15
CKX41/CKX31
Aperture iris
diaphragm image
Fig. 18
Fig. 19
4-4 Illumination Column
1
Using the Filters
}Using appropriate filters according to the purposes allows you to observe
and photograph specimens more effectively. Particularly, the use of the LBD
filter is recommended in observation and photomicrography because it
renders more neutral colors.
}More than one filter can be stacked in the filter holder (Filter diameter: 45
mm. Maximum thickness of stacked filters: 11 mm).
Filter
Application
45IF550-W45
Monochrome contrast filter (Green)
45ND6, 45ND25
Light intensity adjustment filter
(Transmittance 6% and 25%)
45LBD2-N, 45KB
Color temperature conversion filter
(For observation and photomicrography)
45HA
(Heat absorbing filter)
Exposure time compensation in photomicrogra-
phy
2
Using the Aperture Iris Diaphragm
(Fig. 18)
}The aperture iris diaphragm determines the numerical aperture of the
illumination system in brightfield observation. It enables you to adjust the
depth of focus, contrast and resolution according to your requirements.
· Checking the aperture iris diaphragm:
Remove the eyepiece when necessary (and insert the CT-5 or U-
CT30 if you have one). Then look into the eyepiece sleeve; you
will seen the field of view as shown in Fig. 18. Now adjust the
aperture iris diaphragm lever as required.
· In general, when observing a dyed specimen, set the aperture iris dia-
phragm to 70% to 80% of the N.A. of the objective in use. However, when
observing a culture specimen, which is not dyed, set the aperture iris
diaphragm lever toward “ ”.
3
Removing the Condenser Lens
(Fig. 19)
}To provide more working distance, turn the condenser’s lower section
1
in the direction of the arrow and remove it. When you do this, however,
keep in mind that proper illumination cannot be achieved. Remove the
condenser lens only when using a large culture vessel.
@
Objective pupil
70-80%
30-20%
