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OPERATION INSTRUCTIONS
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One Point!
The formula of resolution of Z is as follows;
FWHM : Resolution of Z
L : The length of a diagonal line of Square Pinhole
NA = nSIN
θ
:NA of Objective lens
MG : The objective Magnification
N : The refractive index of a medium
Air : 1.0
Water : 1.3
Oil : 1.5
X = L/(1.22*3.8*
λ
*MG/NA)
The case where the variable x is as follows; 0 <= x < 2.0
FWHM = (2
λ
*ZPSF)/(4n(1-COS
θ
))
ZPSF = -5.5158*10
-2
x
5
+ 2.0877*10
-1
x
4
- 2.1172*10
-1
x
3
+ 2.0864*10
-1
x
2
- 2.2669*10
-2
x +
6.9038*10
-1
The case where the variable x is as follows; 2.0 <= x
FWHM = (2
λ
*ZPSF)/(4n(1-COS
θ
))
ZPSF = 0.6164x+0.1283
