APPLIED OPERATIONS
/Image Acquisition
OPERATION INSTRUCTIONS
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2-2-12-2 Example of FRAP experiment
The example of procedure of FRAP experiment using AOTF is described in this section.
In order to perform photobleaching to a specimen, the value of laser intensity is raised to
100% to acquire an image since strong laser intensity is temporarily required. When 100% or
more of laser intensity value is required, zoom magnification is gathered to acquire an image.
(It is because the square power of zoom magnification per time can be secured.) Moreover,
using the objective of larger NA concentrates the laser irradiation spot in Z direction, and
stronger laser can be irradiated.
Therefore, a preliminary experiment is required to set up the laser intensity, the objective,
the zoom ration of the REX mask file according to a specimen.
1 Preliminary Experiment
1.
Set the laser intensity, the objective, and the zoom ration in the [Acquire] panel so that
they are suitable for a specimen.
2 Acquiring Image in XY mode
1.
Confirm that [XY-Norm] is displayed in the [Mode] group box in the [Acquire] panel.
2.
Select the [XY] option button in the [Acquire] panel.
