6
7
2
REFLECTED FLUORESCENCE OBSERVATION PROCEDURE
Preparation
Set the main switch to “ I ” (ON) and wait for the
lamp brightness to stabilize (5 to 10 minutes
after ignition).
Engage the fluorescence mirror unit matching
the specimen in the light path.
Engage the objective in the light path and focus
on the specimen
Adjust so that the
entire field is uniform
and brightest.
Adjust the brightness
as required.
Engage an ND filter in the
light path as required.
Place the specimen on the stage.
} If you need simultaneous observation of reflected fluorescence observation with the phase contrast observation or
transmitted light Nomarski Differential Interference Contrast (DIC) observation, please read Chapter 4, “SIMULTANEOUS
FLUORESCENCE OBSERVATION”. (Page 18)
(Controls Used)
(Page)
· Attach the fluorescence mirror unit and objective matching the observation method.
(Pages 9 to 10)
· Center the mercury burner.
(Pages 13 to 15)
Adjust the field iris diaphragm.
Adjust the aperture iris
diaphragm.
Observation
@Main switch
(P.11)
2
Slide holder
3
X-/Y-axis knobs
4
Mirror unit turret
5
Revolving nosepiece
6
Coarse/fine adjustment knobs
7
ND filter
(P.16)
8
Collector lens focusing knob
(P.13,14)
9
Field iris diaphragm knob
(P.11)
a
Aperture iris diaphragm knob
(P.12)
b
Shutter knob
(P.11)
BX3-URA
BX3-RFAS
} Engage the shutter if you interrupt observation for a short time.
