Olympus CX43, Instructions Manual

Page 1: ...fully with the use of this microscope we recommend that you study this manual thoroughly before operating this microscope and always keep this manual at hand when operating this product Retain this instruction manual in an easily accessible place near the work desk for future reference Biological Microscope CX43 A X 8878 Optical Microscope and Accessory ...

Page 2: ...on against harmful interference when the product is operated in a commercial environment This product generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this product in a residential area is likely to cause harmful interference in which case the user will b...

Page 3: ...ortion 14 1 Selecting the objective 14 4 4 Focusing portion 14 1 Focusing on the specimen 14 2 Adjusting the tension of the coarse focusing knob 15 3 Using the pre focusing lever 15 4 5 Tube portion 16 1 Adjusting the interpupillary distance 16 2 Using the eye shades 16 3 Adjusting the diopter 17 4 Trinocular tube U CTR30 2 18 5 Tilting binocular tube U CTBI 18 6 Intermediate attachment 18 4 6 Con...

Page 4: ...ting 32 9 Specifications 34 10 List of optical performances 35 11 Assembly 37 11 1 Assembly diagram 37 11 2 Assembly procedures 38 1 Attaching the intermediate attachment and the tube 38 2 Attaching the camera adapter 38 3 Attaching the eyepieces WHB10X WHB10X H 39 4 Attaching the eyepiece micrometer 39 5 Attaching the objective 40 6 Attaching the specimen hold plate CX3 SHP or the specimen holder...

Page 5: ...mens from touching your skin directly When you maintain the product which may have contacted the specimens which have potentiality of infection wear protective equipment such as gloves etc or clean the product before operation After observation clean the portions where specimens contacted directly Remove the specimen when moving the product When moving this product be sure to remove the specimen f...

Page 6: ...not be assured Do not use this product near the sources of strong electromagnetic radiation Proper operation may be interfered Be sure to evaluate the electromagnetic environment before operating the product Disconnect the power cord in case of emergency In case of emergency disconnect the power cord from the power cord connector of the product or from the power outlet Install the product at the l...

Page 7: ...ope disconnect the AC adapter from the microscope and store the AC adapter and the power cord in the microscope frame Then hold both sides of the arm as shown in the lower left picture and carry it carefully Then hold both sides of the arm a as shown in the picture on the left and carry it carefully For procedures to store the AC adapter and the power cord see Storing the AC adapter and power cord...

Page 8: ...dust over made by Olympus contact Olympus Do not use a highly sealable cover such as a plastic bag etc as a dust cover The humidity in the microscope may increase to damage the product 4 When disposing of this product be sure to follow the regulations and rules of your local government Contact Olympus for any questions CAUTION NOTE NOTE Intended use This product has been designed to be used to obs...

Page 9: ...are configuration units of the CX43 system including options For other combinable units refer to the latest catalogs or contact Olympus TIP Tube Eyepieces Revolving nosepiece Stage Specimen holder Power cord AC adapter Objective Microscope frame Intermediate attachment Condenser ...

Page 10: ...t ring Binocular portion Main switch X axis knob Fine focusing knob Y axis knob Y axis knob Fine focusing knob Brightness adjustment knob Various filters Pre focusing lever Condenser top lens Ring slit centering knob Coarse focusing knob Ring slit centering knob Ring slit centering knob Aperture diaphragm Aperture diaphragm lever Tension adjustment ring Tension adjustment ring Tension adjustment r...

Page 11: ... bring the specimen into focus Bring the specimen into focus and adjust the brightness Set the main switch to ON Place the necessary filter Adjust the brightness Select BF brightfield observation This section describes the procedures of the transmitted brightfield darkfield observation which is the basic of all observations The transmitted phase contrast observation and transmitted simple polariza...

Page 12: ...8 Make a copy of this observation procedure guide and put it near the microscope to use for observation TIP 1 2 3 4 5 6 9 7 b a 8 ...

Page 13: ...ortion 4Operating procedures of each portion 2 Using the filter Engage the filter in the light path if necessary Engage the filter in the filter holder window lens a of the base portion of the microscope frame One to several filters of 45mm in diameter can be inserted in stack Insertable thickness up to 1 6mm If it is difficult to attach the filter due to the narrow space rotate the coarse focusin...

