APPLIED OPERATIONS
/Image Acquisition
OPERATION INSTRUCTIONS
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2-2-2-6 XYZT Observation Mode
The description in this section will be focused on the image acquisition operations in the
XYZT observation mode that are not used in the XY observation modes (which are the
operations enclosed in
in the chart on the next page). For other operations, see
section 2-2-1, “Image Acquisition in XY Observation Mode”. The details of each operation
will be described in the subsequent sections.
Save image.
(Section 2-3-1 in [OPERATION])
Configure the microscope and scan unit.
(Sections 2-2-1-1 & 2-2-1-2 in [OPERATION])
Set the zoom ratio to 1X.
(Section 2-2-1-4-2 in [OPERATION])
Set the objective magnification.
(Section 2-2-1-4-1 in [OPERATION])
Set the dyeing method.
(Section 2-2-1-1 in [OPERATION])
Set the channel to be acquired.
(Section 2-2-1-4-3 in [OPERATION])
Set the highest scan speed.
(Section 2-2-1-4-4 in [OPERATION])
Perform repeated scanning.
(Section 2-2-1-4-6 in [OPERATION])
Set the XY observation mode.
(Section 2-2-1-4-5 in [OPERATION])
Set the observation range.
(Section 2-2-1-4-8 in [OPERATION])
Set a lower scan speed.
(Section 2-2-1-4-10 in [OPERATION])
Re-adjust the image brightness.
(Section 2-2-1-4-9 in [OPERATION])
Stop repeated scanning.
(Section 2-2-1-4-11 in [OPERATION])
Set the observation mode.
(Section 2-2-2-6-3 in [OPERATION])
Acquire image.
(Section 2-2-2-6-6 in [OPERATION])
Set the numbers of Z-direction steps
and acquired image slices.
(Section 2-2-2-6-2 in [OPERATION])
Adjust the Z-position to observe the
desired cross-section.
(Section 2-2-1-4-7 in [OPERATION])
Adjust the image brightness.
(Section 2-2-1-4-9 in [OPERATION])
Set the interval time.
(Section 2-2-2-6-4 in [OPERATION])
Set the number of scans.
(Section 2-2-2-6-5 in [OPERATION])
If the image
does not
become
clean
If the image
becomes
clean
Set the range of the cross-section to
be observed (the Z-direction
scanning range).
(Section 2-2-2-6-1 in [OPERATION])
