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Olympus FV1200 User guide 

June 23, 2015 

3.

 

Enter the number of acquisition frame in 

Num

 box. 

4.

 

Click 

 button to start the timelapse. 

5.

 

Click 

SeriesDone

 

button to finish the sequence and save the image. 

 
 
 
 
I. Saving and Exporting Images

1.

 

Click on the image window to be saved.  
 

2.

 

Click on 

icon or select 

File/Save or Save as 

from 

menu.  

 

3.

 

A

 Save as

 window will appear. In case the image should 

be saved in the Image folder of a Log-in user, click on 

 

button and it will direct to your image folder.  

Select 

Olympus Image Binary Format *.oib

file type, 

type file name, and click on 

Save 

button. (Log-in user 

and its associate folder will be created as you become 
self-user).

 

 
4.

 

To set up your image folder where you can jump directly by 
clicking 

My Image Folder

 button, select 

Tools/Option

 

menu.  

Click 

General

tab and specify the path of your image folder 

with 

Browse..

button.  [

Upon creating a user account, each 

user will have its own folder in 

D

 drive (Do not create any 

folder in 

C

 drive.).  

Click OK button.  

 

5.

 

Oib file type contains all the metadata including all the 
acquisiotn parameters and it can be opened in FV10-ASW 
program. The light version of this program is available for 
installing in user’s own computer.   Otherwise, the image 
file can be exported as other file format so that it can be opened and processed in other imaging software.  

 

To export images: 

1.

 

Select 

File/Export

 or 

File/Export Multi-Tiff

 menu. Click 

My 

Image Folder 

button if the current folder is not yours.  

 

2.

 

With 

Export Multi-Tiff

 

option, it saves the image as a 

single tiff file that can contain multiple frames, like z- or 
timelapse- series.  This file type can be opened in Image J 
software.  

Summary of Contents for FV1200 MPE

Page 1: ...supply by pushing the ON OFF button and releasing it The light should lights up in Blue 3 Turn on microscope controller and XY stage controller 4 Turn on the touch panel controller and touch Start Ope...

Page 2: ...power supplies a Multi Ar laser Switch to ON and turn the Key to ON b MCPSU 405 440 473 559 635nm Switch ON c LD559 laser Switch on and WAIT UNTIL THE TEMPERATURE LIGHT STOPS BLINKING and THEN turn th...

Page 3: ...to 1x With zoomed view you can select the scanning area by moving the blue lined box around Laser power control Checking the box will activate the laser The power can be adjusted by pointing the mouse...

Page 4: ...e sample at 2us pixel because of its fast display refreshing rate this will be good for focusing and searching for ROIs without bleaching your sample Focus x4 will be faster but the display quality ma...

Page 5: ...jective completely away from the sample and press again to bring the objective to the original position Fluorescent image observation 1 Select an objective lens using the touch panel controller TPC 2...

Page 6: ...u selected If the Selected dyes upon opening the program are not ones that you want to use click the All Clear button first before selecting your dyes 2 Choose Image size default 512x512 from Size pan...

Page 7: ...ckground in blue e Click to stop the fast scanning and click to scan at the imaging speed and to check the image quality Adjust more if needed 7 Repeat the above procedure for 515 nm EYFP and for 635...

Page 8: ...one channel first and then scans the next channel If there is no bleed through between the emissions of some fluorophores you are using you can group them together to scan them simultaneously as a gro...

Page 9: ...f the objective you are using so that there is no gap between the optical sections upon projection into 3D The step size can be fixed by checking the box 5 Click Stop button Adjust the Scan Speed if n...

Page 10: ...icking My Image Folder button select Tools Option menu Click General tab and specify the path of your image folder with Browse button Upon creating a user account each user will have its own folder in...

Page 11: ...pe for export ROI Overlay section allows saving the file with ROI information Output Format RGB Color the red green and blue channel will be saved in color as individual tif files in a folder RGB Colo...

Page 12: ...hour or so leave the microscope system ON If not shut down the system as described below Shutting Off the system 1 Shut down the computer 2 Turn the mercury lamp off by pushing ON OFF button for two s...

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