APPLIED OPERATIONS
/Merger/Extraction of Image Channels
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Page
3. Display the image slice to start the range at the front using the <Display> button at
the top of the [Display] panel.
4. Click the <Set start position> button. (If the start position is not set, image No. 0
becomes the start image automatically.)
5.
Display the image slice to end the range at the front using the <Display> button.
6. Click the <Display> button. (If the end position is not set, image No. n-1, assuming
the number of image slices is n, becomes the start image automatically.)
2-13-2 Merging Image Channels
For instance, when a specimen is imaged with two scans for 2-channel fluorescence
observation and 1-channel transmitted light observation, the transmitted light image can
be merged to the fluorescence images to create a 3-channel image. Note that the
display gradation of the images obtained by overlaying 3 channels may be poor than
original.
1.
Open the two files to be used in creating a new image.
The image files used in overlay are subjected to the following
restrictions.
±
The sizes of the images in the two image files should be identical.
±
The number of data bits in the two image files should be identical.
(For example, it is not possible to overlay a Fluoview Multi Tiff file with
a Single TIF(S) 8-bit file.)
When the image in one of the image files is composed of multiple image
slices, it is possible to use only some of the slices by setting a slice range.
See section 2-13-1, “Setting the Range of Multiple Image Slices” for the
operation procedure.
When the images in both image files are composed of multiple image
slices, the overlaid image will have the same number of image slices as the
file with the fewer image slices.
Example 1: XYZ image + XY image = XY image
(The first image slice of the XYZ image is overlaid with the XY image.)
Example 2: XYZ image with 10 XYZ image with 5 slices = XYZ
image with 5 slices
(The first five image slices are overlaid.)
NOTE
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