Olympus BX51, Instructions Manual

Page 1: ...btain optimum performance and to familiarize yourself fully with the use of your microscope we recommend that you read this manual together with the instruction manual for the BX51 BX52 microscope thoroughly before use Retain this instruction manual in an easily accessible place near the work desk for future reference A X 6 3 0 7 ...

Page 2: ...Detailed Assembly Procedure 4 9 3 CONTROLS 10 11 4 USING THE CONTROLS 12 15 4 1 Stage 12 14 4 2 Intermediate Polarizing Attachment 15 5 POLARIZED LIGHT OBSERVATION 16 26 5 1 Adjustments Before Observation 16 24 5 2 Orthoscopic Observation 25 5 3 Conoscopic Observation 26 6 SPECIFICATIONS 27 28 7 OPTICAL CHARACTERISTICS 29 8 TROUBLESHOOTING GUIDE 30 ...

Page 3: ... microscope where it is subjected to direct sunlight high temperature and humidity dust or vibrations Install it on stable level bench For operation environment conditions see SPECIFICATIONS on page 28 If the microscope is used in a manner not specified by this manual the safety of the user may be imperiled In addition the equipment may also be damaged Always use the equipment as outlined in this ...

Page 4: ...ing intermediate attachment Centerable revolving eyepiece Polarizing rotatable stage Strain free condenser Observation tube BX51 52 Microscope frame Objective Strain free objective series Lamp housing Components for polarized light observation ...

Page 5: ...2 1 Assembly Diagram Eyepiece Centerable revolving nosepiece U P4RE Test plate adapter U TAD Sensitive tint plate U TP530 Quarter wave plate U TP137 Strain free objective Mechanical stage U FMP Stage clips Polarizing rotatable stage U SRP Polarizing intermediate attachment U CPA Observation tube Intermediate attachment for AN360P U OPA Rotatable analyzer U AN360P 2 Allen screwdriver Lamp housing H...

Page 6: ...ge bracket with the positioning pin on the stage aligned with the groove on the front of the stage bracket then loosely tighten the centering knobs Mounting Stage Clips and Mechanical Stage U FMP Fig 2 Insert the stage clips securely into any two holes ƒ on the stage top surface Mount the mechanical stage so that the positioning pins on the under side fit into the positioning holes on the stage to...

Page 7: ...crew 4 Position the condenser with the scale markings in front insert it into the substage fork as far as it will go Align the positioning pin at the back of the condenser with the groove in the substage fork Swing the top lens out of the way before inserting the condenser 5 Tighten the condenser clamping screw then raise the condenser to its upper limit 2 Mounting the Condenser U POC 2 Fig 3 Fig ...

Page 8: ... a clock wise direction from the primary hole 4 Mounting the Revolving Nosepiece U P4RE Fig 5 1 Turn the coarse adjustment knob to lower the stage all the way 2 Using the Allen screwdriver loosen the nosepiece clamping screw on the microscope frame 3 Carefully slide the nosepiece along the dovetail to its limit in the direction of the arrow 4 Clamp the nosepiece by tightening the nosepiece clampin...

Page 9: ...ate tube can be adjusted later At this point place the tube so the nameplate is toward the rear 1 Using the Allen screwdriver loosen the observation tube clamping screw on the microscope frame 2 Insert the circular dovetail mount at the bottom of the intermediate attachments into the opening on the microscope frame and clamp by tightening the clamping screw Fig 6 5 Mounting the Intermediate Attach...

Page 10: ... the observation tube by tightening the clamping screw 7 Mounting the Eyepieces Insert the eyepiece with cross lines into the right hand eyepiece sleeve Make sure the eyepiece is inserted so that the positioning pin on the eyepiece is inserted into the groove at the lower end of the sleeve 1 Loosen the revolving nosepiece clamping knob and remove the dummy slider 2 Insert the test plate adapter an...

Page 11: ...the test plate adapter U TAD Position the fixed analyzer so that the positioning pin on the fixed analyzer fits into the groove The fixed analyzer will be held in place by a magnet 1 Loosen the test plate adapter U TAD clamping knob 2 Insert the sensitive tint plate U TP530 quarter wave plate U TP137 or any of the other compensators 6 types into the test plate adapter and securely tighten the clam...