Page 14: ...the specimen as shown in the picture on the left and slide the specimen from front to back on the stage to place it 1 2 3 NOTE 1 2 3 b For procedures to attach the specimen holder option see page 41 When using the specimen hold plate CX3 SHP Option Rotate the coarse focusing knob a in the arrow direction to fully lower the stage Place the specimen on the resin sheet b The outside of the aperture c...

Page 15: ...to adjust it When attaching the stage knob rubber insert the larger one to the Y axis knob upper side and the smaller one to the X axis knob lower side from below 1 2 NOTE TIP a c b 2 Moving the specimen When using thethe specimen holder CX3 HLDT Option Rotate the coarse focusing knob a in the arrow direction to fully lower the stage Press the specimen holding lever knob b backward arrow direction...

Page 16: ...de scale vernier scale b The scale indicates the unit of 0 1 mm The position shown in the left picture can be read as 137 8 mm 1 2 TIP Fixing the stage If you want to move the observation position by moving the specimen with your finger without using the specimen holder the stage can be fixed so that it does not move unexpectedly Move the X axis Y axis knobs to match the hole a at the back right o...

Page 17: ... image How to track an observation image The up down and left right movements of the image observed through eyepieces are opposite to the movements of the specimen NOTE Slide glass It is recommended to use the following slide glasses a Length 76 mm Width 26 mm Thickness 0 9 to 1 4 mm Thickness 0 9 to 1 2 mm for darkfield observation Use the cover glass b with the thickness of 0 17mm to fully deliv...

Page 18: ... rotate the fine focusing knob b to bring the specimen into focus precisely 1 2 3 4 4 Focusing portion 4 3 Revolving nosepiece portion 1 Selecting the objective Hold the revolving nosepiece a and rotate it so that the intended objective comes exactly above the specimen Do not rotate the revolving nosepiece by holding the objective Be careful if you rotate the revolving nosepiece while observing th...

Page 19: ...er position again It is recommended to use the pre focusing lever to prevent the specimen from damage but if you do not use the pre focusing lever rotate the pre focusing lever in the direction opposite to the arrow direction until it stops NOTE TIP The tension of the coarse focusing knob can be adjusted Insert a commercially available flathead screwdriver into the groove c of the tension adjustme...

Page 20: ...e right and left fields of view coincide completely The value shown by the indicator c on the eyepiece sleeve represents your interpupillary distance Be sure to make the interpupillary distance same between right and left Note your interpupillary distance so that it can be easily adjusted next time 1 NOTE TIP When wearing eyeglasses Use the eye shades in the normal folded down position When not we...

Page 21: ...nto focus Change to the 40X objective again and rotate the coarse fine focusing knobs to bring the specimen into focus Change to the 10X objective and check that the specimen is in focus by looking through the right and left eyepieces If the specimen is not in focus repeat from 7 to 9 1 2 3 4 5 6 7 8 9 TIP a b 0 10 20 30 40 50 60 70 80 90 100 Eyepiece micrometer For attaching the eyepiece micromet...

Page 22: ...Trinocular tube U CTR30 2 Combining the trinocular tube allows you to acquire the image of the specimen using the camera The ratio of the observation light intensity is fixed as Eyepiece side 50 Camera side 50 For attaching the trinocular tube to the microscope frame and attaching the camera adapter and the camera see page 38 Intermediate magnification changer U CA U ECA U ECA1 6X Eye point adjust...

Page 23: ...se contrast observation see page 23 Ph2 Ph3 FL Fluorescence observation 2X Brightfield observation Select when using 2X objective Fixing the turret If no observation method other than the brightfield observation is selected the turret can be fixed so that it does not move from the BF position unexpectedly The turret can be fixed with the stage fixing knob see page 12 Both the stage and turret cann...

Page 24: ...cimen with the best contrast and resolution For the aperture diaphragm see page 31 Move the aperture diaphragm lever a to right and left direction to match with the numerical aperture NA of the objective in use For darkfield observation fully open the aperture diaphragm If you want to fix the aperture diaphragm at the desired position rotate the aperture diaphragm fixing knob b at the back of the ...

Page 25: ...tion with the 100X oil immersion objective If you do not apply the immersion oil the image observed with the 100X oil immersion objective becomes slightly dark For observation with 4X to 40X objectives it is not necessary to apply the immersion oil between the tip of condenser lens and the specimen The brightness of the observed image is same regardless of whether the immersion oil is applied or n...

