Setting the Confocal Aperture
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3 Setting the Confocal Aperture
The confocal aperture position is determined by the combination of the excitation dichroic mirror
(hereinafter “excitation DM”) and spectral beam splitter (hereinafter “spectral BS”). If the confocal aperture
position has not be factory set for the combination of excitation DM and spectral BS that you selected, it
may become impossible to acquire correct images.
This chapter deals with how to adjust the confocal aperture position.
Follow the following.
For the procedure of each operation, refer to the section or manual indicated inside parentheses ( ).
Turn power on and start the FLUOVIEW software.
(Sections 1-2-1, “Turning Power ON” and 1-2-2, “Starting the Software” in Volume [OPERATION] of
FV1000 User’s Manual)
Perform settings in the [C.A. Adjustment] dialog box.
(Section 3-3)
While adjusting the focus on the mirror specimen,
adjust the confocal aperture position.
Set the combination of the excitation DM and spectral BS.
([Optical System Configuration] window)
Select the channel subjected to confocal aperture setting.
