APPLIED OPERATIONS
/Image Acquisition
OPERATION INSTRUCTIONS
2 - 3 8
Page
2-2-2-2 XT Observation Mode
The description in this section will be focused on the image acquisition operations in the XT
observation mode that are not used in the XY observation modes (which are the operations
enclosed in
in the chart on the next page). For other operations, see section 2-2-1,
“Image Acquisition in XY Observation Mode”. The details of each operation will be described
in the subsequent sections.
Save image.
(Section 2-3-1 in [OPERATION])
Configure the microscope and scan unit.
(Sections 2-2-1-1 & 2-2-1-2 in [OPERATION])
Set the objective magnification.
(Section 2-2-1-4-1 in [OPERATION])
Set the dyeing method.
(Section 2-2-1-1 in [OPERATION])
Set the channel to be acquired.
(Section 2-2-1-4-3 in [OPERATION])
Set the highest scan speed.
(Section 2-2-1-4-4 in [OPERATION])
Perform repeated scanning.
(Section 2-2-1-4-6 in [OPERATION])
Set the XY observation mode.
(Section 2-2-1-4-5 in [OPERATION])
Set the observation range.
(Section 2-2-1-4-8 in [OPERATION])
Adjust the Z-position to observe the
desired cross-section.
(Section 2-2-1-4-7 in [OPERATION])
Set a lower scan speed.
(Section 2-2-1-4-10 in [OPERATION])
Stop repeated scanning.
(Section 2-2-1-4-11 in [OPERATION])
Set the observation mode.
(Section 2-2-2-2-1 in [OPERATION])
Acquire image.
(Section 2-2-2-2-4 in [OPERATION])
Set the observation line.
(Section 2-2-2-2-2 in [OPERATION])
Adjust the image brightness.
(Section 2-2-1-4-9 in [OPERATION])
Set the zoom ratio to 1X.
(Section 2-2-1-4-2 in [OPERATION])
If the image
does not
become
clean
If the image
becomes
clean
Re-adjust the image brightness.
(Section 2-2-1-4-9 in [OPERATION])
Set the number of scans.
(Section 2-2-2-2-3 in [OPERATION])