Page 12: ...o duced on the following pages Bertrand lens knob CONTROLS Slider clamping knob Mechanical stage Vernier scale Aperture iris diaphragm lever Polarizer rotation ring Bertrand lens focusing knob Analyzer Slot for compensators Stage rotation clamping knob Polarizer clamping knob ...

Page 13: ...Rotatable Analyzer U AN360P 2 Empty hole ND filter insertion possible Stopper knob Rotation dial Clamping knob Test Plate Adapter U TAD Test plate clamping knob Hole for the fixed analyzer U ANT Slot for compensator plate Sensitive Tint Plate U TP530 Quarter Wave Plate U TP137 ...

Page 14: ...nter and clamp the specimen with the stage clips When Using the Mechanical Stage U FMP Fig 12 Open the spring loaded finger and place the specimen on the stage Applicable slide glass is for inorganic substances 28 x 48 mm and organic substances 26 x 76 mm with cover glass thickness of 0 17 mm Inorganic specimen Organic specimen Fig 11 ...

Page 15: ...cated at the right side of the stage is moved toward the observer and the stage is moved from this position to the first click stop position the specimen is moved 45 to its diagonal position To release the 45 click stop function push back the lever When releasing the 45 click stop function by pushing back the lever do so at a click stop position Fig 13 2 Stage Rotation Fig 13 ...

Page 16: ...e 4 2 Using the Allen screwdriver loosen the substage bracket clamping screw and remove the substage assembly Fig 15 3 Turn the coarse adjustment knob and raise the focusing block to where the stopper screw in the arm becomes visible Fig 16 4 Using the Allen screwdriver loosen and remove the upper stopper screw 5 Reattach substage assembly and stage Store the remove stopper screw in a safe place s...

Page 17: ...zer Fig 18 The analyzer is engaged when pushed in to the second click stop position To disengaged the analyzer and to place the empty hole in the light path pull the analyzer out to the first click stop 3 Focusing the Conoscopic Image U CPA only Fig 18 To focus the conoscopic image manipulate the Bertrand lens focusing slider While observing the conoscopic image move the slider to find the positio...

Page 18: ...sure the 10X objective is inserted into the primary hole on the centerable revolving nosepiece 1 Fully insert the alignment polarizer provided with the U OPA into the test plate adapter U TAD Tighten its clamping knob Fig 19 2 Remove the condenser 3 Insert the rotatable analyzer U AN360P 2 into the analyzer slot on the intermediate attachment Engage the analyzer loosen the clamping screw and set t...

Page 19: ... the specimen 2 Focus on the specimen and look for an easily recognizable detail in the field Move this detail in the center of the eyepiece cross lines 3 When the stage is rotated the detail moves in circle Manipulate the two stage centering knobs to coincide the imaginary center of the circle circumscribed by the detail with the intersection of the eyepiece cross lines 4 Moving the specimen only...

Page 20: ...ll out the Bertrand lens knob to the OUT position Fig 23 2 Center the condenser Refer to page 18 in the BX51 52 instruction manual 3 Follow steps 9 and 10 in When Using the Intermediate Attachment for AN360 Fig 22 10 This concludes centering of the optical axis for the 10X objective which will act as the reference objective Now center the other objectives with the centerable nosepiece by engaging ...

Page 21: ...tomicrography described in the following paragraph When Using Polarizing Intermediate Attachment U CPA Photomicrography 1 Using the tip of a mechanical pencial etc push out in the direction of the objective hole the black rubber plugs inserted into the centering holes of the primary hole where the 20X or 10X objective is inserted 2 Pull out the Bertrand lens knob to the OUT position Fig 25 3 Adjus...

Page 22: ... 7 Insert the two provided centering wrenches into the centering holes for the primary hole with the 20X or 10X objective on the nosepiece 8 Manipulate the centering wrenches to bring the bright center portion of the conoscopic image into the center of the field of view Fig 27 9 Pull out the Bertrand lens knob to the OUT position At this state center the condenser again as in step 4 above Fig 26 F...

