APPLIED OPERATIONS
/Image Acquisition
OPERATION INSTRUCTIONS
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Page
2-2-3-3 Transmitted Image
Images obtained by transmitted light observation can also be acquired or observed
simultaneously with images obtained by fluorescence observation.
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When observing fluorescence images simultaneously, set the dyeing method.
1. From the page tabs on the bottom right of the [Acquire] panel, select the [Dyes]
sub-panel.
Fig. 2-22 [Dyes] Sub-panel
2. Select the specimen dyeing method by dragging desired dye names in the
[Available Dyes] list box in the [Selected Dyes] group box to the field immediately
above the list box.
3. Click the <Apply> button to apply the selected dyeing method to the [Ch] group
box on the upper part of the [Acquire] panel.
When the dyeing method is selected from the [Available Dyes] list box and the
<Apply> button is clicked, a channel for acquiring fluorescence is set
automatically according to the changed filter. And the dyeing method is shown
in the [Ch] group box.
[Available Dyes] list box
Lists the available dyes. Select the
desired items from this list and drag
them to the field above it to select the
dyeing method.
<Clear> button
Clear the set dyeing method.
<Prev.> button
Sets the dyeing method which was
set last time by clicking the <Apply>
button.
<Apply> button
Applies the dyeing method
dragged in the [Selected Dyes]
group box to the [Ch] group in
the [Acquire] panel.
Place the pointer on the icon
displayed in the [Selected Dyes],
and the dyeing method is shown in
the pop-up display.
TIP
[Assign dyes manually]
check box
Checking this enables the
manual setting. Dragging
the dyeing method in the
list directly to the [Ch]
group box assigns the
dye to the desired
channel.
