TROUBLESHOOTING GUIDE
1-2
Page
Phenomenon
Cause
Treatment
Manual Ref. Pages
1. Fluorescence image
cannot be observed
The fluorescent dye and
excitation wavelength does
not match.
Select a laser suitable for the
fluorescent dye method.
Change the ND filters by
reading 1-2-7, “Selecting the
ND Filters” in OPERATION
INSTRUCTIONS.
1-2-7, OPERATION
INSTRUCTIONS
The fluorescent dye does not
match the excitation dichroic
mirror, dichroic mirror and/or
barrier filter.
Select the barrier filter
matching the fluorescent dye
method.
See 1-3-2-3, “Configuring
the Filters” in OPERATION
INSTRUCTIONS and follow
instructions of the [Optical
System Configuration]
window.
1-2-6 & 1-3-2-4,
OPERATION
INSTRUCTIONS
Focusing is not correct.
Adjust focusing.
1-2-3, OPERATION
INSTRUCTIONS
The photomultiplier tube
voltage of the detector is low.
Increase the photomultiplier
tube voltage using the [PMT]
scale in the [Acquire] panel.
2-2-1-4-9,
OPERATION
INSTRUCTIONS
The OFFSET value is too
high.
Decrease OFFSET to an
optimum value.
2-2-1-4-9,
OPERATION
INSTRUCTIONS
The photomultiplier tube of
the channel set with the
detection mode setting slider
is not set.
Check the [Ch1], [Ch2] ,
[Ch3] , [Ch4] or [Ch5] check
box in the [Acquire] panel.
1-2-8-3 & 2-2-1-4-3,
OPERATION
INSTRUCTIONS
2. Transmitted
image
cannot be observed.
The transmittance detection
channel is not selected.
In the [Acquire] panel, check
the check box of the [Ch]
with which “Transmitted” is
displayed.
2-2-3-3,
OPERATION
INSTRUCTIONS
The transmitted light filter is
engaged in the light path of
the microscope.
Disengage the filter from the
light path.
1-3-2-4,
OPERATION
INSTRUCTIONS
3. Image is disturbed.
The installation location is
subject to large vibrations.
Consult Olympus.
-
Darken the room before
acquiring image.
-
External light from a
fluorescent lamp, etc. is
detected.
Ensure that the top cover of
the scan unit is closed tightly.
-
4. Image is striped.
The laser reflection light is
penetrating.
The barrier filter selection is
erroneous.
1-2-6 & 1-3-2-4,
OPERATION
INSTRUCTIONS
5.
Image looks poor.
The analyzer is engaged in
the light path.
Disengage the analyzer from
the light path.
1-2-4, OPERATION
INSTRUCTIONS
The scan speed is high.
Decrease the scan speed to
an optimum speed or
integrate the image.
1-2-8-9 & 2-2-1-6,
OPERATION
INSTRUCTIONS
Summary of Contents for FLUOVIEW FV500
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