Page 26: ...il without wiping it off the oil may be adhered to cause the incorrect observation The oil pan b can temporarily store the immersion oil flowed down from the condenser lens Clean the oil pan regularly to prevent the immersion oil from overflow If the immersion oil is overflowed from the oil pan b wipe off the immersion oil with the cleaning paper or the gauze slightly moistened with absolute alcoh...

Page 27: ...phase contrast in the light path Center the ring slit 6 Coarse fine focusing knobs 14 Observation b Various filters 9 7 Brightness adjustment knob 9 Adjust the brightness Place the necessary filter Select either Ph1 Ph2 or Ph3 phase contrast observation a Ring slit centering knob 25 Operation portion Page 2 Condenser turret 19 5 Revolving nosepiece 14 1 Main switch 9 3 Specimen holder 10 4 X axis ...

Page 28: ...24 1 2 3 4 5 6 9 7 a a 8 b ...

Page 29: ...ng slit bright ring in the field of view and the phase plate dark ring of the objective cannot be viewed clearly remove the specimen from the stage Push the centering knob 2 positions into the condenser turret toward inside front side Rotate the centering knob 2 positions keeping them pushed in to adjust so that the ring slit bright ring c overlaps the phase plate dark ring d In a same manner perf...

Page 30: ... attaching procedures see 11 Assembly on page 37 Select BF brightfield observation 4 Revolving nosepiece 14 Engage the objective for polarization you use in the light path a Coarse fine focusing knobs 14 Bring the specimen into focus and adjust the brightness c Simple polarizing intermediate attachment 28 Engage the analyzer for simple polarization in the light path Place the polarizer Adjust the ...

Page 31: ...27 CX43 2 3 4 5 6 8 9 b a c d 7 1 ...

Page 32: ...t touches the end and tighten the fixing knob b According to procedures on page 41 attach the polarizer for simple polarization c to the filter holder window lens of the base portion of the microscope frame Engage the 10X objective in the light path While looking through the eyepieces rotate the polarizer for simple polarization c with your hand to set to the status where the field of view becomes...

Page 33: ...s determined by the size of the image sensor used in the camera and the magnification of the camera adapter The image acquisition ranges for the observation range of the eyepiece WHB10X Field number 20 are as follows Digital camera for microscope DP22 Picture A on the left Image sensor size diagonal 1 1 8 inch 9mm The image acquisition range a Camera adapter 1x U TV1XC 9mm b Camera adapter 0 63x U...

Page 34: ...is to just magnify an image already resolved by the objective The field number is the diameter of the image viewed through eyepieces indicated in millimeters Even with the same magnification the larger the field number is the larger field of view can be observed at a time Standard 10X eyepiece 20mm WHSZ15 x H 16mm Actual field of view Field number Actual field of view Magnification of objective Ex...

Page 35: ...of each objective allows you to observe the specimen with the best contrast and resolution Numerical aperture of objective and numerical aperture of condenser suitable for observation In general since the contrast of the specimen to be observed with microscope is low it is appropriate to adjust the numerical aperture of the condenser to approximately 70 to 80 of the numerical aperture of the objec...

Page 36: ...clear The objective is not engaged in the light path properly Rotate the revolving nosepiece until it clicks to engage the objective in the light path The objective eyepiece condenser window lens or and specimen are dirty Clean them thoroughly 4 The immersion oil is not used with an oil immersion objective Use the immersion oil 21 The immersion oil contains bubbles Remove the air bubbles 21 The sp...

Page 37: ...d correctly Adjust it correctly 16 The difference in diopter of two eyes is not corrected properly Correct it properly 17 Different eyepieces are used for right side and left side Use the same eyepiece for right side and left side 39 13 When changing the objective from lower magnification to higher magnification the objective collides with the specimen The specimen is placed upside down Place the ...

Page 38: ...pecimen holder CX43LF CX43RF Possible to hold 2 slide glasses Possible to hold 1 slide glass Option CX3 SHP Specimen hold plate CX3 SHP Specimen hold plate CX3 HLDT Specimen holder Condenser Model type Abbe condenser Numerical aperture 1 25 when immersed with oil Aperture diaphragm Illumination aperture diaphragm blade manual open close type Turret selection type BF DF FL Ph1 Ph2 Ph3 2X Dimension ...