Page 23: ...ecentration this will move the specimen detail in the opposite direc tion away from the cross line center 4 Moving the specimen only move a new specimen detail into the cross line center Repeat steps 3 and 4 several times until the center of the stage rotation is in the center of the cross lines i e when rotating the stage the specimen detail stays in the center of the cross lines 5 Clamp the stag...

Page 24: ...atable analyzer into the light path and set vibration direction scale at the 0 position Clamp with the clamping knob Fig 30 3 Set the polarizer scale at the 0 position 4 Loosen the polarizer clamping knob Rotate the polarizer rotation dial until complete extinction is obtained At this point tighten the clamping knob Fig 30 Fig 30 2 Adjusting for Extinction Fig 30 ...

Page 25: ...ientation plate B2 PJ Remove the test plate adapter and the compensator from the light path 1 Swing out the top lens of the condenser 2 Place the orientation plate B2 PJ on the stage Remove the analyzer from the light path and use the 4X objective to focus Fig 31 3 Adjusting the Eyepiece Cross Lines Fig 31 ...

Page 26: ...ving rotate the stage to locate the position where the orientation plate is darkest At this point clamp the stage 5 Remove the analyzer from the light path Set to brightfield and slightly loosen the observation tube clamping screw 6 Rotate the observation tube to position the eyepiece cross line parallel to the fiducial line of the orientation plate Tighten the observation tube clamping screw Orie...

Page 27: ...and lens from the light path 2 Insert the analyzer and perform observation When the top lens is swung out the aperture iris diaphragm and field iris diaphragm will not function normally If the aperture iris diaphragm is stopped down the field of view may be restricted 3 Insert a test plate sensitive tint plate U TP530 quarter wave plate U TP137 into the test plate slot When fully inserted the plat...

Page 28: ...6 Slide the focusing knob on the U CPA to focus on the conoscopic image When the polarizing intermediate attachment U CPA is not used conoscopic observation is possible by removing an eyepiece from the observation tube and looking at the rear focal plane of the objective directly To obtain good image contrast place an interference filter 45IF546 in the filter holder on the light exit of the micros...

Page 29: ...lens knob position for switch between orthoscopic and conoscopic observation Pushed in position IN Pulled out position OUT 2 Analyzer U AN360P 2 Analyzer slot Slot for rotatable analyzer U AN360P 2 3 Revolving nosepiece U P4RE 360 dial rotation Minimum scale reading 0 1 Vernier scale Type Centerable quadruple Attachable compensators Quarter wave plate U TP137 sensitive tint plate U TP530 and all k...

Page 30: ... Co manufacture attachable 5 Condenser U POC 2 Aplanatic achromatic condenser top lens swing out type Polarizer 360 rotatable 0 position adjustable N A 0 9 top lens swung in Applicable objectives 2X to 100X 2X to 4X objectives are used with the top lens swung out Operating environment Indoor use Altitude up to 2000 m Temperature 5 to 40 C 41 to 104 F Maximum relative humidity 80 for temperatures u...

Page 31: ...on Mechanical tube length Designation Numerical aperture N A Cover glass thickness Optical character Magni fication N A W D mm Cover glass thick ness Reso lution µm Eyepiece Remark Objective WH10X FN22 WH15X FN14 Total mag Depth of focus µm Field of view Total mag Depth of focus µm Field of view Plan P Strain free Plan Apochromat FN22 Ach P Strain free Achromat FN22 UPlan Fl P Strain free Universa...

Page 32: ... from the light path b Vignetting or uneven field of view Test plate is stopped at an intermediate position Set at click stop position During orthoscopic observation the con denser top lens is placed in the light path or stopped at intermediate position Remove top lens completely from the light path c The conoscopic image is not visible Condenser top lens is not placed in the light path Swing in t...

Page 33: ...MEMO ...

Page 34: ...er Drive Melville NY 11747 3157 U S A 491B River Valley Road 12 01 04 Valley Point Office Tower Singapore 248373 2 8 Honduras Street London EC1Y OTX United Kingdom 104 Ferntree Gully Road Oakleigh Victoria 3166 Australia This publication is printed on recycled paper Printed in Japan 2001 09 M 005 ...