APPLIED OPERATIONS
/ Protocol processor
OPERATION INSTRUCTIONS
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2-4-8 Example of assembling a protocol
This section describes an example of assembling a protocol.
2-4-8-1 Example of Setting Procedure of FRAP Observation
An example of editing a protocol is described below assuming the FRAP observation.
Set Zoom
ratio to 2
An image
acquisition
Wait for
5 sec.
Image acquisition:
3 frames
(Photo bleaching)
Protocol
Track 1
Track 2
Track 3
Set Zoom
ratio to 10
Set Zoom
ratio to 2
Image
acquisition
Image
saving
Repeat 30 times
Image
acquisition
Wait for
5 sec.
Initial image acquisition: Track 1 sets the zoom ratio to 2 x, and acquires an image.
Photobleaching: Track 2 sets the zoom ratio to 10 x, and acquires image 3 times to make
photobleach on the specimen.
Recovery measurement: Track 3 sets the zoom ratio to 2 x, and acquire image 3 times with
5 seconds interval to observe the recovery. Then, stores the image.
Execute a XY repeated scanning and adjust the set values for image
acquisition before executing the protocol.
TIP
