Olympus BX3-URA, Instructions Manual

Page 1: ...ize yourself fully with the use of this system we recommend that you study this manual thoroughly before operating the microscope Retain this instruction manual in an easily accessible place near the...

Page 2: ...complies with the requirements of both directive 2004 108 EC concerning electromagnetic compatibility and directive 2006 95 EC concerning low voltage The CE marking indicates compliance with the above...

Page 3: ...FAS BX3 URA 1 General Precautions for Observation 9 2 Selecting the Fluorescence Mirror Unit 9 3 Turning the Power Supply Unit On 11 4 Centering the Field Iris Diaphragm 11 5 Centering the Aperture Ir...

Page 4: ...using for replacement of the burner or any other internal part set the main switch to OFF then unplug the lamp housing connection cable from the power supply unit and wait for more than 10 minutes unt...

Page 5: ...flected fluorescence system Before using this system together with the BX3 microscope and associated options make sure that you have carefully read and understood their manuals and understand how the...

Page 6: ...s USH 103OL HBO103W 2 When the hour counter on the power supply unit indicates this value set the main switch to OFF and wait for more than 10 minutes before replacing the mercury burner Page 26 Unlik...

Page 7: ...ides Page 11 Shutter OPEN Shutter CLOSE Field iris diaphragm knob Page 11 Analyzer insertion slot Page 18 Field iris diaphragm centering screws Page 11 x2 screws Collector lens focusing knob Page 13 1...

Page 8: ...hutter OPEN Shutter CLOSE ND filter slider insertion slot Page 16 Insertion from right side Aperture iris diaphragm knob Page 12 Field iris diaphragm knob Page 11 Field iris diaphragm centering screws...

Page 9: ...ndicator sheets provided with the illuminator can be used to write the names of original fluorescence mirror units Power Supply Unit U RFL T U RX T ND Filters BX3 25ND6 BX3 25ND25 Centering Target U C...

Page 10: ...the phase contrast observation or transmitted light Nomarski Differential Interference Contrast DIC observation please read Chapter 4 SIMULTANEOUS FLUORESCENCE OBSERVATION Page 18 Controls Used Page...

Page 11: ...8 Make a photocopy of the observation procedure pages and post it near your microscope 9 a 7 5 2 8 3 6 b 1 4...

Page 12: ...of specimens This system features high excitation light intensity to ensure bright observation of dark fluorescence specimens In consequence after long period of observations using high power objecti...

Page 13: ...530 550 BA575IF Rhodamine TRITC Florescent antibody Propidium iodide DNA RFP Y U FYW DM600 BP545 580 BA610IF Texas Red Fluorescent antibody Combinations for color separation U U FUNA DM410 BP360 370 B...

Page 14: ...after use After replacing the mercury burner reset the hour counter by holding its reset button till 0 0 is displayed CAUTION CAUTION Fig 1 4 Centering the Field Iris Diaphragm Fig 1 1 Close the light...

Page 15: ...center of field 6 Turn the revolving nosepiece to engage the empty place the objective cap should be removed in the light path 7 Pull out the aperture iris diaphragm knob 2 to minimize the aperture i...

Page 16: ...n the cross lines for reflected light white surface 5 While looking into the eyepieces adjust the stage to bring the center of the cross lines for reflected light on the white surface on the center of...

Page 17: ...he shutter by setting the shutter knob to position marked 8 While looking into the eyepieces turn the collector lens focusing knob to project the arc image and mirror arc image on the U CST A in Fig 7...

Page 18: ...t has been executed the mirror can never be returned to the same status as the factory shipment status 1 Using a pair of tweezers etc peel off the two blind seals 6 from the rear of the lamp housing 2...

Page 19: ...ed back to front with the filter name inscription facing the opposite direction to the observer the filter may be damaged The first click position of the filter engages the empty position in the light...

Page 20: ...ser and the area around it In this case use the light shield sheet 1 Lower the condenser by turning the condenser knob 2 Insert the light shield sheet into the space on the stage When switching the ob...

Page 21: ...l provided with the U UCD8 transmitted light universal condenser The transmitted light DIC observation requires the following accessories universal condenser U UCD8 2 transmitted light DIC slider U DI...

Page 22: ...t correctly engaged in light path Make sure that revolving nosepiece clicks properly into place Fluorescence mirror unit is not correctly engaged in light path Engage fluorescence mirror unit correctl...

Page 23: ...2 U excitation Narrow band U FUN Transmittance Wavelength nm 6 B excitation Narrow band U FBN Transmittance Wavelength nm 3 V excitation Narrow band U FVN Transmittance Wavelength nm 7 G excitation Wi...

Page 24: ...on Wide band U FBWA Transmittance Wavelength nm 14 For cyan fluorescent protein CFP U FCFP Wavelength nm Transmittance 11 IB excitation color separation Narrow band U FBNA Transmittance Wavelength nm...

Page 25: ...velengths of 365 366 404 7 435 546 1 and 577 0 579 1 nm In addition light beams having wavelengths of 334 2 and 490 nm at rather low luminance are also applicable to fluorochrome emission Typical Exam...

Page 26: ...t control box For acquisition of mirror unit position information Lamp housing for mercury burner Lamp housing for 100 W mercury burner U LH100HG Apo lamp housing for 100 W mercury U LH100HGAPO Mercur...

Page 27: ...or clamping the screws inside the illuminator to retain the performance have your Olympus representative conduct this work Be sure to insert the sliders in the orientations shown in the diagram Otherw...

Page 28: ...Otherwise the fluorescence mirror unit may be mounted being tilted Two more fluorescence mirror units can be mounted in the turret positions on the left and right in the current condition Write down...

Page 29: ...burner to the original position and tighten the clamping screw Align the external edges of the lamp housing with those on the socket section and push the lamp housing straight down ward Attach the la...

Page 30: ...ly Place each filter by aligning the directions of the arrows located on the side surfaces toward the absorption filter frame and the main body respectively Fit the holder ring driver into the groove...

Page 31: ...inspections are charged after expiration of the warranty period If you have any questions please contact Olympus Check results Date Check items 1 More than 8 years have passed since original purchase...

Page 32: ...ng Table 1 Certified Cord A power supply cord should be certified by one of the agencies listed in Table 1 or comprised of cordage marked with an agency marking per Table 1 or marked per Table 2 The f...

Page 33: ...Cables BASEC BASEC HAR 10 10 30 N V KEMA KEMA KEUR HAR 10 30 30 SEMKO AB Svenska Elektriska Materielkontrollanstalter SEMKO HAR 10 10 50 sterreichischer Verband f r Elektrotechnik VE VE HAR 30 10 50...

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