Page 39: ...0XP ACHN40XP ACHN100XOP Plan Achromat for phase contrast PLCN10XPH PLCN20XPH PLCM40XPH PLN 100XOPH 1 Not available for darkfield observation 10List of optical performances The following table shows the optical performances when combining eyepieces and objectives The picture on the right shows the various performances indicated on the objectives Magnification Mechanical tube length Objective type o...

Page 40: ...ical aperture corresponds to F number of the camera and is related to the resolution The resolution becomes higher when the numerical aperture becomes larger F number is a value obtained by dividing the focal distance of lens by the valid aperture diameter This is used as an index to show the brightness of the lens Working distance W D Distance between the top surface of the cover glass and the ti...

Page 41: ... DP27 Eyepiece micrometer Trinocular tube U CTR30 2 Binocular tube etc U CBI30 2 Tilting binocular tube etc U CTBI Polarizer U POT Diameter 45mm Filter UIS objective Outside diameter 24mm Simple polarizing intermediate attachment CX3 KPA Eye point adjuster U EPA2 Opposed discussion tube U DO3 Drawing device U DA Arrow pointer U APT Trinocular intermediate attachment U TRU U TRUS Intermediate magni...

Page 42: ...amping screw b of the intermediate attachment to secure the tube firmly When not attaching the intermediate attachment After 1 fit the circular dovetail of the tube into the tube attaching portion of the microscope frame and face the scale values of the interpupillary distance of the tube to the front Tighten the tube clamping screw a to secure the tube firmly 1 2 TIP 3 4 5 6 2 Attaching the camer...

Page 43: ...er to fix the eyepieces 1 2 3 b a To attach the eyepiece micrometer loosen the clamping screw a of the right side eyepiece using a small flathead screwdriver and remove the eyepiece Hold the eyepiece frame loosen the reticle holder b and remove the reticle holder b Insert the eyepiece micrometer c in the removed reticle holder b with the display surface of the micrometer facing down Be careful not...

Page 44: ... of the slide glass with the high magnification objective 40X etc the objective may interfere with the specimen holder Only objectives of 24mm in diameter can be attached to all 5 holes of the revolving nosepiece For available objectives by observation method refer to the table Available observation methods by objective on page 35 Removing the objective If the objective is screwed in too firmly us...

Page 45: ...efore use When using the the specimen holder CX3 HLDT Insert the U shape groove of the specimen holder b in the specimen holder fixing knob a Picture B shown on the left While pushing the specimen holder toward the back arrow direction tighten the specimen holder fixing knob a 2 pcs You can also tighten the specimen holder fixing knob a by inserting the coin into the groove of the specimen holder ...

Page 46: ...C Electro Magnetic Compatibility performance of the product cannot be assured If no power cord is provided please select the proper power cord by referring to the section Proper selection of the power cord at the end of this instruction manual CAUTION The power cord and AC adapter cord are vulnerable when bent or twisted Never subject them to excessive force Be sure to set the main switch a to OFF...

Page 47: ...ching the allen wrench holder It is recommended to attach the provided allen wrench holder a to the back of the microscope frame The provided allen wrench b can be fixed to the provided allen wrench holder a TIP a b ...

Page 48: ...ly the power supply cord which is approved by one of the agencies mentioned in Table 1 please use replacements approved by any other equivalent and authorized agencies in your country Country Agency Certification mark Country Agency Certification mark Argentina IRAM Italy IMQ Australia SAA Japan JET JQA Austria ÖVE Netherlands KEMA Belgium CEBEC Norway NEMKO Canada CSA Spain AEE Denmark DEMKO Swed...

Page 49: ...ic Cables BASEC BASEC HAR 10 10 30 N V KEMA KEMA KEUR HAR 10 30 30 SEMKO AB Svenska Elektriska Materielkontrollanstalter SEMKO HAR 10 10 50 Österreichischer Verband für Elektrotechnik ÖVE ÖVE HAR 30 10 50 Danmarks Elektriske Materialkontroll DEMKO DEMKO HAR 30 10 30 National Standards Authority of Ireland NSAI NSAI HAR 30 30 50 Norges Elektriske Materiellkontroll NEMKO NEMKO HAR 10 10 70 Asociacio...

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