse used as a strobe signal...

Page 41: ...d to enable QE Pro transmission to the host 28 GPIO 8 Input Output General Purpose Input Output 29 Ground Input Output Ground 30 GPIO 9 Input Output General Purpose Input Output 30 Pin J2 Accessory Connector Part Numbers The part numbers for the 30 pin accessory connector on the QE Pro Spectrometer are as follows The connector is Pak50 model from 3M Corp Headed Connector Part Number P50 030P1 RR1 ...

Page 42: ...B Specifications 34 891 00000 000 02 201603 7 A_OUT 15 GPIO 7 8 ExtTrigIn ...

Page 43: ...e 24 CCD Detector 28 collimating mirror 28 compatibility 30 Components Table 28 configuring 13 in OceanView 13 configuring in OceanView buffering 15 TE Cooler 14 D Detector Collection Lens 28 document audience iv purpose iv summary iv E EEPROM 4 External Triggering 18 F features 2 filter 28 focusing mirror 28 G grating 28 I installation QE Pro spectrometer driver 8 QE Pro spectrometer driver on Wi...

Page 44: ...4 bit 10 QE PRO specifications 29 S Sampling Accessories 4 System 4 Serial Port mode 12 setup 7 shipment components 5 shutter control 16 slit 28 change 18 SMA Connector 28 specifications 27 QE PRO 29 spectrometer 29 spectrometer driver installation on QE Pro 8 spectroscopic accessories 17 System Requirements 3 T TE Cooler 1 14 30 Triggering 18 troubleshooting Linux systems 20 Mac systems 20 Troubl...